Influence of dissimilatory metal reduction on fate of organic and metal contaminants in the subsurface

Lovley, Derek R.; Anderson, Robert

doi:10.1007/pl00010974

Cited by 155 publications

(79 citation statements)

References 73 publications

(65 reference statements)

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“…Direct microbial (enzymatic) reduction coupled to oxidation of organic carbon and H 2 is recognized as the dominant mechanism for Fe(III) oxide reduction in nonsulfidogenic anaerobic soils and sediments (see Lovley 1991Lovley , 2000. This process contributes to both natural and contaminant (hydrocarbon) organic carbon oxidation in sedimentary environments, and exerts a broad range of impacts on the behavior of trace and contaminant metals and radionuclides (Lovley and Anderson 2000). Reduced sulfur (S) oxidation processes (both biotic and abiotic) drive much of Fe(III) oxide reduction in sulfur-rich marine sediments (e.g.…”

Section: Bacterial Fe Redox Cyclingmentioning

confidence: 99%

Potential for Microscale Bacterial Fe Redox Cycling at the Aerobic-Anaerobic Interface

Roden

Sobolev

Glazer

et al. 2004

Geomicrobiology Journal

137

105

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Section: Bacterial Fe Redox Cyclingmentioning

confidence: 99%

Potential for Microscale Bacterial Fe Redox Cycling at the Aerobic-Anaerobic Interface

Roden

Sobolev

Glazer

et al. 2004

Geomicrobiology Journal

137

105

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“…The process has been extensively studied (Geomicrobiology, 2002;DiChristina et al, 2005;Kappler and Straub, 2005;Roden, 2006;Weber et al, 2006). Dissimilatory Fe(III) reduction influences system redox state and buffering capacity Komlos et al, 2007); the valence and composition of pore-water solutes Bennett et al, 1993); the identity, surface properties, and geochemical reactivity of the Fe-containing mineral fraction (e.g., Zachara et al, 2004;Plymale et al, 2011); and the chemical form, persistence, and fate of a wide range of inorganic and organic contaminants (Lovley and Anderson, 2000).…”

Section: Introductionmentioning

confidence: 99%

The mineralogic transformation of ferrihydrite induced by heterogeneous reaction with bioreduced anthraquinone disulfonate (AQDS) and the role of phosphate

Zachara¹,

Kukkadapu²,

Peretyazhko³

et al. 2011

Geochimica et Cosmochimica Acta

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Bruce, "The mineralogic transformation of ferrihydrite induced by heterogeneous reaction with bioreduced anthraquinone disulfonate (AQDS) and the role of phosphate" (2011 Abstract Bioreduced anthraquinone-2,6-disulfonate (AH 2 DS; dihydro-anthraquinone) was reacted with a 2-line, Si-substituted ferrihydrite under anoxic conditions at neutral pH in PIPES buffer. Phosphate (P) and bicarbonate (C); common adsorptive oxyanions and media/buffer components known to effect ferrihydrite mineralization; and Fe(II) aq (as a catalytic mineralization agent) were used in comparative experiments. Heterogeneous AH 2 DS oxidation coupled with Fe(III) reduction occurred within 0.13-1 day, with mineralogic transformation occurring thereafter. The product suite included lepidocrocite, goethite, and/or magnetite, with proportions varing with reductant:oxidant ratio (r:o) and the presence of P or C. Lepidocrocite was the primary product at low r:o in the absence of P or C, with evidence for multiple formation pathways. Phosphate inhibited reductive recrystallization, while C promoted goethite formation. Stoichiometric magnetite was the sole product at higher r:o in the absence and presence of P. Lepidocrocite was the primary mineralization product in the Fe(II) aq system, with magnetite observed at near equal amounts when Fe(II) was high [Fe(II)/Fe(III)] = 0.5 and P was absent. P had a greater effect on reductive mineralization in the Fe(II) aq system, while AQDS was more effective than Fe(II) aq in promoting magnetite formation. The mineral products of the direct AH 2 DS-driven reductive reaction are different from those observed in AH 2 DS-ferrihydite systems with metal reducing bacteria, particularly in presence of P. Ó

