Influence of diet on the distribution of nitrogen isotopes in animals

DeNiro, Michael J.; Epstein, Samuel

doi:10.1016/0016-7037(81)90244-1

Cited by 3,819 publications

(2,231 citation statements)

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“…Comparing the casein values with those of the feed, we observed an increase in the d 15 N values of about 2.8% for farm C and 2.1% for farm M. This situation is in agreement with what has been observed in meat protein, 39 even with less individual variability, 17 in cheese casein, 11 and in bulk milk. 25 The different 15 N enrichment observed in the two farms led to significantly ( p < 0.001) different d 15 N values of the relevant milk casein (C ¼ 5.6 AE 0.2%; M ¼ 4.6 AE 0.1%), which were not evident between the two overall diets.…”

Section: Isotopic Values Of Feedsupporting

confidence: 89%

“…It has been reported that the d 15 N of animal tissue is usually less influenced by specific dietary input and more variable due to metabolic factors. 1,39 Moreover, it has been stressed that the nutritional quality of dietary ingredients could affect diet-animal shifts: in general, better quality diets (i.e. with a lower C/N ratio) lead to a lower d 15 N enrichment.…”

Section: Isotopic Values Of Feedmentioning

confidence: 99%

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Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Camin

Perini

Colombari

et al. 2008

Rapid Comm Mass Spectrometry

123

134

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The stable isotope ratios ( 13 C/ 12 C, 15 Recently, several papers have been published about the application of stable isotope ratio analysis (SIRA) using isotopic ratio mass spectrometry (IRMS) to animal products. The aims of these studies were to obtain dietary reconstruction, to study animal movement patterns, 1-5 and to characterise the geographical origin. [6][7][8][9][10][11][12][13][14][15][16] Another important aim could be to study and quantify the alteration of stable isotope ratios (SIRs) in milk by substituting a C 3 plant diet with maize. This could be important in order to protect milk and milk derivatives produced using traditional or 'organic' practices of pasture compared with products produced with cheaper diets based on maize derivatives. There have been recent reports about this in defatted meat, [17][18][19] in related fat, 17 in several animal tissues such as blood, plasma, liver, kidney, cow hair 20 and in sheep faeces. 21 In these papers it was shown that there is a strong correlation between the SIR of carbon and the amount of maize in the diet. It was also shown that the degree of this alteration depends on the component or body tissue considered, due to its biochemical composition and turnover rate. 22 On the other hand, variations in the SIRs in milk and milk derivatives following suitable experimental design have not been so extensively explored. Metges et al. 23 investigated the 13 C/ 12 C of CO 2 breath, bulk milk and serum by exchanging a C 3 plant with a C 4 plant diet, and vice versa. Wilson et al. 24 studied the contribution of body protein to milk protein, changing abruptly from a C 3 to a C 4 diet. More recently, Knobbe et al. 25 followed the variation in stable carbon and nitrogen isotopic composition under different feeding regimes. It was concluded that, to obtain more detailed information on the influencing factors, the analysis of the SIRs of other elements such as oxygen and hydrogen may be necessary.In this study we considered around 130 cows stabled in two farms and supplied with diets made up of different kinds of C 3 plants and different amounts of maize. The different feed ingredients, the overall diet and the relevant milk (casein, lipid and some bulk samples) were subjected to analysis of 13

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Section: Isotopic Values Of Feedsupporting

confidence: 89%

Section: Isotopic Values Of Feedmentioning

confidence: 99%

Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Camin

Perini

Colombari

et al. 2008

Rapid Comm Mass Spectrometry

123

134

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“…Animal tissues are generally 15 N enriched relative to their diet (DeNiro and Epstein, 1981), the natural 15 N abundance (δ 15 N) being higher in body proteins than in the diet. We measured this difference, termed isotopic N fractionation (Δ 15 N animal-diet ), in different body pools (including rumen bacteria, duodenal digesta, feces and plasma proteins) under different nutritional conditions.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown across a variety of conditions that 15 N natural abundance (δ 15 N; 15 N/ 14 N ratio relative to atmospheric N 2 ) in animal proteins is higher than in the diet consumed (DeNiro and Epstein, 1981) and that variations in this isotopic N fractionation (Δ 15 N animal-diet = δ 15 N animal − δ 15 N diet ) are closely and negatively correlated with ENU in different species (Gaye-Siessegger et al, 2004;Cheng et al, 2013;Cantalapiedra-Hijar et al, 2015). The more efficiently the animals assimilate dietary N, the closer are the δ 15 N values in animal proteins and diet (i.e.…”

Section: Introductionmentioning

confidence: 99%

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?

