Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Abstract

Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search for factors and influences for the intensification of this process. In this case, it is very promising to use the precursors of their biosynthesis as potential “regulators” of the various metabolites’ formation. The purpose of our research was to study the effect of L-phenylalanine (PhA, 3 mM), trans-cinnamic acid (CA, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on accumulation of various phenolic compound classes, including phenylpropanoids, flavans and proanthocyanidins, as well as the content of malondialdehyde in in vitro callus culture of the tea plant (Camellia sinensis L.). According to the data obtained, the precursors’ influence did not lead to changes in the morphology and water content of the cultures. At the same time, an increase in the total content of phenolic compounds, as well as phenylpropanoids, flavans and proanthocyanidins, was noted in tea callus cultures. Effectiveness of precursor action depends on its characteristics and the exposure duration, and was more pronounced in the treatments with PhA. This compound can be considered as the most effective precursor regulating phenolic metabolism, contributing to a twofold increase in the total content of phenolic compounds, flavanes and proanthocyanidins, and a fourfold increase in phenylpropanoids in tea callus cultures.

Evaluation of Total Isoflavones in Chickpea (Cicer arietinum L.) Sprouts Germinated under Precursors (p-Coumaric Acid and L-Phenylalanine) Supplementation

“…They are precursors of a variety of metabolites that regulate plant growth, metabolism, and interactions with environmental stimuli, such as biotic and abiotic stresses [14][15][16][17][18][19]. They have also been utilized in food supplements, pharmaceuticals, and cosmetics, and have numerous advantages for human well-being [20,21]. Chickpea seeds and sprouts contain diverse phenylpropanoids that contribute to their immense antioxidant, estrogenic, and antiproliferative activities [22].…”

Phenolic compound profiling and antioxidant potential of different types of Schisandra henryi in vitro cultures

“…Different precursors were added to increase the accumulation of secondary metabolites. The research revealed the presence of polyphenolic compounds from the group of phenylpropanoids, flavans, and proanthocyanidins and confirmed that the addition of precursors such as phenylalanine significantly increased the accumulation of the compounds (Aksenova et al 2023 ).…”

“…A large number of publications are based on research on the polyphenol profile of extracts from different types of in vitro cultures. The authors focus on the qualitative and quantitative determination of polyphenols, also examining antioxidant potential (Aksenova et al 2023 ; Zheleznichenko et al 2023 ; Kwiecień et al 2023 ).…”