Influence of culture conditions on growth and protective antigenicity of Clostridium chauvoei

Abstract

The effect of culture conditions on growth and immunogenicity of Clostridium chauvoei were examined. The pH control and partial feeding of the carbon source at high concentrations were beneficial for growth. The biomass yield was significatively improved, however the butanol concentration reached toxic levels hampering further growth. For each experimental condition the immunogenicity of cells was tested. No differences were found with cells obtained at different temperatures, but it decreased significatively with the partial supply of the carbon source and pH control.

Immunogenic protein variations ofClostridium chauvoeicellular antigens associated with the culture growth phase

“…The present study constitutes the first report that associates immunogenicity and protective capacity of C. chauvoei cellular antigens with the growth phases of batch cultures, being the immunogenic variations during growth more markedly in strains with lower protective capacity. We demonstrated the need to avoid the exposition of the cells to a lower final pH, final fermentation metabolites or glucose depletion that occurs at 24 h of incubation [10] corresponding to the late growth phase, because these culture conditions might negatively affect the protective capacity of the cells.…”

“…C. chauvoei strains were cultured in a clostridia medium previously described [10] under anaerobic conditions. The strains were grown in batch cultures in a 135‐ml fermenter with 100 ml of culture medium.…”

“…Moreover, the differential expression of genes in the stationary phase is well documented for prokaryotes [9]. Previous studies carried out in this laboratory have shown that the protective capacity of C. chauvoei cells diminishes when the cellular density is doubled by glucose partial feeding in batch cultures [10]. Nevertheless, no information is available so far as regards the variation of the protective antigens of C. chauvoei during growth.…”

Modulation of the immune response to an Aeromonas hydrophila aroA live vaccine in rainbow trout: effect of culture media on the humoral immune response and complement consumption

“…[8e15]. Modification of culture conditions can also increase the immunogenicity of killed [16,17] and live [18] vaccines.…”

Extracellular proteins of Clostridium chauvoei are protective in a mouse model

“…chauvoei strains were cultured in a Clostridium medium in batch system using the same conditions as previously described (Cortiñas et al, 1994). Culture growth was monitored by optical density determinations at 580 nm Acta Veterinaria Hungarica 55, 2007 (OD 580 ) with a Spectronic 20 spectrophotometer (Bausch & Lomb, New York, USA).…”