Influence of Dietary n-3 Long Chain Polyunsaturated Fatty Acid Intake on Oxylipins in Erythrocytes of Women with Rheumatoid Arthritis

Abstract

Oxylipins derived from n-3 fatty acids are suggested as the link between these fatty acids and reduced inflammation. The aim of the present study was to explore the effect of a randomized controlled cross-over intervention on oxylipin patterns in erythrocytes. Twenty-three women with rheumatoid arthritis completed 2 × 11-weeks exchanging one cooked meal per day, 5 days a week, for a meal including 75 g blue mussels (source for n-3 fatty acids) or 75 g meat. Erythrocyte oxylipins were quantified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The results were analyzed with multivariate data analysis. Orthogonal projections to latent structures (OPLS) with effect projections and with discriminant analysis were performed to compare the two diets’ effects on oxylipins. Wilcoxon signed rank test was used to test pre and post values for each dietary period as well as post blue-mussel vs. post meat. The blue-mussel diet led to significant changes in a few oxylipins from the precursor fatty acids arachidonic acid and dihomo-ɣ-linolenic acid. Despite significant changes in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and free EPA in erythrocytes in the mussel group, no concurrent changes in their oxylipins were seen. Further research is needed to study the link between n-3 fatty-acid intake, blood oxylipins, and inflammation.

Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia

“…Four families of bioactive lipids are involved in inflammation and immune regulation: eicosanoids, specialised pro-resolving mediators, lysoglycerophospholipids/sphingolipids and endocannabinoids, which are generated from ω-3 or ω-6 polyunsaturated fatty acids (PUFAs). Eicosanoids are key elements in inflammation, and have been linked with diseases, such as viral infections, neurodegenerative disorders [2], rheumatoid arthritis [3], atherosclerosis [4,5], acute coronary syndrome [6], cancer [7,8], systemic lupus erythematosus [9], multiple sclerosis [10], liver injury [11], celiac disease [12], diabetes [13], cystic fibrosis [14], renovascular disease [15], asthma [16] and muscle dysfunction [17]. In addition, fatty acids, the common components of complex lipids, often act as immunomodulatory factors [18,19].…”

Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia

“…Four families of bioactive lipids are involved in inflammation and immune regulation; eicosanoids, specialized pro-resolving mediators, lysoglycerophospholipids/ sphingolipids and endocannabinoids, which are generated from ω-3 or ω-6 polyunsaturated fatty acids (PUFAs). Eicosanoids are key elements in inflammation, and have been linked with diseases, such as viral infections, neurodegenerative disorders [2], rheumatoid arthritis [3], atherosclerosis [4,5], acute coronary syndrome [6], cancer [7,8], systemic lupus erythematosus [9], multiple sclerosis [10], liver injury [11], celiac disease [12], diabetes [13], cystic fibrosis [14], renovascular disease [15], asthma [16] and muscle dysfunction [17]. In addition, fatty acids, the common components of complex lipids, often act as immunomodulatory factors [18,19].…”