Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.)

Assani, Akym; Haïcour, R.; Wenzel, G.; Foroughi‐Wehr, B.; Bakry, Frédéric; Côte, François-Xavier; Ducreux, G.; Ambroise, Annick; Grapin, Agnès

doi:10.1016/s0168-9452(01)00562-3

Cited by 42 publications

(33 citation statements)

References 24 publications

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“…The transfer of germinated embryos into medium containing 4.4 mM BA and 5.4 mM NAA resulted in formation of plantlets (Fki et al 2003). A similar result was obtained in banana somatic embryos (Assani et al 2001(Assani et al , 2002.…”

Section: Concluding Remarks On Cell Suspension Culturesupporting

confidence: 82%

“…The combination of cellulase RS-pectolyase-hemicellulase gave a higher number of protoplasts in our investigation. Using the same approach in banana protoplast isolation (Assani et al 2002), the mixture of cellulase-pectolyase-hemicellulase was more efficient for protoplast isolation from cell suspension after 15-17 h in darkness. The protoplast isolation involved the treatment of tobacco leaves with pectinase to separate the cells, followed by cellulase to remove their wall (Takebe et al 1968).…”

Section: Regeneration Of Protoplastsmentioning

confidence: 99%

“…Previous investigations showed that the impact of genotype on plant regeneration from protoplasts in apple and banana is significant (Assani et al 2002;Patat-Ochatt and Lespinasse 1993). Currently, protoplasts can be isolated from almost all plant tissues, calli and cell suspensions; however, the type of the donor material is crucial for shoot organogenesis and somatic embryogenesis.…”

Section: Regeneration Of Protoplastsmentioning

confidence: 99%

See 2 more Smart Citations

Date Palm Cell and Protoplast Culture

Assani

Chabane

Shittu

et al. 2011

Date Palm Biotechnology

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This chapter describes the current status of cell and protoplast cultures in date palm (Phoenix dactylifera L.). Critically important steps toward plant regeneration from recalcitrant date palm protoplasts have been achieved in the recent past. Callus regeneration was achieved in commercial cvs. Deglet Noor, Takerboucht, Barhee and Zaghloul. The use of feeder layer was the main factor for inducing cell divisions as well as subsequent microcallus and callus formation. Presently, a protoplast-to-plant system has been reported for more than 400 species, of which the family Solanaceae is predominantly represented. In crops like potato, tobacco, brassica, citrus and eggplant seed companies are using protoplast fusion based technology to produce new commercial varieties. In the last decade however, little progress has been made regarding protoplast regeneration from the so-called recalcitrant species which includes date palm. There are certain similarities between some protoplast systems and mammalian stem cells. More collaboration between animal and plant scientists is highly encouraged, which would be useful for both parties to help address these challenges.

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Section: Concluding Remarks On Cell Suspension Culturesupporting

confidence: 82%

Section: Regeneration Of Protoplastsmentioning

confidence: 99%

Section: Regeneration Of Protoplastsmentioning

confidence: 99%

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Date Palm Cell and Protoplast Culture

Assani

Chabane

Shittu

et al. 2011

Date Palm Biotechnology

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“…At 5-6 months of culture, 20% of the explants produced embryogenic calli (Figure 1 B). Different authors have reported that somatic embryogenesis is genotype dependent (Assani et al, 2002;Gahan & George, 2008). However, the embryogenic frequency in banana was not only dependent on the genome group, but it also varied with the variety within genome groups and even from one experiment to another (Strosse et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

Morais-Lino

Santos-Serejo

Silva

et al. 2008

Pesq. agropec. bras.

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-The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

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“…Depending on the genotype and cell suspension quality, the protocol allows one to obtain: -a variable protoplast yield: approx [10 7 to (3 × 10 7 )] protoplasts per mL PCV cell suspension [9], -protoplasts with size varying between (10 and 35) µm, -protoplasts with 70-90% viability when just isolated, -approximately 70-80% of wall reformation 6-8 days after protoplast isolation, -approximately 0.2% of plated protoplasts producing embryos, 1.5-2.5 months after protoplast plating, -plant regeneration from 45-65% of embryos, 3-4 months after protoplast plating.…”

Section: Expected Resultsmentioning

confidence: 99%

Protoplast isolation and culture for banana regeneration via somatic embryogenesis

Haïcour

Assani

BuiTrang³

et al. 2009

Fruits

Self Cite

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Fruits, vol. 64 (4) 261Protoplast isolation and culture for banana regeneration via somatic embryogenesis.Abstract --Introduction. This protocol describes a method for obtaining protoplasts from banana leaves, calli and cell suspensions, and their sustainable development via somatic embryogenesis from embryogenic cell suspensions. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required laboratory materials, and media preparation for protoplast production and culture. Results. The first protoplasts may be seen after 30 min of incubation in enzyme maceration. With protoplasts from embryogenic cell suspension, complete development into a whole plant, through somatic embryogenesis, is observed in 12 weeks. The first cell divisions occur on feeder layers 3-8 days after protoplast plating. Proembryo formation is observed 14-21 days after initiation of protoplast culture. The transfer of derived embryo plantlets, at 8-10 weeks after protoplast plating, onto growth regulator-free medium, leads to plant rooting and elongation. Résumé --Introduction. Le protocole décrit une méthode qui permet d'obtenir des protoplastes à partir de feuilles, de cals et de suspensions cellulaires de bananiers, ainsi que le développement, par embryogenèse somatique, des protoplastes issus de suspensions cellulaires embryogènes. Le principe, les principaux avantages de la méthode, le matériel végétal de départ, le temps nécessaire et les résultats attendus sont présentés. Matériel et méthodes. Cette partie décrit le matériel de laboratoire nécessaire, la préparation des milieux pour l'obtention de protoplastes, ainsi que leur culture. Résultats. Dès la première demi-heure d'incubation dans la solution enzymatique, les premiers protoplastes sont libérés. En 12 semaines, les protoplastes, issus de suspensions cellulaires embryogènes, se développent en plantes entières via l'embryogenèse somatique. Sur les couches nourricières, les protoplastes reconstituent leur paroi ; les premières divisions cellulaires s'observent (3 à 8) jours après l'étalement des protoplastes. La formation des proembryons se déroule entre (14 et 21) jours après le début des cultures de protoplastes. Puis, (8 à 10) semaines après l'étalement des protoplastes, leur évolution embryogène conduit à des plantules qui s'enracinent et s'allongent après transfert sur un milieu dépourvu de facteurs de croissance.France / Musa sp. / méthode / milieu de culture / technique de culture / protoplaste / embryogenèse somatique / régénération in vitro

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Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.)

Cited by 42 publications

References 24 publications

Date Palm Cell and Protoplast Culture

Date Palm Cell and Protoplast Culture

Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

Protoplast isolation and culture for banana regeneration via somatic embryogenesis

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