Influence of eggshell ultrastructural organization on hatchability

Liao, Bing; Qiao, Hai-Li; Zhao, Xu; Bao, Man; Liu, L.; Zheng, Chuan; Li, C. F.; Ning, Zhonghua

doi:10.3382/ps.2012-02728

Cited by 30 publications

(23 citation statements)

References 18 publications

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“…The distribution of mammillae and organic constituents of the scaffold affect structural aspects, such as mammillary cone size, palisade morphology, as well as mechanics of the bioceramic [46,47]. To gain a better insight, morphological features of the shell layers are revisited by applying tools of microscopy.…”

Section: Architecture Of the Organic-inorganic Interfacementioning

confidence: 99%

“…This suggests an interlocking of the scaffold with the palisade cones at mammillae sites, serving as a robust interface between the organic and inorganic layers of the mature biomineral. mammillary cone size, palisade morphology, as well as mechanics of the bioceramic [46,47]. To gain a better insight, morphological features of the shell layers are revisited by applying tools of microscopy.…”

Section: Architecture Of the Organic-inorganic Interfacementioning

confidence: 99%

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On Mineral Retrosynthesis of a Complex Biogenic Scaffold

2017

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Synergistic relations between organic molecules and mineral precursors regulate biogenic mineralization. Given the remarkable material properties of the egg shell as a biogenic ceramic, it serves as an important model to elucidate biomineral growth. With established roles of complex anionic biopolymers and a heterogeneous organic scaffold in egg shell mineralization, the present study explores the regulation over mineralization attained by applying synthetic polymeric counterparts (polyethylene glycol, poly(acrylic acid), poly(aspartic acid) and poly(4-styrenesulfonic acid-co-maleic acid)) as additives during remineralization of decalcified eggshell membranes. By applying Mg 2+ ions as a co-additive species, mineral retrosynthesis is achieved in a manner that modulates the polymorph and structure of mineral products. Notable features of the mineralization process include distinct local wettability of the biogenic organic scaffold by mineral precursors and mineralization-induced membrane actuation. Overall, the form, structure and polymorph of the mineralization products are synergistically affected by the additive and the content of Mg 2+ ions. We also revisit the physicochemical nature of the biomineral scaffold and demonstrate the distinct spatial distribution of anionic biomolecules associated with the scaffold-mineral interface, as well as highlight the hydrogel-like properties of mammillae-associated macromolecules.

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Section: Architecture Of the Organic-inorganic Interfacementioning

confidence: 99%

Section: Architecture Of the Organic-inorganic Interfacementioning

confidence: 99%

On Mineral Retrosynthesis of a Complex Biogenic Scaffold

2017

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“…The shell membranes were then carefully peeled from the edge of the sample inward (this step was missed out for cross section analysis). The egg shell samples were then boiled in 2% NaOH for 10 min to dissolve any proteinaceous material incorporated in the shell (Liao et al, 2013). After drying thoroughly at room temperature, the shell samples were then mounted on aluminium stubs using conductive silver paint (1005 aqueous conductive silver liquid-SEM adhesive, ProSciTech, Kirwan, Australia), sputter coated with gold in a Jeol MP-19020NCTR Neocoater and viewed with a XL-30 environmental scanning electron microscope (Philips, Amsterdam, The Netherlands).…”

Section: Microstructural Analysis Of Egg Shellmentioning

confidence: 99%

Dietary calcium deficiency in laying ducks impairs eggshell quality by suppressing the process of shell biomineralization

Chen

Zhao

Tian

et al. 2015

Journal of Experimental Biology

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The objective of this study was to determine the effects of dietary calcium deficiency on the process of shell formation. Four hundred and fifty female ducks (Anas platyrhynchos) at 22 weeks were randomly assigned to three groups. Ducks were fed one of two calcium-deficient diets (containing 1.8% or 0.38% calcium, respectively) or a calcium-adequate control diet (containing 3.6% calcium) for 67 days (depletion period) and then all ducks were fed a calcium-adequate diet for an additional 67 days (repletion period). Compared with the calcium-adequate control, the average shell thickness, egg shell weight, breaking strength, mammillae density and mammillary knob thickness of shell from ducks that consumed the diet with 0.38% calcium were significantly decreased (P<0.05) during the depletion period, accompanied by reduced tibia quality. The mRNA expression of both secreted phosphoprotein 1 (SPP1) and carbonic anhydrase 2 (CA2) in the uterus was decreased after feeding calcium-deficient diets (1.8% or 0.38% calcium). mRNA transcripts of calbindin 1 (CALB1), an important protein responsible for calcium transport, and the matrix protein genes ovocalyxin-32 (OCX-32) and ovocleidin-116 (OC-116) were reduced in ducks fed 0.38% calcium but not 1.8% calcium. Plasma estradiol concentration was decreased by both of the calciumdeficient diets (P<0.05). The impaired shell quality and suppressed functional proteins involved in shell formation could be reversed by repletion of dietary calcium. The results of the present study suggest that dietary calcium deficiency negatively affects eggshell quality and microarchitecture, probably by suppressing shell biomineralization.

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“…Previous studies have shown that eggshell ultrastructure organization, including eggshell thickness and mammillary layer thickness, influences both eggshell quality and egg hatchability. In addition, mammillary cone size contributes to the mechanical properties of the eggshell (Liao et al, 2013). Thus, appropriate eggshell ultrastructure organization is critical for eggshell quality in hens.…”

Section: Introductionmentioning

confidence: 99%

Gene Polymorphisms are Associated with Eggshell Ultrastructure Organization in Hens

Wang

Liao²,

Zhang

et al. 2017

Rev. Bras. Cienc. Avic.

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Background: Eggshell ultrastructure organization, including effective layer thickness, mammillary layer thickness, and average size of mammillary cones, is important for breeding and significantly influences eggshell mechanical properties. Several matrix proteins were known to be important in eggshell formation. However, the proteins and variations that determine eggshell ultrastructure organization are not known.Results: In this study, 17 single-nucleotide polymorphisms of three major genes in a hen population using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Five single-nucleotide polymorphisms with a very low minor allele frequency (< 1%) were excluded from further analysis. The remaining 12 single-nucleotide polymorphisms in Hardy-Weinberg equilibrium were used for analysis of associations with eggshell ultrastructure organization. Associations were found for (i) ovocleidin-116 with effective layer thickness (EFF), mammillary layer thickness (MAM), and average size of mammillary cones (SMAM); (ii) ovalbumin with eggshell thickness (ESH), effective layer thickness, and density of the mammillary cone (DMAM); and (iii) calmodulin1 with density of the mammillary cone.Conclusions: The single-nucleotide polymorphisms identified in the present study may be used as potential markers to improve eggshell quality.

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Influence of eggshell ultrastructural organization on hatchability

Cited by 30 publications

References 18 publications

On Mineral Retrosynthesis of a Complex Biogenic Scaffold

On Mineral Retrosynthesis of a Complex Biogenic Scaffold

Dietary calcium deficiency in laying ducks impairs eggshell quality by suppressing the process of shell biomineralization

Gene Polymorphisms are Associated with Eggshell Ultrastructure Organization in Hens

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