Influence of Deoxyribonucleic Acid on the Intermolecular Structure of Water

Depireux, J.; Williams, Dudley

doi:10.1038/195699b0

Cited by 20 publications

(2 citation statements)

References 9 publications

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“…I- 4 These studies were concerned with the width and shape of the HzO proton resonance peak in solutions of nucleic acids. There have also been structural studies of mononucleosides and mononucleotides by In this paper, we report initial studies on the high-resolution spectra of the nucleic acids themselves.…”

Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA

1964

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synopsisHigh resolution proton spectra in D20 solutions have been measured as functions of temperature for the following polynucleotides: Poly A, Poly U, Poly A and Poly U, Poly I, Poly C, Poly I and Poly C, and Transfer RNA from E. co2i (strain B), and from yeast. At denaturation, peaks characteristic of the mononucleotide protons appear. Areas under the peaks can be used as a direct measure of denaturation. The sharpness of the spectral lines indicates that in the denatured form internal rotation about the ribose-phosphate and the ribose-base links is relatively free. Linewidths also show that monomer units remain in either the ordered or freely rotating state for a t least lo-' see.

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Section: Introductionmentioning

confidence: 99%

Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA

1964

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“…Several investigators have suggested that substantial fractions of liquid water in biological samples are not observable by NMR methods (6, [13][14][15]. Subsequent investigations have not supported this hypothesis, although care must be exercised in the integration of the broad absorption lines which becomes more difficult as the width increases (1,4,7,8,11).…”

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confidence: 99%

Nuclear Magnetic Resonance of Water in Cold Acclimating Red Osier Dogwood Stem

1974

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The pulsed and continuous-wave nuclear magnetic resonance of water in cold-acclimating red osier dogwood (Cornus stolonilera Michx) stem showed reduced relaxation times and increased line width. The reduction of relaxation times suggests an over-all restriction in the motional characteristics of the water. The increased line width is not related to a molecular property of the water, but is useful in estimating the initiation of cold acclimation. Biphasic relaxation characteristics may be related to partitioning of the water at the cellular level. The liquid water content of the stem was a weak function of temperature between -25 and -55 C, corresponding to approximately 0.15 gram of water per gram of dry stem. The quantity of unfrozen water at subfreezing temperatures was not strongly dependent on the degree of cold acclimation. It is concluded that the ability of dogwood to survive low temperatures depends on its ability to tolerate diminished quantities of liquid water.Low temperature injury of plants is thought to result from intracellular ice formation or from freezing of extracellular water which may cause severe dehydration of the cells. Red osier dogwood (Cornus stolonifera Michx) has been studied previously and many details of the physiological alterations associated with acclimation to freezing stress and environmental factors which induce acclimation have been reported (10, 24). For example, changes in the quantities of water, lipids, carbohydrates, proteins, and nucleic acids usually occur (22,34). Although water has not been studied extensively as part of this problem, the status of water almost certainly is a vital factor in determining the resistance of living organisms to freezing stress. Nuclear magnetic resonance provides a particularly convenient tool for the study of water in plants because it is nondestructive and rich ' Scientific Journal Series Paper 8558 of the Minnesota Agricultural Experiment Station. in structural as well as dynamical information. NMR2 is used in two ways in the present study: to study the state of the unfrozen water in dogwood stems at low temperatures and to determine the amount of unfrozen water at low temperatures.NMR has been extensively used to study water in animal tissues such as muscle. In these samples, the NMR line is significantly broader than it is in pure water and the spin-spin relaxation time (T,2) is significantly shorter (2 to 6, 12, 13). These results are often interpreted to mean that cellular water consists of several populations of water molecule, including a bound fraction which experiences restricted motion. Some controversy has developed concerning the extent of the region of restricted water mobility and models range from a simplified two-phase model with a small fraction bound (2, 4, 5) to one consisting of all bound water (3,6,13

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On the hydration of DNA. I. Preferential hydration and stability of DNA in concentrated trifluoroacetate solution

Tunis

Hearst

1968

Biopolymers

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SynopsisThe hydration of DNA is an important factor in the stability of it.s secondary structure. Methods for measuring the hydration of DNA in solution and the results of various techniques are compared and discussed critically. The buoyant density of native and denatured T-7 bacteriophage DNA in potassium trifluoroacehte (KTFA) solution has been memured as a function of temperature between 5 and 50°C. The buoyant density of native DNA increased linearly with temperature, with a dependence of (2.3 f 0.5) X 10-4 g/cc-"C. DNA which has been heat denatured and quenched at 0°C in the salt solutiori shows a similar dependence of buoyant density on temperature a t temperatures far below the T,= and above the T,. However, there is an inflection region in the buoyant density versus 1' curve over a wide range of temperatures below the T,. Optical density versus temperature studies showed that this is due to the inhibition by KTFA of rccovcry of secondary structure on quenching. If the partial specific volume is assumed to be the same for native and denatured DNA, the loss of water of hydration on denaturation is calculated to be about 20% in KTFA at a water activity of 0.7 at 25°C. By treating the denaturation of DNA as a phase transition, an equation has been derived relating the destabilizing effect of trifluoroacetate to the loss of hydration on denaturation. The hydration of native DNA is abnormally high in the presence of this anion, and the loss of hydration on denaturation is greater than in CsCl. In addition, trifluoroacetate appears to decrease the A H of denaturation. 132sReceived February 1, 196s

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Influence of Deoxyribonucleic Acid on the Intermolecular Structure of Water

Cited by 20 publications

References 9 publications

Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA

Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA

Nuclear Magnetic Resonance of Water in Cold Acclimating Red Osier Dogwood Stem

On the hydration of DNA. I. Preferential hydration and stability of DNA in concentrated trifluoroacetate solution

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