Influence of Early Apoptosis Incidence on In Vitro Maturation of Bovine Oocytes

Aziz, Nor Azlina Abd; Osman, Nurul Atikah; Bidin, Habsah; Embong, Wan Khadijah Wan; Hashim, Noor Hashida

doi:10.1016/j.apcbee.2014.03.039

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…Numerous works have shown that the use of CC and oocyte in vitro for genotoxic and cytotoxic evaluation is a valuable and good tool for the rapid and sensitive detection of effect induced by various compound (Anguita et al, 2007;Chung et al, 2007). Furthermore, single cell gel electrophoresis (SCGE), apoptosis, and MTT assay are test used to indicate COC quality (Li et al, 2009;Osman et al, 2013;Aziz et al, 2014). Since few studies have reported the effects of eicosapentaenoic acid (EPA; an n-3 PUFA from fish oil) on bovine oocyte and CC, the aim of this study was to evaluate the influence of EPA supplementation during bovine IVM on CC expansion and oocyte nuclear maturation.…”

Section: Introductionmentioning

confidence: 99%

Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Nikoloff

Pascua

Anchordoquy

et al. 2017

Cell Biology International

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The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, and 1 mM EPA. The effect of EPA was evaluated on cumulus-oocyte complexes (COC) by oocyte maturation (cumulus expansion area and oocyte nuclear maturation), genotoxicity [single cell gel electrophoresis (SCGE)], and cytotoxicity (apoptosis, viability, and MTT assays) end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVM medium. Cumulus expansion area increased in the presence of 1 nM EPA (P < 0.05) whereas addition of 1 nM EPA (P < 0.05) decreased cumulus expansion after 24 h of IVM. Moreover, the maturation rate significantly decreased when 1 mM of EPA was assayed (P < 0.001). EPA at 1 nM induced genotoxic and cytotoxic effects on bovine cumulus cells (CC) and primary DNA lesions (P < 0.001). A significant increase in the frequency of apoptotic (P < 0.01) and necrotic (P < 0.001) cells was observed after 24 h of treatment with 1 nM, 1 μM, and 1 mM EPA. Mitochondrial activity was altered with 1 mM EPA (P < 0.001). We inferred that optimal oocyte quality was partially dependent on the presence of adequate EPA concentrations; EPA could be beneficial to improve oocyte quality in the maturation process, because low concentration tested (1 nM EPA) improved cumulus expansion.

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Section: Introductionmentioning

confidence: 99%

Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Nikoloff

Pascua

Anchordoquy

et al. 2017

Cell Biology International

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“…Estos hallazgos coinciden con otros reportes en donde se evidenció que los COC que muestran los signos de atresia temprana como una ligera expansión del cumulo y una ligera granulación del citoplasma tienen mayor o igual potencial de desarrollo que los que se consideran morfológicamente idóneos 9,17,21,28 . Llegando a la conclusión de que existe una relación positiva entre la atresia temprana y la competencia del oocito ya que se ha evidenciado una mejor tasa de maduración y de blastocistos para COC con cúmulo con expansión temprana y ooplasma granular comparado con COC con cúmulo compacto y ooplasma homogéneo 13,32 .…”

Section: Discussionunclassified

“…Aziz y colaboradores reportaron que la apoptosis temprana en CC de oocitos inmaduros bovinos está asociada a competencia oocitaria, ya que no encontraron diferencias en la capacidad de maduración in vitro entre COC con diferentes grados de apoptosis en las células del cumulo determinado por medio de tinción con connexina-V 32 . En cuanto a la capacidad de desarrollo embrionario, Li y colaboradores clasificaron los COC en cuatro grupos según características morfológicas; las tasas de clivaje y de blastocistos fueron superiores para COC con ooplasma granular, comparado con los COC con ooplasma homogéneo 21 .…”

Section: Discussionunclassified

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

Velez¹,

Chica²,

Urrego³

et al. 2017

Ces. Med. Vet. Zootec.

