Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells

Aktanova, Alina; Boeva, O. S.; Barkovskaya, M. Sh.; Коваленко, Е. А.; Pashkina, Ekaterina

doi:10.3390/ijms24021441

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…In previous studies, we evaluated the possible immunomodulatory effect of cucurbiturils [ 30 , 31 , 32 , 33 ], and it was shown that during cultivation in the presence of CB[n], the proliferative activity of cells increased and the expression of HLA-DR on lymphocytes increased, which indicates an immunostimulatory effect. It was shown that cucurbiturils had practically no immunosuppressive effect under in vitro conditions, except for a slight decrease in HLA-DR expression and the production of reactive oxygen species by T-helpers in stimulated cultures [ 30 , 33 ]. However, for macrocyclic cavitands and for cucurbiturils it has been demonstrated that some properties only manifest themselves under in vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Immunosafety of Cucurbit[n]uril In Vivo

Pashkina,

Aktanova,

Boeva

et al. 2024

Pharmaceutics

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Cucurbiturils are a family of macrocyclic oligomers capable of forming host–guest complexes with various molecules. Due to noncovalent binding to drug molecules and low toxicity, cucurbiturils has been extensively investigated as potential carriers for drug delivery. However, the immune system’s interactions with different drug carriers, including cucurbiturils, are still under investigation. In this study, we focused on cucurbiturils’ immunosafety and immunomodulation properties in vivo. We measured blood counts and lymphocyte subpopulations in blood, spleen, and bone marrow, and assessed the in vivo toxicity to spleen and bone marrow cells after intraperitoneal administration to BALB/c mice. When assessing the effect of cucurbit[6]uril on blood parameters after three intraperitoneal injections within a week in laboratory animals, a decrease in white blood cells was found in mice after injections of cucurbit[6]util, but the observed decrease in the number of white blood cells was within the normal range. At the same time, cucurbit[7]uril and cucurbit[8]uril did not affect the leukocyte counts of mice after three injections. Changes in the number of platelets, erythrocytes, and monocytes, as well as in several other indicators, such as hematocrit or erythrocyte volumetric dispersion, were not detected. We show that cucurbiturils do not have immunotoxicity in vivo, with the exception of a cytotoxic effect on spleen cells after сucurbit[7]uril administration at a high dosage. We also evaluated the effect of cucurbiturils on cellular and humoral immune responses. We founded that cucurbiturils in high concentrations affect the immune system in vivo, and the action of various cucurbiturils differs in different homologues, which is apparently associated with different interactions in the internal environment of the body.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Immunosafety of Cucurbit[n]uril In Vivo

Pashkina,

Aktanova,

Boeva

et al. 2024

Pharmaceutics

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Nitrogen-doped carbon dots as a highly efficient photosensitizer for photodynamic therapy to promote apoptosis in oral squamous cell carcinoma

Sun,

Zhao,

Yan

et al. 2024

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Effect of cucurbiturils on the numbers and differential counts of peripheral blood leukocytes in laboratory animals after <i>in vivo</i> parenteral administration

Pashkina,

Aktanova,

Kovalenko

et al. 2023

Russian Journal of Immunology

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Immunological safety of nanoparticles is an urgent problem for development of drug delivery systems used as the basis for creating these systems. Cucurbit[n]urils (CB[n]) are molecular nanocontainers that can encapsulate various drugs and serve as the basis for delivery systems. Cucurbiturils are low-toxic compounds: under in vitro conditions, they exhibit weak immunomodulatory properties, without sufficient immunotoxicity. The aim of the present study was to evaluate the in vivo effects of cucurbiturils on the number and differential leukocyte counts in peripheral blood. BALB/c mice aged 2-4 months were used in the work. For this study, cucurbiturils were diluted in phosphate-buffered saline and administered to laboratory animals intraperitoneally (three times a week). When evaluating the effect of cucurbit[7]uril on blood parameters of the animals after three intraperitoneal injections weekly, no statistically significant changes were registered. However, at first administration, the animals showed a slight increase in relative number of lymphocytes after the first injection of CB[6], and an increased proportion of neutrophils after the first administration of CB[8]. Meanwhile, the proportions of lymphocytes and neutrophils were within normal ranges after the 2nd and 3rd injections of cucurbiturils, and did not show any significant differences against the controls. Moreover, the impact upon the subpopulation composition of peripheral blood lymphocytes in laboratory animals was assessed. After injection of CB[6], there was a decrease in T lymphocytes in the peripheral blood, along with increased proportion of CD19+B lymphocytes compared with the controls. CB[7] and CB[8] injections did not affect the subpopulation profile of peripheral blood lymphocytes. We have found that intraperitoneal administration of CB[n] did not affect the blood parameters of laboratory animals, thus, probably suggesting the in vivo safety of these compounds. At the same time, CB[6] is able to exert a stimulating effect on humoral immunity by increasing relative contents of B lymphocytes.

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Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells

Cited by 3 publications

References 57 publications

Evaluation of the Immunosafety of Cucurbit[n]uril In Vivo

Evaluation of the Immunosafety of Cucurbit[n]uril In Vivo

Nitrogen-doped carbon dots as a highly efficient photosensitizer for photodynamic therapy to promote apoptosis in oral squamous cell carcinoma

Effect of cucurbiturils on the numbers and differential counts of peripheral blood leukocytes in laboratory animals after <i>in vivo</i> parenteral administration

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