Influence of Dehydrocholic and Cholic Acids on the Biliary Secretion of Anionic Polypeptide Fraction, the Major Apoprotein of the Biliary Lipoprotein Complex

Chanussot, F.; Domingo, N.; Tuchweber, Béatriz; Lafont, Huguette; Yousef, Ibrahim M.

doi:10.3109/00365529208999956

Cited by 15 publications

(3 citation statements)

References 26 publications

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“…The much more effective dose of 6% PC results in a significant increase in bile salts (such an increase could be associated with the ability to induce the micellar phase, as described elsewhere) (49) and a significant decrease in the CSI, suggesting a regulatory effect of the CSI in the effect of this level of PC supplementation. The decrease in APF level was shown previously in rats infused with cholic acid (50). Thus, we speculate that increasing the micellar phase may act by lowering the APF level and vesicular phase.…”

Section: Downloaded Frommentioning

confidence: 54%

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Kasbo

Tuchweber

Perwaiz

et al. 2003

Journal of Lipid Research

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Cholesterol gallstones affect approximately 10-15% of the adult population in North America. Phosphatidylcholine (PC) is considered to be the main cholesterol solubilizer in bile. This study examined the effect of a PCenriched diet on gallstone incidence in mice susceptible to cholelithiasis. The result obtained showed that the feeding of a lithogenic (LG) diet for 4 weeks or 8 weeks resulted in cholesterol gallstone incidences of 47% and 89%, respectively. These gallstone incidences were either reduced or prevented when the LG diet was enriched with 2% or 6% PC, respectively. The cholesterol saturation index (CSI) was reduced only in mice fed with LG ؉ 6% PC diet as compared with mice fed the LG diet alone. However, in all groups, the CSI was significantly higher than in mice fed Purina chow diet. The biliary anionic polypeptide fraction (APF) was significantly increased in mice fed the LG ؉ 2% PC diet and was reduced in those fed with LG ؉ 6% PC diet. In conclusion, prevention or delay of gallstone formation was not due to a consistent effect on biliary lipid composition, suggesting a direct effect of PC on cholesterol solubilization and/or the effect of an additional nonlipid biliary component such as APF.

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Section: Downloaded Frommentioning

confidence: 54%

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Kasbo

Tuchweber

Perwaiz

et al. 2003

Journal of Lipid Research

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“…However, in this study, the level of TUDCA was found to be elevated in NASH mice. Chanussot et al (1992) found that DHCA can inhibit the secretion of phospholipids and cholesterol to achieve the effect of protecting the liver. Similar results were found in the present study.…”

Section: Kaempferol Improves Nash By Regulating the Homeostasis Of Ba...mentioning

confidence: 99%

Kaempferol attenuates nonalcoholic steatohepatitis by regulating serum and liver bile acid metabolism

Lü

Shao²,

Zhang³

et al. 2022

Front. Pharmacol.

