Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media

Elmore, H. W.; Samples, B.; Sharma, Sanjeev; Harrison, M. D.

doi:10.1007/bf00114711

Cited by 13 publications

(5 citation statements)

References 19 publications

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“…Failure to prevent oxidation completely might be due to the decomposition of these acids in the media, as a result of their exposure to autoclaving, high light intensity and increasing pH. [13] Nonetheless, it should be noted that even highly oxidized explants and calli continued to produce healthy, non-oxidized callus for at least 15 subcultures. Photoperiod was not a key factor in callus induction, as explants developed this type of undifferentiated cell mass in both light conditions tested.…”

Section: Somatic Embryogenesis and Whole Plant Regeneration Of T Albamentioning

confidence: 83%

“…Oxidation of explants and callus was very common and increased with the number of subcultures, but could be controlled by supplementing the growth media with ascorbic and citric acid. Failure to prevent oxidation completely might be due to the decomposition of these acids in the media, as a result of their exposure to autoclaving, high light intensity and increasing pH . Nonetheless, it should be noted that even highly oxidized explants and calli continued to produce healthy, non‐oxidized callus for at least 15 subcultures.…”

Section: Resultsmentioning

confidence: 99%

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Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Krengel

Santoyo

Flores

et al. 2016

Chemistry & Biodiversity

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Tabernaemontana alba and Tabernaemontana arborea are Apocynaceae species used in Mexican traditional medicine for which little phytochemical information exists. In this study, preliminary gas chromatography/mass spectrometry analyses of different organs obtained from wild plants of both species identified a total of 10 monoterpenoid indole alkaloids (MIAs) and one simple indole alkaloid, nine of which were reported for the first time in these species. Furthermore, callus cultures were established from T. alba leaf explants and regeneration of whole plants was accomplished via somatic embryogenesis. The anti-addictive MIAs ibogaine and voacangine were then quantified by gas chromatography with flame ionization detection in wild plants of both species, as well as greenhouse-grown plants, in vitro-grown plantlets and embryogenic callus of T. alba. Ibogaine and voacangine were present in most samples taken from the whole plants of both species, with stem and root barks showing the highest concentrations. No alkaloids were detected in callus samples. It was concluded that T. alba and T. arborea are potentially viable sources of ibogaine and voacangine, and that these MIAs can be produced through somatic embryogenesis and whole plant regeneration of T. alba. Approaches to increase MIA yields in whole plants and to achieve alkaloid production directly in cell cultures are discussed.

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Section: Somatic Embryogenesis and Whole Plant Regeneration Of T Albamentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Krengel

Santoyo

Flores

et al. 2016

Chemistry & Biodiversity

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“…As most of the hardwood species produce phenolic compounds after wounding (George and Sherrington 1984), the same problem of leaching of phenols causing browning of explants has been reported in neem by Quraishi et al (2004) and also experienced in the present study. In order to check the phenolic exudation, explants right from the time of collection till inoculation were kept submerged either in water or antioxidant solution as it is well known that reduction in the direct contact of explants with air reduces the rate of oxidation of phenols at the wounded site (Elmore et al 1990). The explants were also given a pre-treatment with chilled antioxidant solution to further reduce the rate of oxidation of phenol.…”

Section: Resultsmentioning

confidence: 99%

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

et al. 2009

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An efficient in vitro process for rapid clonal propagation of a 40-year-old tree of Azadirachta indica employing nodal stem segments was developed. Season of collection and maturity of explants showed direct influence on bud-break. Nodal stem segments collected during the month of April gave best response. Maximum bud-break (78.6-81%) was obtained when middle order nodes (3rd or 4th node from apex) were taken. Amongst different cytokinins used, 6-benzylaminopurine (BAP) at the concentration of 1.11 lM was found most effective in inducing multiple shoots, whereas inorganic and organic constituents of the medium influenced growth and general condition of proliferating shoots. On an average 3.1 shoots per explant were regenerated in modified Murashige and Skoog medium supplemented with 1.11 lM BAP, 1.43 lM indole-3-acetic acid (IAA) and 81.43 lM adenine hemisulphate. Isolated shoots were rooted in presence of 2.46 lM indole-3-butryic acid (IBA). Root induction took place in 8-10 days with 100% rooting. The in vitro-raised plantlets were successfully transplanted in potted soil and finally grown under field conditions with 100% survival. The genetic fidelity of such in vitro-raised field-grown plants was ascertained by random amplified polymorphic DNA (RAPD) markers. Furthermore, the azadirachtin content of in vitro-cloned plants was found comparable to the mother tree proving their chemical stability also. The protocol developed holds good for in vitro cloning of mature elite neem trees.

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“…Ascorbic acid is known to decay rapidly in plant tissue culture media (Elmore et al 1990). When 0.05% ascorbic acid was added to MS medium before autoclaving, it was not effective in controlling the lethal browning (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid

Chen

et al. 2008

Plant Cell Tiss Organ Cult

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Cavendish banana cv. Formosana is a high yielding commercial cultivar resistant to race 4 of Fusarium oxysporum f. sp. cubense. Mass micropropagation of this cultivar has a serious problem of high mortality due to lethal browning of plantlets. The mineral contents in leaves and corms of diseased and healthy plantlets were similar. Amendment of culture medium with anion exchange resins, cation exchange resins, polyvinylpyrrolidone or activated charcoal did not reduce the disease incidence. However, addition of ascorbic acid to the surface of culture medium not only prevented the development of lethal browning but also greatly increased the number of plantlets produced. Even at 0.005% ascorbic acid was able to reduce the disease incidence by more than 60% and caused over 8-fold increase in number of plantlets produced. When cultures raised from 12 different Formosana corms were tested, ascorbic acid was able to reduce disease incidence by an average of 83%, and increase the number of plantlets in each test. When diseased plantlets were transferred to culture medium with ascorbic acid, all of them recovered, and resumed normal growth and multiplication, while all control plantlets on culture medium without ascorbic acid died after one month

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Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media

Cited by 13 publications

References 19 publications

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Quantification of Anti‐Addictive Alkaloids Ibogaine and Voacangine in In Vivo‐ and In Vitro‐Grown Plants of Two Mexican Tabernaemontana Species

Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments

Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid

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