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The morphological selection of cumulus-oocyte complexes (COCs) is an important step for in vitro embryo production. It has been suggested that COC showing signs of atresia have the ability to generate embryos. The objective of this work was to evaluate the effect of COC morphology from Bos indicus animals with signs of early atresia versus no signs of atresia on in vitro embryo production. COC were classified in: Type I (TI): homogeneous ooplasm with ≥ 4 layers of compact cumulus cells (CC) and Type II (TII): granular ooplasm and ≥ 4 layers of CC slightly expanded. The COC were matured in vitro for 24 hours in TCM199 medium and subsequently fertilized in vitro for 18 h. The suspected zygotes were cultured in vitro for seven days in modified SOFaa medium. Embryonic quality was determined by blastomeric count following staining with Hoechst 33342. Student test was used to determine statistical differences for cleavage, blastocyst rate and blastomeric counts between types of COC. The cleavage rate for TI (n = 220) and TII (n = 161) was 88 ± 4 % and 89 ± 8 % respectively (p> 0.05); embryo development rate was 36 ± 7 % and 33 ± 8 % (p>0.05) respectively. The blastomeric count for both groups was 101 and 104 cells for TI and TII respectively (n = 10), (p>0.05). These results demonstrate that there is no difference in the quantity and quality of embryos produced in vitro using COC type I or type II, suggesting that both types could be used for bovine in vitro embryo production in Bos indicus cows. Keywords: atresia, competence, cumulus-oocyte complex.Resumen La selección morfológica de los complejos cúmulo-oocito (COC) es crucial para la producción de embriones in vitro. Se ha sugerido que COC que muestran signos de atresia poseen capacidad de generar embriones. El objetivo de este trabajo fue evaluar el efecto de la morfología de los COC provenientes de animales Bos indicus con signos de atresia temprana y sin signos de atresia sobre la producción de embriones in vitro. Se clasificaron COC obtenidos de ovarios de faenado en dos grupos: Tipo I (TI): ooplasma homogéneo con ≥ 4 capas de células del cumulo (CC) compactas y Tipo II (TII): ooplasma

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“…Oocytes matured in vivo degenerate if not fertilized. In the ovary, oocytes showing early signs of atresia have high developmental potential (Aziz et al, 2014). Therefore, most oocytes collected from slaughtered animals by slicing will have arisen from atretic follicles.…”

Section: Discussionmentioning

confidence: 99%

“…For gonadotropins to act in vitro, FSHR and LHR genes must be expressed by cumulus cells (Xiao et al, 2014). Gonadotropin-releasing hormone receptors (GnRHRs) are expressed in ovaries (Wei et al, 2013), but their expression in sheep cumulus-oocyte complexes (COCs) has not been described in detail (Aziz et al, 2014;Karami-Shabankareh et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression

et al. 2016

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We assessed the effects of equine chorionic gonadotropin (eCG) on oocyte in vitro maturation (IVM), apoptosis, and follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), and gonadotropin-releasing hormone receptor (GnRHR) expression and mRNA levels. Cumulus-oocyte complexes (COCs) were recovered from sheep ovaries and pooled in groups, before being cultured in IVM media containing varying eCG concentrations. Maturation and apoptosis rates were then calculated. Expression of FSHR, LHR, and GnRHR mRNA in oocytes was measured using quantitative reverse transcription polymerase chain reaction. Protein levels were ascertained by western blotting. Matured oocytes displayed and released an intact first polar body. Sheep oocyte maturation rates gradually increased as eCG concentration was raised from 0 to 20 µg/mL. Apoptosis rates of eCG-treated oocytes were lower than those of the control group, and were lowest using 20 µg/mL eCG. FSHR, LHR, and GnRHR mRNA expression increased (P < 0.01, P < 0.05, and P < 0.05, respectively, compared to 0 µg/mL eCG) with eCG concentration, being highest following exposure to 20 µg/mL. FSHR and GnRHR protein levels were significantly higher in oocytes administered 20 µg/mL eCG compared with those matured in the absence of eCG. eCG dose positively correlated with FSHR, LHR, and GnRHR mRNA and protein expression. In conclusion, eCG enhances maturation and decreases apoptosis of oocytes undergoing IVM, and heightens FSHR, LHR, and GnRHR expression. Such increased expression may facilitate oocyte IVM. These findings contribute to our understanding of the mechanisms of underlying hormonal control of sheep oocyte IVM, advancing ovine reproductive methods.

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Influence of Early Apoptosis Incidence on In Vitro Maturation of Bovine Oocytes

Cited by 6 publications

References 19 publications

Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Producción in vitro de embriones a partir de complejos cúmulos oocitos tipo II en bovinos Bos indicus

Equine chorionic gonadotropin influence on sheep oocyte in vitro maturation, apoptosis, and follicle-stimulating hormone receptor and luteinizing hormone receptor expression

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