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Objective: Changes in bile acids (BAs) are increasingly recognized as potential targets for non-alcoholic steatohepatitis (NASH). Kaempferol has been proved to be anti-inflammatory and reduce the disorder of lipid metabolism. In order to analyze the BA profile in NASH mice and determine the predictive biomarkers of kaempferol treatment, serum-targeted metabolomics and liver tissue RNA sequencing (RNA-seq) were carried out.Design: Six normal control mice (NC group), eight HFD-fed mice (HFD group), and eight kaempferol-treated HFD-fed mice (HFD + KP group) were included in the present study. Ultra-performance liquid chromatography coupled to a tandem mass spectrometry system (UPLC-MS/MS) was used to quantify serum and liver BAs, and RNA-seq was used to quantify liver differentially expressed genes related to BA metabolism.Results: The serum levels of CA, βMCA, UDCA, and 12-DHCA, as well as ωMCA in both the serum and liver, were significantly decreased in the HFD group compared with those in the NC group, and kaempferol can increase the serum levels of βMCA, UDCA, and ωMCA and the liver level of 12-DHCA. The serum levels of TDCA, THDCA, TUDCA, TDCA/CA, and TDCA/DCA were significantly increased in the HFD group compared with those of the NC group, and kaempferol can decrease them. Furthermore, NASH mice had a higher liver level of total CA%, total CDCA%, primary BAs/secondary BAs, 12α-OH BAs/non-12α-OH Bas, and conjugated BAs/unconjugated BAs, and all decreased after kaempferol treatment. According to the RNA-seq results, we found that compared with the NC group, the mRNA expression of cholesterol-7α-hydroxylase (CYP7A1) in the HFD group was significantly increased, and the mRNA expression of sterol 12α‐hydroxylase (CYP8B1) and multidrug resistance-related protein 3 (MRP3) was significantly decreased, while kaempferol significantly promoted the mRNA expression of mitochondrial sterol 27-hydroxylase (CYP27A1) and Na+ -taurocholate cotransporting polypeptide (NTCP).Conclusion: βMCA, CA, UDCA, 12-DHCA, ωMCA, CDCA, TωMCA, TDCA, THDCA, TCDCA, and TUDCA in the serum, as well as 6,7-diketoLCA, 12-DHCA, and ωMCA in the liver, may be potential biomarkers for kaempferol to improve NASH. HFD-induced NASH may be associated with the increase of CYP7A1 and the decrease of CYP8B1, leading to increased BA synthesis, and the decrease of MRP3 leading to decreased BA synthesis, and kaempferol may alleviate NASH by increasing CYP27A1 and NTCP to enhance BA transport.

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“…It is the third most abundant protein in human bile after albumin and IgA (3). Unlike these two proteins, the biliary secretion of APF in rats is modulated by bile salts (4 APF is secreted by isolated hepatocytes, and its secretion is enhanced by bile salts (2). The potential physiological and pathophysiological properties of this biliary protein are only beginning to be explored.…”

Section: Tuvia Gilat~mentioning

confidence: 99%

Anionic polypeptide fraction in bile of patients with and without gallstones

Domingo

Lafont

Halpern³

et al. 1993

Hepatology

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With the demonstration of pronucleating and antinucleating proteins, the role of biliary proteins became of considerable research interest. Anionic polypeptide fraction is the third most abundant biliary protein; it is found in association with biliary lipids, has antinucleating properties for calcium and is found in gallstones. Its levels in various human biles have not been studied as of this writing. In this investigation the concentration of anionic polypeptide fraction in gallbladder bile was measured in 16 subjects without gallstones, 19 subjects with cholesterol stones and 15 subjects with pigment stones in Tel Aviv. Anionic polypeptide fraction concentrations in bile (mean +/- S.D.) were 0.76 +/- 0.09 gm/L in controls and 0.81 +/- 0.25 gm/L (which was not significant) in patients with cholesterol gallstones. They were significantly higher 1.03 +/- 0.23 (p < 0.05) in patients with pigment gallstones. The anionic polypeptide fraction/phospholipid ratio and the anionic polypeptide fraction/total lipid ratio were significantly higher in patients with pigment gallstones (p < 0.005 and 0.05, respectively). The anionic polypeptide fraction lipid ratios were insignificantly elevated in biles of patients with cholesterol stones compared with the ratios in biles of controls. Only the anionic polypeptide fraction/phospholipid ratio was significantly higher in biles of patients with pigment stones compared with those with cholesterol gallstones. The values were similar although higher in a small group of gallstone patients from Marseilles. The role of anionic polypeptide fraction in the pathogenesis of gallstones, particularly pigment gallstones, requires further study.

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Influence of Dehydrocholic and Cholic Acids on the Biliary Secretion of Anionic Polypeptide Fraction, the Major Apoprotein of the Biliary Lipoprotein Complex

Cited by 15 publications

References 26 publications

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Phosphatidylcholine-enriched diet prevents gallstone formation in mice susceptible to cholelithiasis

Kaempferol attenuates nonalcoholic steatohepatitis by regulating serum and liver bile acid metabolism

Anionic polypeptide fraction in bile of patients with and without gallstones

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