Influence of Culture Medium on Cementation of Coarse Grains Based on Microbially Induced Carbonate Precipitation

Gao, Hong; Shuhong, Dai

doi:10.3390/cryst12020188

Cited by 2 publications

(3 citation statements)

References 58 publications

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“…In this study, a robust prediction model was developed using RSM to enhance biofilm growth. However, according to a previous study 22 , the soil samples with different porosities need different biofilm growth rates; therefore, the RSM method should be correspondingly revised, which needs to be further studied. Moreover, several commonly used nutrients were considered in this RSM model, and various culture media were used in previous studies with different nutrients.…”

Section: Resultsmentioning

confidence: 99%

“…If the bonding points between coarse particles are effectively cemented, the sample would demonstrate significant solidification effects. To enhance the cementation effect at these bonding points, rapid formation and growth of biofilm is essential 21 , 22 . Precipitated CaCO 3 becomes attached to the biofilm, resulting in an increased amount of CaCO 3 precipitation available at the bonding points as the biofilm rapidly forms and grows, ultimately leading to improved cementation effects 23 – 26 .…”

Section: Introductionmentioning

confidence: 99%

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Optimization of culture medium to improve bio-cementation effect based on response surface method

Pan,

Cao

2024

Sci Rep

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The main challenge in the large-scale application of MICP lies in its low efficiency and promoting biofilm growth can effectively address this problem. In the present study, a prediction model was proposed using the response surface method. With the prediction model, optimum concentrations of nutrients in the medium can be obtained. Moreover, the optimized medium was compared with other media via bio-cementation tests. The results show that this prediction model was accurate and effective, and the predicted results were close to the measured results. By using the prediction model, the optimized culture media was determined (20.0 g/l yeast extract, 10.0 g/l polypeptone, 5.0 g/l ammonium sulfate, and 10.0 g/l NaCl). Furthermore, the optimized medium significantly promoted the growth of biofilm compared to other media. In the medium, the effect of polypeptone on biofilm growth was smaller than the effect of yeast extract and increasing the concentration of polypeptone was not beneficial in promoting biofilm growth. In addition, the sand column solidified with the optimized medium had the highest strength and the largest calcium carbonate contents. The prediction model represents a platform technology that leverages culture medium to impart novel sensing, adjustive, and responsive multifunctionality to structural materials in the civil engineering and material engineering fields.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of culture medium to improve bio-cementation effect based on response surface method

Pan,

Cao

2024

Sci Rep

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“…Post-MICP treatment, the aggregate properties were improved. Gao and Dai [9] suggested optimizing the medium to enhance the cementing effect. Optimizing the medium increased calcium carbonate precipitation at the contact point, enhanced the cementing effect, and increased strength by 1.2-4 times.…”

Section: Introductionmentioning

confidence: 99%

Using Electric Field to Improve the Effect of Microbial-Induced Carbonate Precipitation

Deng

Tian

et al. 2023

Sustainability

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The precipitation of calcium carbonate induced by Sporosarcina pasteurii (S. pasteurii) has garnered considerable attention as a novel rock and soil reinforcement technique. The content and structure of calcium carbonate produced through this reaction play a crucial role in determining the rocks’ and soil’s reinforcement effects in the later stages. Different potential gradients were introduced during the bacterial culture process to enhance the performance of the cementation and mineralization reactions of the bacterial solution to investigate the effects of electrification on the physical and chemical characteristics, such as the growth and reproduction of S. pasteurii. The results demonstrate that the concentration, activity, and number of viable bacteria of S. pasteurii were substantially enhanced under an electric field, particularly the weak electric field generated by 0.5 V/cm. The increased number of bacteria provides more nucleation sites for calcium carbonate deposition. Moreover, as the urease activity increased, the calcium carbonate content generated under an electric potential gradient of 0.5 V/cm surpassed that of other potential gradient groups. The growth rate increased by 9.78% compared to the calcium carbonate induced without electrification. Significantly, the suitable electric field enhances the crystal morphology of calcium carbonate and augments its quantity, thereby offering a novel approach for utilizing MICP in enhancing soil strength, controlling water pollution, and mitigating seepage. These findings elevate the applicability of microbial mineralization in engineering practices.

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Influence of Culture Medium on Cementation of Coarse Grains Based on Microbially Induced Carbonate Precipitation

Cited by 2 publications

References 58 publications

Optimization of culture medium to improve bio-cementation effect based on response surface method

Optimization of culture medium to improve bio-cementation effect based on response surface method

Using Electric Field to Improve the Effect of Microbial-Induced Carbonate Precipitation

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