Influence of different ammonium, lactate and glutamine concentrations on CCO cell growth

Abstract

In this study the effects of ammonium and lactate on a culture of channel catfish ovary (CCO) cells were examined. We also made investigation on the influence of glutamine, since our previous research revealed that this amino acid stimulated CCO cell growth more than glucose in a concentration-dependent manner. The effect of ammonium in cell culture included the considerable decrease in cell growth rate with eventual growth arrest as well as the retardation of glucose consumption. At ammonium concentrations above 2.5 mM, the cells displayed specific morphological changes. The effect of lactate was different to that of ammonium since the cell growth rate was progressively decreasing with the increase of lactate concentration, whereas the glucose consumption rate remained almost unchanged. Besides that, it was found that lactate was steadily eliminated from the culture medium when its initial concentration was relatively high. The influence of glutamine on CCO cell propagation showed that nutrient requirements of this cell line were mainly dependent on glutamine rather than glucose. The increase in glutamine concentration led to the increase in cell growth rate and consequent ammonia accumulation while the glucose utilization and lactate production were reduced. Without glutamine in culture medium cell growth was arrested. However, the lack of glucose reversed the stimulating effect of glutamine by decreasing cell growth rate and affecting amino acid utilization.

The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis

“…For production of cell culture-derived products this is a beneficial characteristic as higher ammonia concentrations are known to be toxic with negative impact on cell growth and product formation [22]. The observed ammonia concentration is at the lower limit compared to other cells, where concentrations between 3 and 8 mM were reported to lead to impaired growth [37-39]. …”

A Proliferation and surface marker characterization of adipose stem cells after culture in various processed outdated platelet lysate containing media

“…Without frequent medium change, the culture might lead to generation of metabolite/waste products, mainly lactate and ammonia that lead to decrease in cell growth rate. 24 Pham et al 25 reported that ADSC cultured in activated platelet with 20% CalCl 2 started to increase their proliferation rate after 3 days of culture and tend to rise to a maximum at day-7, and ADSC still growth until 11 days of culture with a lower trend in proliferation. In our study, hADSC started to show an increase in viable cell numbers at the beginning of culture, and reach a maximum at day-7 (F1, F2, F3 medium), while cells continued to grow at day-10 only on F2 medium.…”

Intracellular response to process optimization and impact on productivity and product aggregates for a high‐titer CHO cell process

“…It is widely known that high concentrations of ammonium, lactate, and osmolality can be detrimental to cell culture performance (Slivac, Blajić, Radošević, Kniewald, & Gaurina Srček, 2010;Zhu et al, 2005). It is widely known that high concentrations of ammonium, lactate, and osmolality can be detrimental to cell culture performance (Slivac, Blajić, Radošević, Kniewald, & Gaurina Srček, 2010;Zhu et al, 2005).…”