Influence of Dose Uniformity when Replicating a Gamma Knife Mouse Model of Radiation Necrosis with a Preclinical Irradiator

Boria, Andrew J.; Pérez-Torres, Carlos J.

doi:10.1667/rr15273.1

Cited by 8 publications

(26 citation statements)

References 26 publications

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“…The goal of this study was to determine the effect mouse strain and sex in reference to our previously established model of RN. We analyzed all mice from four to eight weeks post-irradiation as we had previously shown that the former is the onset of lesion and the latter is the peak lesion size on BALB/cN females [3,4]. We evaluated the induction of radiation necrosis using three metrics: T1-weighted MRI, T2-weighted MRI and a histology score.…”

Section: Resultsmentioning

confidence: 99%

“…The rationale was that these two parameters are known to not be consistent in prior reports of murine models of radiation necrosis. Our prior publications have already shown that radiation delivery parameters (dose, device, and fractionation) can all affect the results [3,4]. Additional biological factors not included in this report but also of interest would be age at irradiation and species, as rats have also been used to model radiation necrosis [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…However, biological parameters can also influence the ability to properly reproduce the model. There are examples in the literature of brain irradiations being modeled in BALB/c [3,4] and C57BL/6 [5,6], which is not surprising as they are the two most common "normal" mouse strains. Ironically, for the cited reports above, the BALB/c studies were done in females while the C57BL/6 studies were done in males which is another biological factor that could influence the response of the model.…”

Section: Introductionmentioning

confidence: 99%

“…The goal of this study was to determine the effect mouse strain and sex have in reference to our previously established model of RN [3,4]. As both the BALB/c and C57BL/6 strains have existed for over 100 years, and genetic drift is known to have occurred [10,11], we further decided to include two different vendors for each strain encompassing the N and J substrains for each strain.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Mouse Strain and Sex when Modeling Radiation Necrosis

Boria

Pérez-Torres

2020

Preprint

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Background: Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the train and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis. Methods: We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each(BALB/cN, BALB/cJ, C57BL/6N, and C57BL/6J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to eight weeks with a 7T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0-3 in ascending order of damage. Results: Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development. Conclusion: Mouse strain thus need to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of Mouse Strain and Sex when Modeling Radiation Necrosis

Boria

Pérez-Torres

2020

Preprint

Self Cite

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show abstract

“…The goal of this study was to determine the effect mouse strain and sex have in reference to our previously established model of RN [3,4]. As both the BALB/c and C57BL/6 strains have existed for over 100 years, and genetic drift is known to have occurred [9,10], we further decided to include two different vendors for each strain encompassing the N and J substrains for each strain.…”

mentioning

confidence: 99%

Impact of Mouse Strain and Sex when Modeling Radiation Necrosis

Boria

Pérez-Torres

2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the train and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis.Methods: We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each(BALB/cN, BALB/cJ, C57BL/6N, and C57BL/6J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to eight weeks with a 7T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0-3 in ascending order of damage.Results: Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development.Conclusion: Mouse strain thus need to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration.

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