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“…Detailed studies of their metabolism have revealed them to be capable of bioremediation of several heavy metals, including uranium, plutonium, technetium, and vanadium, as well as biodegradation of several organic contaminants, including monoaromatic hydrocarbons (16,17,26). More recently, Geobacter species have been used to generate electricity from waste organic matter (2,15,19). These unique metabolisms make Geobacter species important players in the contaminated subsurface environment (18).…”

mentioning

confidence: 99%

Flux Analysis of Central Metabolic Pathways in Geobacter metallireducens during Reduction of Soluble Fe(III)-Nitrilotriacetic Acid

Tang

Chakraborty

Martín

et al. 2007

Appl Environ Microbiol

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We analyzed the carbon fluxes in the central metabolism of Geobacter metallireducens strain GS-15 using 13 C isotopomer modeling. Acetate labeled in the first or second position was the sole carbon source, and Fenitrilotriacetic acid was the sole terminal electron acceptor. The measured labeled acetate uptake rate was 21 mmol/g (dry weight)/h in the exponential growth phase. The resulting isotope labeling pattern of amino acids allowed an accurate determination of the in vivo global metabolic reaction rates (fluxes) through the central metabolic pathways using a computational isotopomer model. The tracer experiments showed that G. metallireducens contained complete biosynthesis pathways for essential metabolism, and this strain might also have an unusual isoleucine biosynthesis route (using acetyl coenzyme A and pyruvate as the precursors). The model indicated that over 90% of the acetate was completely oxidized to CO 2 via a complete tricarboxylic acid cycle while reducing iron. Pyruvate carboxylase and phosphoenolpyruvate (PEP) carboxykinase were present under these conditions, but enzymes in the glyoxylate shunt and malic enzyme were absent. Gluconeogenesis and the pentose phosphate pathway were mainly employed for biosynthesis and accounted for less than 3% of total carbon consumption. The model also indicated surprisingly high reversibility in the reaction between oxoglutarate and succinate. This step operates close to the thermodynamic equilibrium, possibly because succinate is synthesized via a transferase reaction, and the conversion of oxoglutarate to succinate is a rate-limiting step for carbon metabolism. These findings enable a better understanding of the relationship between genome annotation and extant metabolic pathways in G. metallireducens.Geobacter species are known to be one of the dominant groups of microorganisms mediating iron reduction in the environment (21). They have been found to be ubiquitous in a myriad of subsurface environments. Detailed studies of their metabolism have revealed them to be capable of bioremediation of several heavy metals, including uranium, plutonium, technetium, and vanadium, as well as biodegradation of several organic contaminants, including monoaromatic hydrocarbons (16,17,26). More recently, Geobacter species have been used to generate electricity from waste organic matter (2, 15, 19). These unique metabolisms make Geobacter species important players in the contaminated subsurface environment (18). Geobacter metallireducens was the first iron-reducing organism isolated that coupled complete oxidation of organic acids with reduction of iron oxides (20,21). It completely oxidizes organic carbons such as fatty acids, alcohols, and monoaromatic compounds via the tricarboxylic acid (TCA) cycle (3, 20) coupled with the reduction of iron. The genomes of several Geobacter species have been sequenced, and proteome data are also available (5, 24). While the genome sequence and proteome are important for understanding Geobacter, they are not necessarily accurate represe...

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Influence of dissimilatory metal reduction on fate of organic and metal contaminants in the subsurface

Cited by 155 publications

References 73 publications

Potential for Microscale Bacterial Fe Redox Cycling at the Aerobic-Anaerobic Interface

Potential for Microscale Bacterial Fe Redox Cycling at the Aerobic-Anaerobic Interface

The mineralogic transformation of ferrihydrite induced by heterogeneous reaction with bioreduced anthraquinone disulfonate (AQDS) and the role of phosphate

Flux Analysis of Central Metabolic Pathways in Geobacter metallireducens during Reduction of Soluble Fe(III)-Nitrilotriacetic Acid

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