Influence of dietary iron deficiency on hemoglobin, myoglobin, their respective reductases, and skeletal muscle mitochondrial respiration

Abstract

Male weanling rats were fed a control diet (46 ppm iron) or an iron-deficient diet (11 ppm iron) for 7 wk to determine the influence of iron deficiency on heme proteins and skeletal muscle mitochondrial respiration. At the end of 7 wk, the hemoglobin in the blood of the iron deficient rats was 35% less and skeletal muscle myoglobin was 20 to 37% less than in the control animals. The concentration of myoglobin in the heart was not appreciably diminished by iron deficiency. Cytochrome c concentration was 20% less in the heart and 35% less in the mixed-fiber gastrocnemius in the iron-deficient animals. Iron deficiency did not influence the activity of metmyoglobin reductase in either heart or skeletal muscle. There was about 30% more methemoglobin reductase activity in the red blood cells of the iron-deficient animals, which resulted in methemoglobin levels that were so low as to be virtually unmeasurable. In the iron-deficient rats, skeletal muscle mitochondrial respiration with either pyruvate-malate or palmitylcarnitine as substrate was 17 to 20% less than in the control animals. This study demonstrates that dietary iron deficiency of sufficient severity to reduce blood Hb and skeletal muscle myoglobin or cytochrome c also results in an impaired skeletal muscle oxidative capacity. The study also illustrates the preferential utilization of iron, not only between tissues, but within tissues, and tissue specific adaptive responses to iron deficiency.

The influence of iron deficiency on the functioning of skeletal muscles: experimental evidence and clinical implications

“…Importantly, ID is also accompanied by a diminished pool of myoglobin, an oxygen‐binding protein found in muscle tissue . The concentration of the aforementioned globin was decreased in predominantly slow‐ and mixed‐fibre skeletal muscle from iron‐deficient rats …”

Pharmacokinetics of Sodium Nitrite-Induced Methemoglobinemia in the Rat

“…Therefore, a Michaelis-Menten equation was used instead of an effective first-order rate constant for methemoglobin reductase activity. The maximal velocity of methemoglobin reductase was reported as 1.8 nmole/min/mg Hb (Hagler et al, 1981). Using 158 g Hb/liter blood gives a V max of 0.635 mM/min as heme groups.…”

Effects of dietary delta-aminolevulinic acid and vitamin C on growth performance, immune organ weight and ferrum status in broiler chicks

“…Saddoris et al (2003) reported that supplementation of the diet with Fe (Availa-Fe) resulted in a higher redness in the meat of pigs. Furthermore, Hagler et al (1981) suggested that the increased redness of muscle may be due to accumulation of myoglobin in response to improved Fe status. However, Apple et al (2000) suggested that minor improvements in pork color can be achieved by providing an additional 100 mg kg − 1 of Fe, which is in agreement with results of the current study.…”