Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV

Abstract

Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Levenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on Levenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained from Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.

System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster

“…To infer position of RUS_B0 strain on the phylogenetic tree and its relationship with other members of the Beijing B0/W148 cluster, we performed a phylogenetic analysis. For this, we randomly selected 10 samples for each described sublineage of lineage 2 21 , as well as the maximum number of Beijing B0/W148 cluster strains from NCBI. Overall, 496 strains were used to construct a SNPs-based phylogenetic tree (Fig.…”