Influence of Culture Conditions on Lipopeptide Production by Bacillus subtilis

Akpa, Eric Essoh; Jacques, Philippe; Wathelet, Bernard; Paquot, Michel; Fuchs, Regine; Budzikiewicz, H.; Thonart, Philippe

doi:10.1007/978-1-4612-0217-2_47

Cited by 102 publications

(37 citation statements)

References 18 publications

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“…Since the fatty acid composition of the biosurfactant is controlled by the abundance of fatty acid precursors in the cell (1,5,6,11,16,17), we added exogenous branched-chain amino acids to the growth medium to determine the effect of changes in fatty acid composition on biosurfactant activity. The results of exogenous amino acid additions to the growth medium (Table 3) suggest that altering the ratio of even-numbered fatty acids has a more pronounced effect on specific activity than does altering the ratio of odd-numbered fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity

Youssef

Duncan

McInerney

2005

Appl Environ Microbiol

108

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Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r 2 ‫؍‬ 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r 2 ‫؍‬ 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.

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Section: Discussionmentioning

confidence: 99%

Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity

Youssef

Duncan

McInerney

2005

Appl Environ Microbiol

108

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“…[5,17 -19] To date, although with high difficulties, several studies have investigated the composition of bacterial lipopeptide extracts. Generally, Bacillus species co-produce various families of lipopeptides with different homologues and isoforms, as well summarised and demonstrated by Apka et al [20] Experiments were performed on the whole bacterial cell [10,21,22] or more often on the target molecules obtained by HPLC separation and then characterised by mass spectrometry. MALDI-TOF-MS analyses were reported by Williams et al [23] and Kim et al [24] to characterise fengycins obtained from Bacillus globigii and from Bacillusthuringiensis.…”

Section: Introductionmentioning

confidence: 99%

LC/ESI‐MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

et al. 2010

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Lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain showed an interesting anti-adhesion activity against biofilm formation of human pathogenic bacterial strains. The chemical characterisation of the crude extract of V9T14 strain was first developed through electrospray ionisation mass spectrometry (ESI-MS) and ESI-MS/MS direct infusions: two sets of molecular ion species belonging to the fengycin and surfactin families were revealed and their structures defined, interpreting their product ion spectra. The LC/ESI-MS analysis of the crude extract allowed to separate in different chromatogram ranges the homologues and the isoforms of the two lipopeptide families. The extract was then fractionated by silica gel chromatography in two main fractions, I and II. The purified biosurfactants were analysed through a new, rapid and suitable LC/ESI-MS/MS method, which allowed characterising the composition and the structures of the produced lipopeptides. LC/ESI-MS/MS analysis of fraction I showed the presence of C(13), C(14) and C(15) surfactin homologues, whose structures were confirmed by the product ion spectra of the sodiated molecules [M + Na](+) at m/z 1030, 1044 and 1058. LC/ESI-MS/MS analysis of fraction II confirmed the presence of two main fengycin isoforms, with the protonated molecules [M + H](+) at m/z 1478 and 1506 corresponding to C(17) fengycin A and C(17) fengycin B, respectively. Other homologues (C(14) to C(16)) were revealed and confirmed as belonging to fengycin A or B according to the retention times and the product ions generated, although with the same nominal mass. Finally, a relative percentage content of each homologue for both lipopeptides families in the whole extract was proposed.

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“…In addition, strain 629 increases magnesium content in the common bean shoots (Martins et al, 2015). The lipopeptides fengycin, surfactin and iturin are commonly produced by Bacillus species in common culture media (Akpa et al, 2001;Mukherjee and Das, 2005). In this study, it was found that B. amyloliquefasciens 629 produces the lipopeptides iturin, fengycin and surfactin in a temperature-and medium composition-dependent manner.…”

Section: Resultsmentioning

confidence: 71%

Effect of temperature, pH and substrate composition on production of lipopeptides by Bacillus amyloliquefaciens 629

Monteiro¹,

Medeiros²,

Ongena

et al. 2016

Afr. J. Microbiol. Res.

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The lipopeptides surfactin, fengycin and iturin produced by Bacillus species have diverse ecological roles, including antimicrobial activity, induced systemic resistance in plants against pathogens and plant colonization by the producing strain. The conditions that govern both lipopeptide production and plant colonization are not fully understood. The present study investigated the role of growth media, temperature and pH on the production of the lipopeptides surfactin, fengycin and iturin by Bacillus amyloliquefaciens 629 and its production in bean plants colonized epiphytically and endophytically by this isolate. Surfactin was produced at higher amounts when isolate 629 was grown at 15 than at 25 and 30ºC, whereas fengycin remained approximately constant across different temperatures. Iturin was detected on bean stem sap, root exudates and in potato dextrose broth, indicating that plant-derived nutrients play an important role in its production by isolate 629. B. amyloliquefaciens 629 colonized plants with more efficacy at 28 than at 20°C. None of the lipopeptides was detected in plants colonized by isolate 629, despite the number of attempts performed with ultra-performance liquid chromatography (UPLC) analysis.

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Influence of Culture Conditions on Lipopeptide Production by Bacillus subtilis

Cited by 102 publications

References 18 publications

Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity

Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity

LC/ESI‐MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain

Effect of temperature, pH and substrate composition on production of lipopeptides by Bacillus amyloliquefaciens 629

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