Influence of Doxycycline and InGaAlP Diode Laser at 660 nm Wavelength in the Treatment of Periodontitis Induced in Rats: <i>In Vivo</i> Study

Silveira, Glauco Rodrigues Carmo; Lima, Daniela Coêlho de; Cintra, Luciano Tavares Ângelo; Brigagão, Maı́sa Ribeiro Pereira Lima; Ervolino, Edílson; Fernandes, Leandro Araújo

doi:10.1111/php.13414

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Astuti et al used doxycycline-assisted photodynamic therapy in the gingival sulcus of mice with periodontitis and successfully eliminated Porphyromonas gingivalis bacteria, resulting in effective improvement (16). A similar improvement by using laser radiation and doxycycline administered in gingival sulcus of rats with induced periodontitis was achieved in a study conducted by Silveira et al (50). Also, numerous studies found that tetracycline underwent oxidation and produced reddish-colored products when exposed to light (17).…”

Section: Resultsmentioning

confidence: 59%

Comparison of Antimicrobial Effects of 445 and 970 nm Diode Laser Irradiation with Photodynamic Therapy and Triple Antibiotic Paste on Enterococcus faecalis in the Root Canal: an In Vitro Study

HOSHYARI,

MESGARANI,

SHEIKHI

et al. 2024

Maedica

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Background and aim: Colonization of residual organisms in the root canal are major causes of root canal treatment failure. Therefore, the effective removal of organisms during root canal cleaning stages is of great importance. In this study, we aimed to investigate and compare the antimicrobial effects of several methods including high power laser irradiation, photodynamic therapy and triple antibiotic paste (TAP) on Enterococcus faecalis in the root canal. Materials and methods:For the present laboratory study, 80 anterior single canal teeth were randomly divided into seven experimental groups (970 nm diode laser irradiation, 445 nm diode laser irradiation, 660 nm photodynamic therapy with doxycycline as a photosensitizer, TAP, 970 nm diode laser combined with TAP, 445 nm diode laser combined with TAP and sodium hypochlorite groups), each containing

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Section: Resultsmentioning

confidence: 59%

Comparison of Antimicrobial Effects of 445 and 970 nm Diode Laser Irradiation with Photodynamic Therapy and Triple Antibiotic Paste on Enterococcus faecalis in the Root Canal: an In Vitro Study

HOSHYARI,

MESGARANI,

SHEIKHI

et al. 2024

Maedica

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“…Doxycycline (Dox) is a synthetic bacteriostatic tetracycline effective in periodontitis 11 , 12 . As a broad-spectrum antibiotic, Dox can affect the activity of periodontal pathogens.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of nanoliposomes containing curcumin and doxycycline for enhanced antimicrobial photodynamic therapy against Aggregatibacter actinomycetemcomitans

Afrasiabi

Partoazar

Chiniforush

2023

Sci Rep

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The excessive inappropriate use of systemic antibiotics has contributed to the emergence of antibiotic-resistant pathogens, which pose a significant risk to the success of treatment. This study has approached this problem by developing doxycycline-loaded liposome doped with curcumin (NL-Cur+Dox) for combination antibacterial therapy against Aggregatibacter actinomycetemcomitans. The characterization of formulation revealed encapsulation of both drugs in NL-Cur+Dox with an average size of 239 nm and sustained release behavior. Transmission electron microscopy analysis confirmed the vesicular-shaped nanocarriers without any aggregation or crystallization. The cytotoxic and hemolytic activities of NL-Cur+Dox were evaluated. The anti-biofilm and anti-metabolic effects of NL-Cur+Dox -mediated antimicrobial photodynamic therapy (aPDT) were examined. The data indicated that NL-Cur+Dox -mediated aPDT led to a significant reduction of biofilm (82.7%, p = 0.003) and metabolic activity (75%, p < 0.001) of A. actinomycetemcomitans compared to the control. NL-Cur+Dox had no significant cytotoxicity to human gingival fibroblast cells under selected conditions (p = 0.074). In addition, the hemolytic activity of NL-Cur+Dox were negligible (< 5%). These findings demonstrate the potential application of such potent formulations in reducing one of the main bacteria causing periodontitis where the NL-Cur+Dox could be exploited to achieve an improved phototherapeutic efficiency.

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Effectiveness of Local Use of Green Propolis-Loaded Lipid Nanoparticles as Adjuvant Therapy to Scaling and Root Planing in the Management of Periodontitis in Rats Treated with Zoledronate

Silveira,

Ganzaroli,

Toro

et al. 2024

IJMS

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This study assessed the effectiveness of the local use of green-propolis-loaded lipid nanoparticles (GPlnp) as an adjuvant therapy to scaling and root planing (SRP) to manage experimental periodontitis (EP) in ovariectomized rats treated with zoledronate. Ten weeks before the experiment, 48 female rats were ovariectomized. On day 0, a ligature was installed in the lower first molar to induce EP. From day 0 to day 42, half of the rats were treated with vehicle (VEH), while the other half were treated with 100μg/Kg of zoledronate (ZOL). On day 14, the rats were allocated into the following groups: VEH-NLT, VEH-SRP, VEH-SRP-GPlnp, ZOL-NLT, ZOL-SRP, and ZOL-SRP-GPlnp. VEH-NLT and ZOL-NLT received no local treatment. VEH-SRP and ZOL-SRP received SRP and irrigation with physiological saline solution. VEH-SRP-GPlnp and ZOL-SRP-GPlnp received SRP and irrigation with GPlnp. A single SRP session was carried out, and four irrigation sessions were conducted (on days 14, 16, 18, and 20). On day 42, all animals were euthanized. The hemimandibles were processed for histological, histometric (percentage of total bone tissue (PTBT) and non-vital bone tissue (PNVBT)) and immunohistochemical (TNFα, IL-1β, and TRAP) analysis. VEH-SRP-GPlnp showed better tissue repair, higher PTBT, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with VEH. ZOL-SRP-GPlnp showed a favorable tissue repair, with lower PNVBT, less local inflammation, and lower immunolabeling for TNFα and IL-1β compared to the groups treated with ZOL. Irrigation with GPlnp proved to be effective as an adjuvant therapy to SRP in treating EP in ovariectomized rats treated with zoledronate.

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Influence of Doxycycline and InGaAlP Diode Laser at 660 nm Wavelength in the Treatment of Periodontitis Induced in Rats: In Vivo Study

Cited by 3 publications

References 43 publications

Comparison of Antimicrobial Effects of 445 and 970 nm Diode Laser Irradiation with Photodynamic Therapy and Triple Antibiotic Paste on Enterococcus faecalis in the Root Canal: an In Vitro Study

Comparison of Antimicrobial Effects of 445 and 970 nm Diode Laser Irradiation with Photodynamic Therapy and Triple Antibiotic Paste on Enterococcus faecalis in the Root Canal: an In Vitro Study

In vitro study of nanoliposomes containing curcumin and doxycycline for enhanced antimicrobial photodynamic therapy against Aggregatibacter actinomycetemcomitans

Effectiveness of Local Use of Green Propolis-Loaded Lipid Nanoparticles as Adjuvant Therapy to Scaling and Root Planing in the Management of Periodontitis in Rats Treated with Zoledronate

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