Influence of cyclodextrins on the physical stability of DPPC-liposomes

X-ray grazing incidence diffraction and Langmuir monolayer studies of the interaction of β-cyclodextrin with model lipid membranes

“…Ohvo and Slotte [23] demonstrated that the desorption rates of various sterols from the surface film to b-CD solution depend on the relative polarity of sterols and increase considerably with the surface pressure rise. Moreover, sterol desorption from cholesterol/phospholipid mixed systems was found to be notably retarded, even though the removal of phospholipid molecules was not observed [23][24][25][26][27][28][29][30]. On the contrary, phospholipids' desorption from monolayers has recently been described by other authors [31].…”

“…Langmuir monolayers [21][22][23][24][25][26] and lipid vesicles [27][28][29]. The depletion of membrane constituents, namely cholesterol, phospholipids and even proteins from cells was described in several papers.…”

Fine-tuning of POPC liposomal leakage by the use of β-cyclodextrin and several hydrophobic guests

“…Apparently, β-CD acts as a destabilizing agent for POPC liposomes, probably by including POPC (Puskás and Csempesz, 2007;Irie et al, 1992) molecules into its cavity and, consequently, causing a reorganization of the molecular packing in the liposomal membrane. Clear evidence of the tendency of POPC molecules to form complexes with β-CD has been obtained from ESI-MS experiments (see Figure 4), in agreement with recent findings, through binding isotherms, on the interaction between various CDs and DPPC (1,2-dipalmitoylsn-glycero-3-phosphocholine) SUV liposomes (Puskás et al, 2006).…”

Liposome collapse resulting from an allosteric interaction between 2,6-dimethyl-β-cyclodextrins and lipids

“…It is noteworthy that Csempesz et al reported the same phenomenon for the mixing of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and a-CDx. 16 It can be difficult to analyse the nature of the interactions between DMPC and a-CDx because the resulting precipitate is insoluble in water. Furthermore, the aqueous solubility of b-CDx was too low to measure the intensity of its light scattering at 450 nm by UVvis absorption spectroscopy under the same conditions ([b-CDx]/[DLPC] ¼ 0-5 equiv.)…”