2009
DOI: 10.1007/s10295-009-0643-7
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Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304

Abstract: 23Among several lipase producing actinomycete strains screened, Amycolatopsis 24 mediterranei DSM 43304 was found to produce a thermostable, extracellular lipase. 25Culture condition and nutrient source modification studies involving carbon sources, 26 nitrogen sources, incubation temperature and medium pH were carried out. Lipase activity 27 of 1.37 ± 0.103 IU/ml of culture medium was obtained in 96 h at 28 °C and pH 7.5 using 28 linseed oil and fructose as carbon sources and a combination of phytone peptone … Show more

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Cited by 39 publications
(23 citation statements)
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“…Reports have shown that yeast extract can be dependable for large scale production of lipase enzyme (Dheeman et al, 2010). Yeast extract stimulated lipase production in the present study but was not generally found to be the best source This is in contrast with the observation of Dheeman et al, (2010); who reported the ability of yeast to stimulate maximum activity.…”
Section: Resultscontrasting
confidence: 99%
See 3 more Smart Citations
“…Reports have shown that yeast extract can be dependable for large scale production of lipase enzyme (Dheeman et al, 2010). Yeast extract stimulated lipase production in the present study but was not generally found to be the best source This is in contrast with the observation of Dheeman et al, (2010); who reported the ability of yeast to stimulate maximum activity.…”
Section: Resultscontrasting
confidence: 99%
“…Reports have shown that yeast extract can be dependable for large scale production of lipase enzyme (Dheeman et al, 2010). Yeast extract stimulated lipase production in the present study but was not generally found to be the best source This is in contrast with the observation of Dheeman et al, (2010); who reported the ability of yeast to stimulate maximum activity. Considering the effects of the various treatments involving nitrogen sources, the results showed that organic sources of nitrogen is a very important component for the growth of organism as appreciable growth values were observed with organic sources but not with inorganic sources.…”
Section: Resultscontrasting
confidence: 99%
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“…In all culture media, also, there were variations in activity during the cultivations, with a progressive reduction in enzyme activity, which may be due to the secretion of proteases at the end of the logarithmic phase (Dheeman et al, 2010).…”
Section: Identification Of Microorganism Strainsmentioning
confidence: 95%