Cantalapiedra-Hijar

Fouillet

Huneau

et al. 2016

animal

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Animal tissues are naturally 15 N enriched relative to their diet and the extent of this difference (Δ 15 N animal-diet ) has been correlated to the efficiency of N assimilation in different species. The rationale is that transamination and deamination enzymes, involved in amino acid metabolism are likely to preferentially convert amino groups containing 14 N over 15 N. However, in ruminants the contribution of rumen bacterial metabolism relative to animal tissues metabolism to naturally enrich animal proteins in terms of 15 N has been not assessed yet. The objective of this study was to assess the impact of rumen and digestion processes on the relationship between Δ 15 N animal-diet and efficiency of N utilization for milk protein yield (milk N efficiency (MNE); milk N yield/N intake) as well as the relationship between the 15 N natural abundance of rumen bacteria and the efficiency of N use at the rumen level. Solid-and liquid-associated rumen bacteria, duodenal digesta, feces and plasma proteins were obtained (n = 16) from four lactating Holstein cows fed four different diets formulated at two metabolizable protein supplies (80% v. 110% of protein requirements) crossed by two different dietary energy source (diets rich in starch v. fiber). We measured the isotopic N fractionation between animal and diet (Δ 15 N animal-diet ) in these different body pools. The Δ 15 N animal-diet was negatively correlated with MNE when measured in solid-associated rumen bacteria, duodenal digesta, feces and plasma proteins, with the strongest correlation found for the latter. However, our results showed a very weak 15 N enrichment of duodenal digesta (Δ 15 N duodenal digesta-diet mean value = 0.42) compared with that observed in plasma proteins (Δ 15 N plasma protein-diet mean value = 2.41). These data support the idea that most of the isotopic N fractionation observed in ruminant proteins (Δ 15 N plasma protein-diet ) has a metabolic origin with very little direct impact of the overall digestion process on the existing relationship between Δ 15 N plasma protein-diet and MNE. The 15 N natural abundance of rumen bacteria was not related to either rumen N efficiency (microbial N/available N) or digestive N efficiency (metabolizable protein supply/CP intake), but showing a modest positive correlation with rumen ammonia concentration. When using diets not exceeding recommended protein levels, the contribution of rumen bacteria and digestion to the isotopic N fractionation between animal proteins and diet is low. In our conditions, most of the isotopic N fractionation (Δ 15 N plasma protein-diet ) could have a metabolic origin, but more studies are warranted to confirm this point with different diets and approaches. ImplicationsThe difference in the natural abundance of 15 N between an animal and its diet (Δ 15 N animal-diet ) is a good biomarker for predicting the efficiency of N utilization. Our results in dairy cows fed diets not exceeding recommended protein levels demonstrate that digestion processes contribute little to the r...

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“…The δ 15 N values of animals have been used extensively to determine their trophic level based on the assumption of a considerable 15 N enrichment between each trophic level (DeNiro & Epstein 1981). The main reason for this trophic fractionation seems to be due to internal N metabolism, leading to 15 N enrichment in body proteins coupled with excretion of 15 N-depleted ammonia via urine (Balter et al 2006, Martínez del Rio et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Use of carbon and nitrogen stable isotopes to study the feeding ecology of small coastal cetacean populations in southern Brazil

et al. 2013

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of carbon and nitrogen stable isotopes to study the feeding ecology of small coastal cetacean populations in southern Brazil. Biota Neotrop. 13(4): http://www.biotaneotropica.org.br/v13n4/en/abstract?article+bn01413042013Abstract: Samples from individuals of the populations of Sotalia guianensis (Guiana) and Pontoporia blainvillei (Franciscana) dolphins living in the Babitonga Bay estuary (26° 28' S/48° 50' W), and samples from individuals of a second population of P. blainvillei from a nearshore area (26° 38' S/48° 41' W), were collected and analyzed along with their prey between 2000 and 2006, to determine the carbon and nitrogen stable isotope ratios and to verify differences in their feeding ecology. No differences were found in the median ð 15 N values of Guiana (15.2‰) and Franciscana (15.9‰) dolphins living in Babitonga Bay, nor of nearshore Franciscana (15.0‰) individuals, suggesting no variation in the trophic level of these populations. However, the lack of more information on the isotopic compositions of their putative prey in the nearshore areas prevents the ability to draw definitive conclusions on this issue. The estuarine Franciscana and Guiana dolphin populations presented mean ð 13 C values of approximately -15.7‰, which were not statistically different from nearshore Franciscana individuals (-14.8‰). Based on stomach content analyses of these species from a previous study, it was reported that there was little overlap in the diet of estuarine Franciscanas and Guiana dolphins. However, based on the similarity of the ð 13 C values between these two species and of their putative prey, it appears that in fact there is an overlap in the diet of these two species. Based solely on stable isotope analysis, it was not possible to differentiate between estuarine and nearshore Franciscana populations, making it difficult to conclude whether captured nearshore specimens were indeed yearlong residents of these areas. Finally, this study suggests that Franciscana and Guiana dolphin populations are sharing the same resources, mostly L. brevis, D. rhombeus, and S. rastrifer. Therefore, the combination of resource sharing and commercial exploitation of their prey makes these two cetacean species vulnerable. Keywords: Pontoporia blainvillei, Sotalia guianensis, Babitonga Bay, feeding ecology, trophic level.HARDT, F.A.S., CREMER, M.J., TONELLO JUNIOR, A.J., BELLANTE, A., BUFFA, G., BUSCAINO, G., MAZZOLA, S., BARRETO, A.S., MARTINELLI, L.A. & ZUPPI, G.M. O uso dos isótopos estáveis de carbono e nitrogênio para o estudo da ecologia de populações de pequenos cetáceos costeiros no sul do Brasil. Biota Neotrop. 13(4): http://www.biotaneotropica.org.br/v13n4/pt/abstract?article+bn01413042013Resumo: Amostras de tecido de indivíduos pertencentes às populações de Sotalia guianensis (boto-cinza) e Pontoporia blainvillei (toninha) que vivem no estuário da Baía da Babitonga (26° 28' S/48° 50' W) e de uma segunda população de P. blainvillei da área costeira (26° 38' S/48° 41' W) foram coletadas entre os anos 2000 e 2006 para ...

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Influence of diet on the distribution of nitrogen isotopes in animals

Cited by 3,819 publications

References 25 publications

Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?

Use of carbon and nitrogen stable isotopes to study the feeding ecology of small coastal cetacean populations in southern Brazil

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