Influence of diamines and polyamines on the senescence of plant suspension cultures

Muhitch, Michael J.; Edwards, L. A.; Fletcher, John S.

doi:10.1007/bf00270171

Cited by 19 publications

(6 citation statements)

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“…1998), development of flowers (Gerats et al. 1988) and fruits (Galston and Sawhney 1990), senescence (Muhitch et al. 1983), and pollen embryogenesis (Garrido et al.…”

Section: Introductionmentioning

confidence: 99%

“…processes, including rhizogenesis (Jarvis et al 1985) and root growth (Watson et al 1998), development of flowers (Gerats et al 1988) and fruits (Galston and Sawhney 1990), senescence (Muhitch et al 1983), and pollen embryogenesis (Garrido et al 1995). Changes in the cellular PA content in plant tissues have also been associated with various environmental factors.…”

Section: Introductionmentioning

confidence: 99%

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Up‐regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea)in response to stress

Mao

Pua²

2002

Physiologia Plantarum

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Arginine decarboxylase (ADC) is a key enzyme involved in the synthesis of polyamines, which have been implicated in a wide range of plant responses, including stress. However, regulation of polyamine levels in relation to ADC in response to stress at the molecular level is not well understood. In an attempt to address this question, we first cloned two cDNAs in mustard (Brassica juncea[L.] Czern & Coss var. Indian Mustard), designated MADC2 and MADC3, encoding predicted ADC. MADC2 and MADC3 encode polypeptides of 692 and 680 amino acid residues, respectively. A comparison of deduced amino acid sequence revealed that both were highly homologous to MADC1 (77%), a mustard ADC, and other plant ADCs (63-84%). Northern analysis revealed that ADC transcripts in mustard were generally more abundant in stem and root but were barely detectable in leaf. However, ADC expression in the leaf was up-regulated differentially in response to stress such as chilling, salt and mannitol and to treatments with exogenous polyamines. While chilling induced expression of all three ADC genes, salt predominantly resulted in increased accumulation of MADC3 transcript. Leaves exhibited a similar response to exogenous putrescine, spermidine and spermine, all of which stimulated accumulation of MADC2 and MADC3 transcripts but not MADC1. Furthermore, exogenous putrescine also increased the endogenous levels of spermidine and spermine, while a higher endogenous putrescine and spermidine content was detected in leaf incubated with exogenous spermine. Leaves also responded to chilling, salt and mannitol by increasing the levels of the cellular polyamine content, in which the level of spermine in free and conjugated forms increased most profoundly.

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“…1998), development of flowers (Gerats et al. 1988) and fruits (Galston and Sawhney 1990), senescence (Muhitch et al. 1983), and pollen embryogenesis (Garrido et al.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Up‐regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea)in response to stress

Mao

Pua²

2002

Physiologia Plantarum

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“…1985), development of flowers (Gerats et al . 1988) and fruits (Galston and Sawhney 1990), senescence (Muhitch et al . 1983) and plant morphogenesis in vitro (Galston et al .…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of S‐adenosyl‐methionine decarboxylase genes from mustard (Brassica juncea)

Gong

Pua

2005

Physiologia Plantarum

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is a key enzyme involved in the polyamine (PA) biosynthetic pathway. An understanding of how SAMDC genes are regulated is important for elucidating the molecular basis of PA biosynthesis and the role of PAs in plant growth and development. However, information regarding transcriptional regulation of SAMDC has been limited. In an attempt to address this question, we isolated four cDNAs from mustard (Brassica juncea), designated BJSAMDC1, BJSAMDC2, BJSAMDC3 and BJSAMDC4, encoding predicted SAMDC. A comparison of deduced amino acid sequence revealed that they are highly homologous to other plant SAMDCs. These proenzymes also possess the conserved cleavage domain and putative PEST sequence for SAMDC. Northern analysis showed that the SAMDC transcripts were most abundant in reproductive organs and roots but that the level was low in young leaves and petioles. Meanwhile, SAMDC expression in the leaf was up-regulated differentially in response to stress such as chilling and exogenous ACC. The effect of exogenous PAs on SAMDC expression appears to be divergent. While putrescine up-regulated the expression of BJSAMDC1, spermidine and spermine down-regulated its expression. Furthermore, mannitol was also shown to up-regulate SAMDC expression in a gene-specific manner, in which the BJSAMDC1 transcript increases but other SAMDC transcripts are not affected.

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“…Les polyamines semblent intervenir dans plusieurs processus physiologiques, tels que : action inhibitrice de la synthèse de l'éthylène (Apelbaum et al, 1981;Suttle, 1981), interaction avec les acides nucléiques (Bagni et al, 1981 ) et action anti-senescence (Altman, 1982; Muhitch et al, 1983; Kaur- Sawhney, 1987a). Des accumulations de putrescine ont été détectées dans des conditions physiologiques très diverses : en cas de déficience minérale (Smith, 1970;Basso et Smith, 1974), d'alimentation riche en azote ammoniacal (Le Rudulier, 1978), en réponse à un choc osmotique (Flores et ou à une diminution du pH du milieu nutritif (Flores et Tiburcio et al, 1986).…”

Section: Introductionunclassified

Évolution des polyamines dans les bourgeons et les rameaux de Pseudotsuga menziesii (Mirb) Franco à la suite du passage de l'état végétatif à l'état floral

Daoudi¹,

Bonnet-Masimbert²,

Martin-Tanguy

1991

Ann. For. Sci.

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(Pseudotsuga menziesii) after the transition from vegetative development to sexual development. After methanolic extraction and dansylation, polyamines (putrescina, spermidine, spermine) of shoots and buds of Douglas fir (Pseudotsuga menziesii) were separated using reverse phase high performance liquid chromatography (HPLC). They were quantified by fluorescence detection (fig 1). On plant material collected in fall, free polyamine levels were measured in morphologically distinct vegetative, male and female buds of ramets of one clone (1101) and in shoots (needles, stem, and buds) bearing only vegetative buds or vegetative as well as male and female buds of ramets of two clones (1101 and 1200). The distribution of polyamines was different between sexual and vegetative buds. Putrescine was the dominant polyamine in vegetative buds, while spermidine predominated in floral buds (fig 3). The highest concentration of spermidine was observed in male buds. Furthermore, spermine was only found in the sexual buds (fig 3). All polyamines also increased in the shoots bearing sexual buds (fig 4) compared to shoots with only vegetative buds. In general, the spermidine level was higher in buds than in shoots. Finally, the ratio between putrescine and spermidine in sexual buds as well as in shoots bearing these kinds of buds was much lower than in vegetative organs (tables / and II). The possibility of using these polyamine contents as physiological markers of sexual differentiation is discussed.

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Influence of diamines and polyamines on the senescence of plant suspension cultures

Cited by 19 publications

References 18 publications

Up‐regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea)in response to stress

Up‐regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea)in response to stress

Molecular cloning and characterization of S‐adenosyl‐methionine decarboxylase genes from mustard (Brassica juncea)

Évolution des polyamines dans les bourgeons et les rameaux de Pseudotsuga menziesii (Mirb) Franco à la suite du passage de l'état végétatif à l'état floral

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