Influence of Dietary Copper Proteinate on Performance, Selected Biochemical Parameters, Lipid Peroxidation, Liver, and Egg Copper Content in Laying Hens

Abstract

This study was performed to determine the effects of copper proteinate on performance, blood chemistry, lipid peroxidation status, and organs as well as copper deposition in the liver and eggs of laying hens. Seventy-two 30-week-old Bovans laying hens were distributed into four groups with three replicates. Animals were fed basal diet containing at least 17% crude protein and 2,800 kcal/kg metabolizable energy supplemented with either 0, 150, 300, or 450 mg/kg copper as copper proteinate. Supplementation of 150 and 300 mg/kg copper increased egg production, whereas 450 mg/kg copper decreased (p < 0.001). Liver copper levels were elevated in 300 and 450 mg/kg copper-supplemented groups (p < 0.001). Egg copper contents increased in all treatment groups (p < 0.01). An increase in glucose (p < 0.001) and decreases in albumin (p < 0.01) and total cholesterol (p < 0.05) levels were determined with 300 and 450 mg/kg copper. Supplementation of 450 mg/kg copper increased alkaline phosphatase and gamma glutamyl transpeptidase activities (p < 0.05), malondialdehyde, and high-density lipoprotein levels (p < 0.01) but decreased alanine aminotransferase and lactate dehydrogenase activities (p < 0.01). No gross and microscopic changes were observed in the liver and kidneys. These results indicated that 150 and 300 mg/kg copper increased egg production without having marked adverse effects, but 450 mg/kg copper altered some blood chemistry variables and reduced egg production in laying hens.

Effect of dietary copper addition on lipid metabolism in rabbits

“…In line with previous studies in ducks [17], laying hens [18], and broilers [34], copper treatment increased body weight gain and muscle tissue growth in rabbits. At the same time, copper treatment decreased liver and fat weight, suggesting an altered energy redistribution that favored lipolysis.…”

“…Dietary copper deficiency is associated with a variety of metabolic changes, including hypercholesterolemia, increased blood pressure, and glucose intolerance, in ducks [17], laying hens [18], and rodents [19,20]. The effect of copper on lipid metabolism has been disputed in previous studies.…”

Effect of yogurt containing deep sea water on health-related serum parameters and intestinal microbiota in mice

“…Our findings are consistent with the study of Minelli et al (2004), who reported that feeding yogurt to rats resulted in decreases in serum AST and ALT, whereas Yoshioka et al (2003) observed that DSW supplementation decreased serum ALT activity in rabbits receiving a diet containing 1% cholesterol. The effect of DSW could be caused by its microelement components, as evidenced by previous studies showing that in rats and laying hens, dietary supplementation with Zn and Cu, respectively, resulted in a reduction of serum AST and ALT (Kechrid and Bouzerna, 2004;Güçlü et al, 2008). Hokanson and Austin (1996) reported that blood TG level is a risk factor for cardiovascular disease independent of HDL-C level.…”

Effects of copper, iron, zinc, and manganese supplementation in a corn and soybean meal diet on the growth performance, meat quality, and immune responses of broiler chickens

“…Birds on the diet supplemented with 200 mg/kg of Fe reached lower ADG in the first phase of 21 d. However, trace mineral treatments with different levels of supplementation of the 4 minerals, which were close to the commercial recommendations for the minerals, did not show any further response in the growth performance of broilers in the current research. Supplementation of trace minerals at several fold the trace mineral levels suggested by the NRC [3] would lead to high levels of mineral excretion into the environment [5,7]. With the application of poultry manure, these elements have accumulated in the soil in some regions, and crop yields have been reduced because of high Cu or Zn levels [6,7].…”