Influence of Deep Hypothermia on the Tolerance of the Isolated Cardiomyocyte to Ischemia–Reperfusion

Characterization of antiproliferative and cytotoxic properties of the HSV‐1 immediate‐early ICP0 protein

“…Although it is, of course, impossible to investigate any antiproliferative action of ICP0 on nondividing cells, we have carefully studied whether ICP0 was cytotoxic for rat primary cardiomyocytes and rat primary nerve cells, two well-established models of non-cycling cells [23,24]. The crystal violet staining experiments indicated that most cardiomyocytes remained alive for more than a week after infection with the amplicon expressing ICP0.…”

“…HFFF-2 (human fetal foreskin fibroblasts, kindly obtained from Dr. R. Everett, Glasgow, Scotland) were propagated in DMEM supplemented with 5% FBS, 5% newborn bovine serum (Gibco), penicillin (100 U/mL) and streptomycin (100 µg/mL). Cultures of rat primary neonatal cardiomyocytes were seeded and maintained as already described [23], until infection. Primary cultures of rat neonatal brain cells were seeded on poly-L-lysinecoated coverslips and maintained as already described until infection [24,25].…”

Three‐Dimensional Geometry of Honeycomb Collagen Promotes Higher Beating Rate of Myocardial Cells in Culture

“…After anesthesia, their hearts were removed. The myocardial cells of the ventricular tissues were separated by enzymatic digestion and mechanical stimulus as previously described (8). The purified cells (6 × 10 5 /mL) were inoculated into 24‐well culture plates containing discs of HC (2–3 mm thick, 7 mm diameter).…”