Influence of direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet1

Giraldo, L. A.; Tejido, M. L.; Ranilla, María José; Ramos, S.; Carro, M. D.

doi:10.2527/jas.2007-0343

Cited by 83 publications

(71 citation statements)

References 30 publications

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“…Similar results were reported for alfalfa hay fed to sheep where a 6.3% increase on dry matter digestibility and 11.7% more hemicellulose digestibility (Pinos-Rodríguez et al 2002). However, addition of commercial fibrolytic enzymes, mainly endoglucanase and xylanase directly fed into sheep's rumen at a rate of 0 or 12 g d -1 , did not correlate with in situ dry matter and NDF degradation of grass hay (Giraldo et al 2008). A synergistic action between rumen and exogenous fibrolytic enzymes being active at physiological digestive conditions has been reported (Morgavi et al 2000).…”

Section: In Vitro Ruminal Digestibility and True Digestibility Of Cssupporting

confidence: 72%

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Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Regalado‐González¹,

Vázquez-Obregón²,

García-Almendárez³

et al. 2011

Electro Journal of Biotech

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Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2 9-5 , and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a DraperLin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein) -1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg -1 dry ACS for 12 hrs before exposure to cow's ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed.

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Section: In Vitro Ruminal Digestibility and True Digestibility Of Cssupporting

confidence: 72%

“…Xylanase extracts may be incorporated as ruminant feed additives, to improve ruminal fiber digestion resulting in increased digestible energy intake (Pinos-Rodríguez et al 2002;Giraldo et al 2008). Furthermore, the demand for xylo-oligosaccharides has increased due to their prebiotic function (Maalej-Achouri et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Regalado‐González¹,

Vázquez-Obregón²,

García-Almendárez³

et al. 2011

Electro Journal of Biotech

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“…Hvelplund et al (2009) found that use of aerobic enzymes in combination with ensiling showed negative effects as the Neutral Detergent Fiber (NDF) content increased and the in vitro digestibility decreased markedly (between 14 and 19% units) compared to the silage without enzyme treatment and irrespective of forage. Another study by Giraldo et al (2008) demonstrated that supplementing a fibrolytic enzyme directly into the rumen increased the fibrolytic activity in ruminal fluid without a pre-feeding feed-enzyme interaction. Although enzyme supplementation did not affect significantly the concentration of total Volatile Fatty Acid (VFA) in ruminal fluid, molar proportions of propionate increased and acetate: propionate ratio decreased, which might indicate a change in ruminal bacterial populations.…”

Section: Application Of Enzymesmentioning

confidence: 99%

Exogenous Enzymes in Ruminant Nutrition: A Review

Sujani¹,

Seresinhe²

2015

Asian J. of Animal Sciences

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The concept of supplementation of ruminant diets with exogenous enzymes is not new though a considerable research interest on this field has been emerged from 1990s. Availability of wide range of exogenous enzyme products, development of better methods to evaluate enzyme activity, revised knowledge on rumen functions and recent advances of biotechnology which lowers the cost of enzyme production have fuelled the enzyme utilization in ruminant industries. The exogenous enzymes used in ruminant diets can be characterized in to main categories as fibrolytic, amylolytic and proteolytic based on specific substrate on which their enzyme activity can perform. The sources of these exogenous enzymes are mainly four bacterial species, three fungal species and some yeasts. Solid State Fermentation (SSF) and Submerged Fermentation (SmF) are the major methods for enzyme extraction which have combined with several other biotechnological aspects. The enzyme application method can vary in a wide range from applying to forage at harvesting, at ensiling, at feeding and the portion of feed which is mixed with enzyme may also vary from to forage to concentrate to a part of totally mixed rations. Up to date numerous number of researches have been done with ruminants like cattle both dairy and beef, goat, sheep and less with buffalo. The results seems to be inconsistent but positive results on feed intake, nutrient digestibility, growth performance, other production parameters, manure nutrient excretion etc., have been obtained. Therefore more fine-tuned research efforts are highly suggested for the generalization of exogenous enzyme usage in ruminant nutrition.

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“…Similarly, Nsereko et al (2002) found that EFE indirectly increased the attachment and the number of cellobiose-and glucose-utilizing bacteria in the rumen. Giraldo et al (2008) reported that treating high-forage diets with EFE increased fibrolytic activity and stimulated the in vitro numbers of micro-organisms. Likewise, Yang et al (1999) found that EFE significantly improved the supply of metabolizable protein to the small intestine, measured as 15 N. In two other studies evaluating this enzyme at a similar dose, it improved (P <0.05) body weight gains and feed conversion efficiency when fattening lambs on forage based-diets (Cruywagen & Goosen, 2004;Cruywagen & Van Zyl, 2008).…”

Section: Resultsmentioning

confidence: 99%

Digestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymes

Vyver¹,

Useni²

2012

SA J. An. Sci.

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_______________________________________________________________________________ AbstractThe aim of the study was to determine the effect of an exogenous fibrolytic enzyme (EFE) on the ruminal disappearance of dry matter (DM), neutral detergent fibre (NDF), crude protein (CP) and on the microbial protein synthesis (MPS) in sheep of a milled substrate consisting of a 50 : 50 mixture of lucerne hay (LH) and wheat straw (WS). Four cannulated Döhne-Merino sheep were randomly assigned to two groups in a 2 x 2 cross-over experiment. Additional to the in situ study, the effects of an exogenous fibrolytic enzyme (EFE) on the in vitro gas production (GP) and ANKOM digestion systems on the mixture of milled LH and WS were determined. The substrate was pre-treated with distilled water (control) or EFE (treatment) 12 hours prior to incubation to allow interaction between the substrate and enzyme. Adding EFE significantly improved the in vitro cumulative gas production (GP), but it did not affect the in vitro MPS measured as purine derivates of the GP residues. The enzyme significantly increased the in vitro DM and NDF disappearances after 36 hours. However, the in situ disappearances of DM, NDF and CP of the roughage mixture were not affected by the EFE. Further, the in situ MPS was increased significantly with the EFE treatment of the mixed substrate. It is concluded that the improved ruminal digestion of DM, NDF and CP is not only due to direct enzyme hydrolysis, but also due to the improvement of MPS.________________________________________________________________________________

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Influence of direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet1

Cited by 83 publications

References 30 publications

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility

Exogenous Enzymes in Ruminant Nutrition: A Review

Digestion and microbial protein synthesis in sheep as affected by exogenous fibrolytic enzymes

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