Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells

Siddiqui, Maqsood A.; Singh, Gaurav; Kashyap, Mahendra; Khanna, Vinay K.; Yadav, Sanjay; Chandra, Deepak; Pant, Aditya B.

doi:10.1016/j.tiv.2008.07.001

Cited by 129 publications

(83 citation statements)

References 37 publications

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“…Cells were grown in 5% CO 2 at 37 o C in high humid atmosphere. Before the experiments, viability of cells was assessed following the protocol of (Siddiqui et al, 2008). A-549 cells showing more than 95% cell viability and passage number between 6 and 8 were used in the present study.…”

Section: Cell Culturementioning

confidence: 99%

See 1 more Smart Citation

Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

Al-Sheddi¹,

Farshori²,

Al-Oqail³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Cell Culturementioning

confidence: 99%

“…MTT assay: percent cell viability was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay as described (Siddiqui et al, 2008). Briefly, A-549 cells (1510 4 ) were allowed to adhere for 24 h CO 2 incubator at 37°C in 96 well culture plates.…”

Section: Cytotoxicity Screeningmentioning

confidence: 99%

Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

Al-Sheddi¹,

Farshori²,

Al-Oqail³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

“…Cells were grown in 5% CO 2 at 37 0 C in high humid atmosphere. Before the experiments, viability of cells was assessed following the protocol of (Siddiqui et al, 2008). Cells showing more than 95% cell viability and passage number between 12 and 18 were used in the present study.…”

Section: Cell Lines and Cell Culturesmentioning

confidence: 99%

“…MTT assay: Percent cell viability was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay as described (Siddiqui et al, 2008). Briefly, cells (1x10 4 ) were allowed to adhere for 24 h CO 2 incubator at 37°C in 96 well culture plates.…”

Section: Cytotoxicity Screeningmentioning

confidence: 99%

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

Al-Oqail

Farshori²,

Al-Sheddi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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In the present study, investigations were carried out to screen the anticancer activities of fenugreek seed oil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity was assessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrast light microscopy. All cells were exposed toi 10-1000 μg/ml of fenugreek seed oil for 24 h. The results show that fenugreek seed oil significantly reduced the cell viability, and altered the cellular morphology in a dose dependent manner. Among the cell lines, HEp-2 cells showed the highest decrease in cell viability, followed by MCF-7, WISH, and Vero cells by MTT and NRU assays. Cell viability at 1000 μg/ml was recorded as 55% in HEp-2 cells, 67% in MCF-7 cells, 75% in WISH cells, and 86% in Vero cells. The present study provides preliminary screening data for fenugreek seed oil pointing to potent cytotoxicity against cancer cells.

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“…Cells were grown in 5% CO 2 at 37 o C in high humid atmosphere. Before the experiments, viability of cells was assessed following the protocol of (Siddiqui et al, 2008). A-549 cells showing more than 98% cell viability and passage number between 10 and 12 were used in this study.…”

Section: Cell Line and Cell Culturementioning

confidence: 99%

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

Al‐Taweel

Perveen²,

Fawzy³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to 10-1000 μg/ml concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at 1000 μg/ml each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.

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Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells

Cited by 129 publications

References 37 publications

Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells

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