Influence of decavanadate on rat synaptic plasma membrane ATPases activity

Krstić, Danijela; Čolović, Mirjana B.; Bošnjaković-Pavlović, Nada; A, Spasojević-De Bire; Vasić,

doi:10.4149/gpb_2009_03_302

Cited by 18 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Inhibitions of several adenosine triphosphatases, such as P-type ATPases have been shown [ 158 ]. The effect of V 10 on synaptic plasma membrane (SPM) and commercial porcine cerebral cortex Na, K-ATPase activity was investigated by in vitro exposure to the enzymes [ 159 ]. The results show that V 10 induce inhibition of enzymatic activity in a concentration-dependent manner in both cases ( Fig.…”

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 82%

“…V 10 induced signifi cant decrease of enzyme maximal velocity ( V max ), as well as decrease of its apparent affi nity for the substrate (ATP) (increased K m value) (Table 24.4 and Fig. 24.11 ) [ 159 ]. Table 24.4 The inhibition parameters of the SPM and commercial Na, K-ATPase inhibition by V 10 obtained by fi tting the experimental points using sigmoidal function and Hill analysis.…”

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 99%

“…The IC 50 values of the investigated compound for both samples ( a ) The concentrationdependent inhibition, and ( b ) Hill analysis of SPM Na, K-ATPase ( square ) and commercial porcine cerebral cortex Na, K-ATPase ( asterisk ) by V 10 [ 159 ] were determined by sigmoidal fi tting the experimental results (Fig. 24.10b ) as well as by Hill analysis and are summarized in Table 24.4 [ 159 ]. The nature of commercial porcine cerebral cortex Na, K-ATPase inhibition by V 10 was characterized as mixed type of inhibition.…”

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 99%

See 2 more Smart Citations

Metal Based Compounds, Modulators of Na, K-ATPase with Anticancer Activity

Momić

Čolović

Lazarević‐Pašti

et al. 2015

Regulation of Membrane Na+-K+ ATPase

View full text Add to dashboard Cite

Involvement of Na, K-ATPase in different biological processes and its overexpression in pathological states enables its use as a target in anticancer studies. For the past 10 years, a variety of metal-based complexes have been synthesized which offer good tolerance, potent action, selectivity, and less toxicity in cancer treatment. This chapter gives an overview of the interaction of platinum, gold, ruthenium, vanadium, and palladium complexes with Na, K-ATPase and their effect on the enzyme function and activity. The mechanism of Na, K-ATPase activity inhibition with metal based complexes is supported with extensive kinetic analysis. The inhibition can be achieved via the complexes interaction with -SH groups of the enzyme and cleavage of the disulfi de bridges, required for the enzyme functionality. Moreover, the inhibitory effect of selected compounds can be prevented and recovered by the addition of -SH donors, L -cysteine and glutathione, the biomolecules usually present in physiological liquids. The conclusion is made that gold, ruthenium, and palladium complexes are expected to overcome platinum complexes toxic side effects.

show abstract

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 82%

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 99%

Section: Inhibition Of Na K-atpase Activity With Vanadate Complexesmentioning

confidence: 99%

See 1 more Smart Citation

Metal Based Compounds, Modulators of Na, K-ATPase with Anticancer Activity

Momić

Čolović

Lazarević‐Pašti

et al. 2015

Regulation of Membrane Na+-K+ ATPase

View full text Add to dashboard Cite

show abstract

“…7 The energy that maintains the electrochemical gradient comes from the hydrolysis of ATP to ADP. It is the energy in this gradient that drives other membrane transport, cotransport, and exchange systems; allows the movement of cations, anions, amino acids, and glucose across the cell membrane; and maintains vital cellular functions including membrane electrical potential, cell volume, and intracellular Na , and other ion through cells membrane in relevant organizations, as can result in hyperkalemia, calcipenic, cellular swelling, and depolarization of membrane potential and ultimately aggravate dysfunction and morphological damage of cells.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5] Myeloperoxidase (MPO) levels are a reliable and quantitative marker for polymorphonuclear neutrophil (PMN) accumulation in tissues, 6 and membrane pump activity is an indicator for energy metabolism and ion transport of cells. 7 To test this hypothesis and to improve our understanding of the cellular mechanisms underlying the heart and pancreas injury caused by ARF and determined by the MPO and membrane pump activities in heart, pancreas, and kidney, we used our established model of tissue from rabbits subjected to ARF. In addition, this study found that the MPO activities were increased and the adenosine triphosphatase (ATPase) activities were decreased in heart and pancreas; as a result, the PMN sequestration and membrane pump suppression might be a major mechanism of myocardium and pancreas injury induced by ARF.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities

