Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: A comparative study

Condé-Green, Alexandra; Amorim, Natale F. Gontijo de; Pitanguy, Ivo

doi:10.1016/j.bjps.2009.07.018

Cited by 203 publications

(122 citation statements)

References 31 publications

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“…The SVF was isolated from human liposuction aspirates, and 0.5 ml adipose tissue was mixed with 1 × 10 6 SVF cells or culture medium then injected to nude mice subcutaneously. At 1,4,7,14,30, 60, and 90 days after transplantation, samples were harvested for 1) general observation and retention rate; 2) whole-mount stain; 3) H&E stain; 4) immunohistochemical staining for S100, CD68, and CD34; 5) ELISA for VEGF and bFGF; 6) peroxisome proliferator-activated receptor-g (PPARg) fluorescence in situ hybridization. The retention rate in the experiment group was markedly higher than that in the control group.…”

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confidence: 99%

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

et al. 2015

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Our objective was to explore the mechanism of cell-assisted adipose transplantation by using freshly isolated human stromal vascular fraction (SVF) cells and to observe the dynamic changes of the graft after transplantation. The SVF was isolated from human liposuction aspirates, and 0.5 ml adipose tissue was mixed with 1 × 10 6 SVF cells or culture medium then injected to nude mice subcutaneously. At 1,4,7,14,30, 60, and 90 days after transplantation, samples were harvested for 1) general observation and retention rate; 2) whole-mount stain; 3) H&E stain; 4) immunohistochemical staining for S100, CD68, and CD34; 5) ELISA for VEGF and bFGF; 6) peroxisome proliferator-activated receptor-g (PPARg) fluorescence in situ hybridization. The retention rate in the experiment group was markedly higher than that in the control group. Whole-mount stain shows most of the cells in the center of the graft could not survive the ischemia until day 14. Histology showed new vessels on the surface of the graft at 3 days. However, in the control group, fewer newly formed vessels were detected until day 7. In the late stage of transplantation, gradual fibrosis was found in the graft, and the tissue was divided into a grid-like structure. A large number of round neonatal adipocytes with big nuclei in the center were found surrounding the new vessels, which were S100 and CD34 positive and CD68 negative. In the late stage of transplantation, most of the neonatal adipocytes were human PPARg positive. Moreover, the SVF group showed a higher level of VEGF and bFGF. SVF assisting adipose transplantation could increase the retention rate of the graft through promoting adipose tissue regeneration via secretion of growth factors, promotion of angiogenesis, and increasing the density of mesenchymal stem cells.

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confidence: 99%

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

et al. 2015

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“…[6][7][8][9][10][11][12] There is no agreement as to the best way of processing the fat to ensure maximum take and viability of the graft. 7 We use the decantation technique that, compared to centrifugation, is arguably less selective for cell stems, but has the advantage of being more productive (0.7 l of fat tissue for 1 l of liposuction vs 0.5 l), and considerably quicker (30 min vs up to 2 h) following the use of closed systems that do not require fat manipulation other than a transfer to the injection syringes. 11,16 Using this technique, the whole procedure is performed in less than 90 min, minimizing the surgery-associated risks for the patients.…”

Section: Discussionmentioning

confidence: 99%

“…It is autologous, soft, frequently abundant and easily collected, but it is not inert: injection of fat in tissue may change the microenvironment by the addition of multipotent cells and stimulating factors. [6][7][8][9][10][11][12] Recently, fat grafting has been described for the treatment of early stage ischial or sacral pressure ulcers in patients with SCI and other chronic bedridden patients, with good results at 3 months follow-up. 13 We present the preliminary results of lipofilling as a secondary prevention of recurrence of ischial tuberosity and pelvic PrUs in patients with unsatisfactory outcome of previous surgical flap procedures.…”

Section: Introductionmentioning

confidence: 99%

Lipofilling (fat grafting) in the secondary prevention of ischial tuberosity and pelvic pressure ulcers

Prévinaire

Opsomer

et al. 2015

Spinal Cord

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Study Design: Retrospective study. Objectives: Lipofilling was proposed to adult chronic spinal cord injury patients with history of ischial tuberosity pressure ulcers surgery, at risk of recurrence of pressure ulcers due to unsatisfactory adipose tissue thickness. Setting: Fondation Hopale, Berck, France. Methods: The three staged (Coleman) procedure for fat grafting consisted of water-jet assisted liposuction (Harvest-Jet), decantation, and reinjection of the autologous fat in three-dimensional plan. Results: Ten consecutive patients (eight paraplegics and two tetraplegics) benefited from bilateral ischial lipofilling, with additional lipofilling of the sacrum (three patients) and the trochanters (two patients). All patients attended the seating clinic with pressure mapping before and after lipofilling for appropriate cushion prescription. Mean follow-up time was 16 months (4-24 months). Complications were limited to stage I (two patients) and stage II (one patient) pressure ulcers, due to negligence. Excellent results with minimal fat wasting (between 10% and 25%) underneath the ischial tuberosity were seen in eight patients, with significant improvement of adipose tissue thickness. Total fat waste was seen in two patients, one of whom following dramatic weight loss. Results were always good in the sacrum and trochanteric areas. Lipofilling allowed longer sitting times (three patients), improved skin quality (nine patients), improved quality of life (six patients), 'a better feeling of positioning in their wheelchair' (four patients), and decrease in pelvic pain (three patients). Discussion and Conclusion: Pelvic lipofilling is safe, cost-effective, and can now be considered in the primary prevention of pelvic pressure ulcers in patients with insufficient adipose tissue.

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“…Blood must be extracted because blood accelerates the degradation of the transplanted fat (37). Centrifugation based processing resulted in higher ADSC numbers but decreased cell viability counts than decantation (38). When centrifugation is used, several of the articles suggested that forces greater than 3000 rpm (1200 g) cause more cellular damage (7).…”

Section: Processingmentioning

confidence: 99%

Autologous fat transplantation for secondary breast reconstruction: our experience

Simonacci¹

2017

GHIR

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Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: A comparative study

Cited by 203 publications

References 31 publications

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

Lipofilling (fat grafting) in the secondary prevention of ischial tuberosity and pelvic pressure ulcers

Autologous fat transplantation for secondary breast reconstruction: our experience

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