Influence of different chelators on the radiochemical properties of a 68-Gallium labelled bombesin analogue

Evaluating Ga-68 Peptide Conjugates for Targeting VPAC Receptors: Stability and Pharmacokinetics

“…There are various reported methods for testing stability. Asti et al reported stability (> 95 %) of [ 68 Ga]NODAGA, DOTA, and NOTA on incubation with mouse serum by UPLC method [32]. Persson et al have tested in vivo stability of [ 68 Ga]DOTA and DTPA-PA and found [ 68 Ga]DOTA-PA was 85 % stable and [ 68 Ga]DTPA-PA complex was 27 % stable at 4 h by HPLC method [33].…”

Evaluation of a PACAP Peptide Analogue Labeled with 68Ga Using Two Different Chelating Agents

“…The radiolabeling was evaluated by radio-HPLC. Ga-Transferrin (Tf) were prepared as described 25,26,13 and their retention times were recorded on radio-HPLC. of human serum solution.…”

“…They can also influence overall charge of the complex biodistribution and more importantly uptake in the tumor site. [11][12][13] In recent years, there has been increasing interest in the receptor targeted scintigraphy in which peptides have emerged as promising biomolecules.…”

“…Chelator NODAGA has flexibility over the influence of temperature and acidic environment. 13 Labeling at room temperature provides an advantage and could prevent any deleterious effect on peptide conjugate due to heating. Both peptide conjugates were found to be stable in serum against transchelation and transmetallation experimentations.…”

Preparation and evaluation of a single vial AMBA kit for 68Ga labeling with potential for imaging of GRP receptor-positive cancers

“…Efficacy of both 68 Ga-AMBA and 177 Lu-AMBA preparations for their intended use has been demonstrated in pre-clinical studies as well as in human volunteers [31,32]. Recently, Asti et al have studied the influence of various chelators viz., DOTA, NOTA and NODAGA on the radiochemical properties of AMBA [33] and Liu et al have reported on the preparation and bioevaluation of 64 Cu and 18 F labeled NODAGA-AMBA [34]. Hence, while there are reports on the automated synthesis of 68 Ga-AMBA using the commercially available 68 Ge/ 68 Ga generators and the AMBA kits originally formulated for preparation of 177 Lu-AMBA [28,35], to the best of our knowledge, a straight-forward kit formulation strategy exclusively for radiolabeling with 68 Ga has not been reported elsewhere.…”