et al. 2010

View full text Add to dashboard Cite

LABORATORY STUDYThe mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activitiesThe mechanism of myocardium and pancreas injury in acute renal failure Zi-Gang Zhao, Chun-Yu Niu, Yu-Ping Zhang, Rui Han, Ya-Li Hou, Bao-liang Li, and Shu-ting Du Institute of Microcirculation, Hebei North University, Zhangjiakou 075000, Hebei, PR China AbstractThere is increasing evidence indicating that the distant organ injury is a major contributor of high mortality in patients subjected to acute renal failure (ARF). However, sources and mechanisms that ARF causes distant organ injury remain to be determined. The aim of this study is to explore the mechanism from polymorphonuclear neutrophil (PMN) sequestration and membrane pump suppression. To achieve this, we examined myeloperoxidase (MPO), a marker of PMN accumulation in tissues, and membrane pump activities of heart, pancreas, and kidney in two ARF rabbit models. Rabbits are randomly assigned to control, HgCl 2 -treated, and glycerin-treated groups. ARF animal models are established by hypodermic injection of 1% HgCl 2 with 1.3 mL/kg bodyweight (bw) in HgCl 2 -treated group or intramuscular injection of 50% glycerin with 10 mL/kg bw in glycerin-treated group, respectively, and all animals in each group are further divided into 12 h, 24 h, and 48 h subgroups with each consisting of six rabbits. Six healthy rabbits serve as control group. Results have shown that MPO activities of kidney, myocardium, and pancreas in two model groups were significantly increased than control group at diverse time points. Membrane pump activities of kidney in two model groups are significantly lower than the control group at multiple time points. Moreover, Na -ATPase activities of myocardium and pancreas in two model groups are gradually declined with the development of ARF. These findings suggest that PMN sequestration and membrane pump suppression plays an important role in the pathogenesis of ARF and also a major mechanism of myocardium and pancreas injury during the process of ARF.

show abstract

Therapeutic Potentials of Ecto‐Nucleoside Triphosphate Diphosphohydrolase, Ecto‐Nucleotide Pyrophosphatase/Phosphodiesterase, Ecto‐5′‐Nucleotidase, and Alkaline Phosphatase Inhibitors

al‐Rashida

Iqbal

2013

Medicinal Research Reviews

View full text Add to dashboard Cite

The modulatory role of extracellular nucleotides and adenosine in relevance to purinergic cell signaling mechanisms has long been known and is an object of much research worldwide. These extracellular nucleotides are released by a variety of cell types either innately or as a response to patho-physiological stress or injury. A variety of surface-located ecto-nucleotidases (of four major types; nucleoside triphosphate diphosphohydrolases or NTPDases, nucleotide pyrophosphatase/phosphodiesterases or NPPs, alkaline phosphatases APs or ALPs, and ecto-5'-nucleotidase or e5NT) are responsible for meticulously controlling the availability of these important signaling molecules (at their respective receptors) in extracellular environment and are therefore crucial for maintaining the integrity of normal cell functioning. Overexpression of many of these ubiquitous ecto-enzymes has been implicated in a variety of disorders including cell adhesion, activation, proliferation, apoptosis, and degenerative neurological and immunological responses. Selective inhibition of these ecto-enzymes is an area that is currently being explored with great interest and hopes remain high that development of selective ecto-nucleotidase inhibitors will prove to have many beneficial therapeutic implications. The aim of this review is to emphasize and focus on recent developments made in the field of inhibitors of ecto-nucleotidases and to highlight their structure activity relationships wherever possible. Most recent and significant advances in field of NTPDase, NPP, AP, and e5NT inhibitors is being discussed in detail in anticipation of providing prolific leads and relevant background for research groups interested in synthesis of selective ecto-nucleotidase inhibitors.

show abstract

Influence of decavanadate on rat synaptic plasma membrane ATPases activity

Cited by 18 publications

References 31 publications

Metal Based Compounds, Modulators of Na, K-ATPase with Anticancer Activity

Metal Based Compounds, Modulators of Na, K-ATPase with Anticancer Activity

The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities

Therapeutic Potentials of Ecto‐Nucleoside Triphosphate Diphosphohydrolase, Ecto‐Nucleotide Pyrophosphatase/Phosphodiesterase, Ecto‐5′‐Nucleotidase, and Alkaline Phosphatase Inhibitors

Contact Info

Product

Resources

About