Influence of cryopreservation methods of ex vivo rat and pig skin on the results of in vitro permeation test

Ling, Jiawei; Du, Ying; Sheng, Yuze; Wang, Weiqin; Wu, Hangyi; Chen, Guorong; Lv, Huixia

doi:10.1016/j.ejpb.2023.06.004

Cited by 5 publications

(1 citation statement)

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“…Cell viability was increased, and structural deformation was reduced when compared to SCS. However, Ling et al highlighted the importance of the storage media by comparing skin viability following cryopreservation in DMSO and glycerin, and without CPA, for 7 days at −20°C ( 60 ). If their study did not focus on preventing ice nucleation in supercooling temperatures, it highlights again the critical role of the CPA in ensuring cell homeostasis during subzero storage.…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Ex vivo preservation of transplanted organs is undergoing spectacular advances. Machine perfusion is now used in common practice for abdominal and thoracic organ transportation and preservation, and early results are in favor of substantially improved outcomes. It is based on decreasing ischemia-reperfusion phenomena by providing physiological or sub-physiological conditions until transplantation. Alternatively, supercooling techniques involving static preservation at negative temperatures while avoiding ice formation have shown encouraging results in solid organs. Here, the rationale is to decrease the organ's metabolism and need for oxygen and nutrients, allowing for extended preservation durations. The aim of this work is to review all advances of supercooling in transplantation, browsing the literature for each organ. A specific objective was also to study the initial evidence, the prospects, and potential applications of supercooling preservation in Vascularized Composite Allotransplantation (VCA). This complex entity needs a substantial effort to improve long-term outcomes, marked by chronic rejection. Improving preservation techniques is critical to ensure the favorable evolution of VCAs, and supercooling techniques could greatly participate in these advances.

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Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

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Physiologically‐based pharmacokinetic model of in vitro porcine ear skin permeation for drug delivery research

Krumpholz,

Polak,

Wiśniowska

2024

J of Applied Toxicology

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In silico techniques, such as physiologically based pharmacokinetic modeling (PBKP), are recently gaining importance. Computational methods in drug discovery and development and the generic drugs industry enhance research effectiveness by saving time and money and avoiding ethical issues. One key advantage is the ability to conduct toxicology studies without risking harm to living beings. This study aimed to repurpose the multi‐phase multi‐layer mechanistic dermal absorption (MPML MechDermA) PBPK model for simulation permeation through porcine ear skin under in vitro conditions. The work was divided into four steps: (1) the development of a pig ear skin model based on a previously collected dataset; (2) testing the model's ability to discriminate permeation between pig ear, human abdomen, and human back skin; (3) development of a caffeine permeation model; and (4) testing the caffeine model's performance against in vitro generated data sourced from the scientific literature. Data from 31 manuscripts were used for the development of the pig skin model. Based on these data, values specific to pig skin were found for 22 parameters of the MPML MechDermA model. The model was able to discriminate permeation between pig and human skin. A caffeine model was developed and used to simulate seven experiments identified in the literature. The model's performance was assessed by comparing simulated to observed results. Based on a visual check, all simulations were considered acceptable, whereas three out of seven experiments met the twofold difference criterion. The variability of the experimental data was considered the biggest challenge for reliable model assessment.

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Differences in the permeation of Licoricchalcone A-polysaccharide self-assembled nanoparticles on healthy and DNCB-induced atopic dermatitis in Balb/c mice

Chen,

Xue,

Wang

et al. 2024

International Journal of Biological Macromolecules

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Influence of cryopreservation methods of ex vivo rat and pig skin on the results of in vitro permeation test

Cited by 5 publications

References 50 publications

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Physiologically‐based pharmacokinetic model of in vitro porcine ear skin permeation for drug delivery research

Differences in the permeation of Licoricchalcone A-polysaccharide self-assembled nanoparticles on healthy and DNCB-induced atopic dermatitis in Balb/c mice

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