Influence of cross-linkers on ezrin-bound minimal actin cortices

Schön, Markus; Mey, Ingo; Steinem, Claudia

doi:10.1016/j.pbiomolbio.2018.07.016

Cited by 8 publications

(2 citation statements)

References 62 publications

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“…Dynamic actin–membrane linkages have to date been reconstituted only on SLBs. Using ezrin recruited to the bilayer via PIP2 lipids, a dynamic actin network was created that could be remodeled by passive filament cross-linkers . Bead tracking microrheology showed that ezrin serves as a dynamic cross-linker for the membrane-attached actin layer, with the network stiffness being controlled by the pinning point density .…”

Section: Involving the Membranementioning

confidence: 99%

“…Using ezrin recruited to the bilayer via PIP2 lipids, a dynamic actin network was created that could be remodeled by passive filament cross-linkers. 172 Bead tracking microrheology showed that ezrin serves as a dynamic cross-linker for the membrane-attached actin layer, with the network stiffness being controlled by the pinning point density. 173 Ezrin-anchored actin filaments could diffuse over the membrane, but longer filaments were immobilized, being pinned by a larger number of actin–membrane links.…”

Section: Involving the Membranementioning

confidence: 99%

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Actomyosin-Driven Division of a Synthetic Cell

et al. 2022

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One of the major challenges of bottom-up synthetic biology is rebuilding a minimal cell division machinery. From a reconstitution perspective, the animal cell division apparatus is mechanically the simplest and therefore attractive to rebuild. An actin-based ring produces contractile force to constrict the membrane. By contrast, microbes and plant cells have a cell wall, so division requires concerted membrane constriction and cell wall synthesis. Furthermore, reconstitution of the actin division machinery helps in understanding the physical and molecular mechanisms of cytokinesis in animal cells and thus our own cells. In this review, we describe the state-of-the-art research on reconstitution of minimal actin-mediated cytokinetic machineries. Based on the conceptual requirements that we obtained from the physics of the shape changes involved in cell division, we propose two major routes for building a minimal actin apparatus capable of division. Importantly, we acknowledge both the passive and active roles that the confining lipid membrane can play in synthetic cytokinesis. We conclude this review by identifying the most pressing challenges for future reconstitution work, thereby laying out a roadmap for building a synthetic cell equipped with a minimal actin division machinery.

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Section: Involving the Membranementioning

confidence: 99%

Section: Involving the Membranementioning

confidence: 99%

Actomyosin-Driven Division of a Synthetic Cell

et al. 2022

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Benefits and challenges of reconstituting the actin cortex

Waechtler,

Jayasankar,

Morin

et al. 2024

Cytoskeleton

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The cell's ability to change shape is a central feature in many cellular processes, including cytokinesis, motility, migration, and tissue formation. The cell constructs a network of contractile proteins underneath the cell membrane to form the cortex, and the reorganization of these components directly contributes to cellular shape changes. The desire to mimic these cell shape changes to aid in the creation of a synthetic cell has been increasing. Therefore, membrane‐based reconstitution experiments have flourished, furthering our understanding of the minimal components the cell uses throughout these processes. Although biochemical approaches increased our understanding of actin, myosin II, and actin‐associated proteins, using membrane‐based reconstituted systems has further expanded our understanding of actin structures and functions because membrane–cortex interactions can be analyzed. In this review, we highlight the recent developments in membrane‐based reconstitution techniques. We examine the current findings on the minimal components needed to recapitulate distinct actin structures and functions and how they relate to the cortex's impact on cellular mechanical properties. We also explore how co‐processing of computational models with wet‐lab experiments enhances our understanding of these properties. Finally, we emphasize the benefits and challenges inherent to membrane‐based, reconstitution assays, ranging from the advantage of precise control over the system to the difficulty of integrating these findings into the complex cellular environment.

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A biophysical perspective of the regulatory mechanisms of ezrin/radixin/moesin proteins

Senju

Tsai

2022

Biophys Rev

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Influence of cross-linkers on ezrin-bound minimal actin cortices

Cited by 8 publications

References 62 publications

Actomyosin-Driven Division of a Synthetic Cell

Actomyosin-Driven Division of a Synthetic Cell

Benefits and challenges of reconstituting the actin cortex

A biophysical perspective of the regulatory mechanisms of ezrin/radixin/moesin proteins

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