Influence of dressing changes on wound temperature

McGuiness, William; Vella, E. E.; Harrison, Debbie

doi:10.12968/jowc.2004.13.9.26702

Cited by 110 publications

(60 citation statements)

References 10 publications

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“…Furthermore, the experimental conditions chosen for this study do not ideally mimic the chronic wound environment. For instance, trauma wounds have been reported to range in temperature from 25.3 to 37.3°C28 and the wound bed temperature of chronic leg ulcers has been reported as ranging from 24°C to 26°C 29. Rather than run the experiments at 37°C, which would reflect core body temperature, our experiments were conducted at room temperature (range 19.9–23.2°C), which was closer, but not identical to, the chronic wound bed temperature.…”

Section: Discussionmentioning

confidence: 99%

Testing wound dressings using an in vitro wound model

et al. 2010

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Objective-To determine whether or not there are any significant differences in the effects of wound dressings on bacterial bioburden.Method-A selection of non-occlusive, non-adhesive dressings was tested for their effect on bacterial bioburden. The dressings selected included two dressings with antimicrobial properties (one containing silver and one containing PHMB), a cotton-based dressing enclosed in a perforated sleeve of poly(ethylene terephthalate), a carboxymethyl cellulose-based dressing, a fibre-free alginate dressing, and a 12-ply 100% cotton gauze. Using the colony-drip flow reactor (DFR) model, a meticillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa biofilm was grown underneath a dressing sample. Biofilm growth was examined via plate counts, fluorescent microscopy and scanning electron microscopy.Results-The dressings containing antimicrobial agents had the greatest effect on bacterial load. In the MRSA experiments, both antimicrobial dressings produced lower bacteria counts than the other dressings (p≤0.001), while in the P. aeruginosa experiments, only the silver-containing sample had fewer bacteria (p≤0.0001). However, neither antimicrobial dressing was able to completely eradicate the bacteria when testing with either microorganism. Conclusion-The results presented herein illustrate that bacteria can grow unchallenged within the dressing environment and that an antimicrobial dressing can limit this bacterial growth. Keywordsantimicrobial dressings; biofilms; plate counts; fluorescent microscopy; scanning electron microscopy Wounds are an ideal environment for bacterial colonisation and biofilm formation. The wound bed provides both a surface on which to grow and an ample supply of nutrients. Recent studies have shown that bacteria inoculated into wounds form biofilms. 1,2 Another study found that as many as 60% of chronic wound specimens contained a biofilm. 3 Current research indicates that biofilms are present in chronic wounds. [3][4][5][6] Furthermore, recent studies suggest that biofilms should be targeted when developing and testing therapies for these wounds. [7][8][9][10][11] A literature review of culture data from 62 published studies dating from 1969 to 1997 revealed that the predominant wound isolate in both chronic and acute wounds was Staphylococcus aureus (reported in 63% of the studies), followed by coliforms (45%), Bacteroides spp. (39%), Peptostreptococcus spp. (36%), Pseudomonas aeruginosa (29%), Enterococcus spp. (26%) and Streptococcus pyogenes (13%).

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Section: Discussionmentioning

confidence: 99%

Testing wound dressings using an in vitro wound model

et al. 2010

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“…However, it is possible to speculate that photoreceptors may not play a role in systemic infections considering the temperature and the lack of blue illumination of internal human tissues and organs targeted by this pathogen. On the other hand, the function of these receptors could be important in the pathogenesis of surface-exposed wound infections considering the potential exposure of bacteria to light and the relatively lower temperatures recorded in these types of lesions (31). It is also possible that the production of BlsA is responsible for the ability of A. baumannii to persist in medical settings, where it interacts with biotic and abiotic substrata under conditions that would require a gene expression pattern different from that expressed during the infection of the human host.…”

Section: Vol 192 2010mentioning

confidence: 99%

The Opportunistic Human PathogenAcinetobacter baumanniiSenses and Responds to Light

et al. 2010

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Light is a ubiquitous environmental signal that many organisms sense and respond to by modulating their physiological responses accordingly. While this is an expected response among phototrophic microorganisms, the ability of chemotrophic prokaryotes to sense and react to light has become a puzzling and novel issue in bacterial physiology, particularly among bacterial pathogens. In this work, we show that the opportunistic pathogen Acinetobacter baumannii senses and responds to blue light. Motility and formation of biofilms and pellicles were observed only when bacterial cells were incubated in darkness. In contrast, the killing of Candida albicans filaments was enhanced when they were cocultured with bacteria under light. These bacterial responses depend on the expression of the A. baumannii ATCC 17978 A1S_2225 gene, which codes for an 18.6-kDa protein that contains an N-terminal blue-light-sensing-using flavin (BLUF) domain and lacks a detectable output domain(s). Spectral analyses of the purified recombinant protein showed its ability to sense light by a red shift upon illumination. Therefore, the A1S_2225 gene, which is present in several members of the Acinetobacter genus, was named blue-light-sensing A (blsA). Interestingly, temperature plays a role in the ability of A. baumannii to sense and respond to light via the BlsA photoreceptor protein.

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“…However, modulation of surface-exposed wound infections by light may be of critical importance, given in addition the relatively low temperatures recorded in this type of lesion (McGuiness et al, 2004;Mussi et al, 2010). In this context, it is important to mention that some micro-organisms here reported to show modulation of antibiotic susceptibility by light, such as S. aureus and A. baumannii, are known causative agents of skin infections.…”

Section: Clinical Implications Of Light-induced Antibiotic Tolerance mentioning

confidence: 99%

White and blue light induce reduction in susceptibility to minocycline and tigecycline in Acinetobacter spp. and other bacteria of clinical importance

Ramírez

Traglia

Pérez

et al. 2015

Journal of Medical Microbiology

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Minocycline (MIN) and tigecycline (TIG) are antibiotics currently used for treatment of multidrugresistant nosocomial pathogens. In this work, we show that blue light, as well as white light, modulates susceptibility to these antibiotics in a temperature-dependent manner. The modulation of susceptibility by light depends on the content of iron; an increase in iron results in a reduction in antibiotic susceptibility both under light and in the dark, though the effect is more pronounced in the latter condition. We further provide insights into the mechanism by showing that reduction in susceptibility to MIN and TIG induced by light is likely triggered by the generation of 1 O 2 , which, by a yet unknown mechanism, would ultimately lead to the activation of resistance genes such as those coding for the efflux pump AdeABC. The clinical relevance of these results may lie in surface-exposed wound infections, given the exposure to light in addition to the relatively low temperatures recorded in this type of lesion. We further show that the modulation of antibiotic susceptibility occurs not only in Acinetobacter baumannii but also in other micro-organisms of clinical relevance such as Escherichia coli and Staphylococcus aureus. Overall, our findings allow us to suggest that MIN and TIG antibiotic treatments may be improved by the inclusion of an iron chelator, in addition to keeping the wounds in the dark, a condition that would increase the effectiveness in the control of infections involving these micro-organisms.

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Influence of dressing changes on wound temperature

Abstract: None.

Cited by 110 publications

References 10 publications

Testing wound dressings using an in vitro wound model

Testing wound dressings using an in vitro wound model

The Opportunistic Human PathogenAcinetobacter baumanniiSenses and Responds to Light

White and blue light induce reduction in susceptibility to minocycline and tigecycline in Acinetobacter spp. and other bacteria of clinical importance

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