Influence of Dietary Supplementation of Propolis and Bee Pollen on Liver Pathology in Broiler Chickens

Klarić, Ivana; Pavić, Mirela; Miškulin, Ivan; Blažičević, Valerija; Dumić, Albina; Miškulin, Maja

doi:10.3390/ani8040054

Cited by 15 publications

(16 citation statements)

References 22 publications

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“…Additionally, the hepatoprotective effects of these phytobiotics were suggested to be due to the presence of flavonoids in dandelion leaves and fenugreek seeds. Klaric et al’s [ 15 ] results also supported the results of the present study, where supplementation of phytobiotics such as propolis and bee pollen, which possess flavonoids, ameliorated the liver morphology compared to the control group. Additionally, normal hepatocytes without regressive lesions were noticed in the supplemented groups compared to the control.…”

Section: Discussionsupporting

confidence: 91%

“…Herbs have shown positive effects on the performance and biological health of broiler chickens [ 9 , 10 ], can improve haematological blood indicators and serum biochemical attributes [ 11 , 12 , 13 ], and have also been reported to regulate the kidney and liver functions [ 14 , 15 ]. Among such herbs, Persicaria odorata , of the family Polygonaceae, is of important significance.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes

Basit

Kadir

Loh

et al. 2020

Animals

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This research was conducted to estimate the effects of Persicaria odorata leaf meal (POLM) on haematological indices, serum biochemical attributes, and internal organs parameters, including histomorphological features of the liver, in broiler chickens. A total of 120 one-day-old male broiler chicks (Cobb-500) were randomly allocated into four experimental groups. The dietary treatments were basal diet (BD), which served as the control (C), along with BD + 2 g/kg POLM (Po2), BD + 4 g/kg POLM (Po4), BD + 8 g/kg POLM (Po8), which were the supplemented groups. The body weight gain (BWG) showed a linear increase and feed conversion ratio (FCR) showed a linear decrease with increasing POLM dosage at day 42 (p ˂ 0.05) and for the overall growth performance period (p ˂ 0.01). On day 21 and day 42, the values of red blood cells (RBCs), white blood cells (WBCs), haemoglobin (Hb), and packed cell volume (PCV) showed linear increases (p ˂0.05) as the dosage of POLM increased in the diet. On day 21, dietary supplementation of POLM linearly decreased (p ˂ 0.05) the serum activity of alkaline phosphatase (ALP), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), and serum levels of urea and creatinine. On the other hand, serum levels of total protein (TP), albumin, and globulin showed a linear increase (p ˂ 0.05) as the POLM dosage increased. On day 42, the serum activity of AST and ALT and serum levels of glucose, cholesterol, triglycerides, urea, and creatinine showed linear decreases (p ˂ 0.05) with increased levels of POLM in the diet. However, POLM supplementation linearly increased (p ˂ 0.05) the serum levels of TP and globulin. Dietary inclusion of POLM did not influence the organ parameters and showed no adverse effects on the liver histomorphology. In conclusion, supplementation of POLM increased the growth performance, improving haematological indices and serum biochemistry profiles of broiler chickens without any deleterious effects on the liver histomorphology. The results of the present study provide evidence that POLM can be safely used at a dose rate of 8 g/kg of feed as an alternative to conventional antimicrobial growth promoters (AGPs).

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes

Basit

Kadir

Loh

et al. 2020

Animals

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“…The botanical source of the CBM propolis are P. davidiana dode and P. simonii Carr, they are common distributed in the mountains area of northeast China [31,32]. There are many research about possibilities of propolis usage in the medicine or veterinary medicine [33]. Further research regarding the biological activity of CBM propolis and its compounds is planned.…”

Section: Resultsmentioning

confidence: 99%

A New Propolis Type from Changbai Mountains in North-east China: Chemical Composition, Botanical Origin and Biological Activity

et al. 2019

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Propolis is a bee product with a wide range of biological activities and its chemical compounds depend highly on the type of plant accessible to the bees. The Changbai Mountains are a major mountain range in Northeast China and are one of the major bee product-producing areas in China. In this study, we evaluated the total phenolic acids and flavonoid contents as well as the antioxidant activity of propolis sampled from the Changbai Mountains area (CBM). We identified the major compounds and qualified their contents by HPLC-ESI/MS and HPLC-UV, and found that the content of p-coumaric acid and an unknown peak (CBE) in CBM propolis was higher than in propolis from other parts of China. The unknown compound CBE was isolated, purified, and identified as benzyl p-coumarate by MS and NMR. Possible plant sources of CBM propolis are Populus davidiana dode and Populus simonii Carr, which widely distributed in the Changbai Mountains area. CBM propolis is a new propolis type, that could be an excellent raw material for health foods and pharmaceuticals.

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“…The present study revealed some original solutions regarding the applied dosage of investigated natural supplements and their specific combinations in broilers feeding, but was not without limitations. Due to the commonly accepted ’3Rs’, the authors had the justifiable wish to minimize the number of animals used in this study that had already been used in similar studies [3]. However, considering the tested natural feeding additives and main objective of this study, the authors believe that the described design of the study did not affect the results.…”

Section: Discussionmentioning

confidence: 99%

“…Propolis and bee pollen belong to a group of natural substances of animal and vegetable origin with intense antioxidant and antimicrobial properties [1]. The bioactive components of propolis and bee pollen include flavonoids, phenolic acids and their derivatives, which are also responsible for the bactericidal, antiviral, antifungal, analgesic, anti-inflammatory, antioxidant, immunostimulating and immunomodulating effects of these compounds in humans and animals [1,2,3].…”

Section: Introductionmentioning

confidence: 99%

Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

Prakatur

Miškulin

Pavić

et al. 2019

Animals

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The aim of this study was to determine the influence of dietary supplementation with propolis and bee pollen on the intestinal morphology and absorptive surface areas of chickens. Two hundred day-old Ross 308 chickens (100 male and 100 female) were equally allocated into five groups. Throughout the whole study, the control group of chickens was fed with a basal diet, while the experimental groups of chickens were fed with the same diet supplemented with propolis and bee pollen: P1 = 0.25 g of propolis/kg + 20 g of bee pollen/kg; P2 = 0.5 g of propolis/kg; P3 = 1.0 g of propolis/kg; P4 = 20 g of bee pollen/kg. The duodenal villi of chickens from all experimental groups were significantly higher and wider (p < 0.001), while their duodenal villi crypts were significantly deeper (p < 0.001) in comparison with these parameters in chickens from the control group. The villus height to crypt depth ratio, as well as the absorptive surface areas of broiler chickens, were significantly increased (p < 0.001) in experimental groups of chickens in comparison with the control group. These findings suggest that dietary supplementation with propolis and bee pollen has a beneficial effect on broilers chickens’ intestinal morphophysiology.

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Influence of Dietary Supplementation of Propolis and Bee Pollen on Liver Pathology in Broiler Chickens

Cited by 15 publications

References 22 publications

Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes

Effects of Inclusion of Different Doses of Persicaria odorata Leaf Meal (POLM) in Broiler Chicken Feed on Biochemical and Haematological Blood Indicators and Liver Histomorphological Changes

A New Propolis Type from Changbai Mountains in North-east China: Chemical Composition, Botanical Origin and Biological Activity

Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

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