Influence of electro-ejaculator on serum concentrations of acute phase proteins and bone metabolism biomarkers in male dromedary camels (<i>Camelus dromedarius</i>)

Tadjine, Mohamed; Al-Sobayil, Fahd

doi:10.1080/09712119.2018.1490299

Cited by 8 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EJ in dromedary is very stressful, causes a reversible myocardial injury and increases the serum concentrations of lactic acid leading to a disturbance in acid-base balance (Tharwat et al, 2014). Also, use of EJ in camels increases some indicators of inflammatory disturbances such as acute phase proteins and bone metabolism biomarkers (Tharwat & Al-Sobayil, 2018). Therefore, in our trials with EJ only three runs of programmed stimulation cycles were applied, regardless a complete ejaculation occurred or not, to avoid the harmful effect of the prolonged electrical stimulation.…”

Section: Discussionmentioning

confidence: 99%

A novel, patented method for semen collection in dromedary camel (Camelus dromedarius)

Mansour

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

In the current article, a developed, patented method denoted the ‘Camel Semen Collection Kit‐CSCK’, was designed to solve the problem of semen collection in dromedary camels. CSCK is composed of three main parts: (1) Semen collection sac: made from supersensitive flexible low‐density polyethylene‐ (LDPE); (2) Metal stainless steel applicator: designed to introduce the collection sac intravaginally and fixate it to the vaginal wall of a female camel through air insufflation; (3) Fixation sticker: a cushion sheet sticker is used to secure the outer portion of the collection sac to the female's perineal area. Semen was collected twice a week from eight dromedary bulls by using electroejaculation (EJ), artificial vagina (AV) and CSCK. Successful semen collections were 81.3%, 84.4% and 43.8% using EJ, CSCK and AV techniques respectively. Semen obtained by EJ technique showed lower semen volume, gross activity, sperm concentration, total sperm motility and percentage of live sperm cells compared to the other two techniques. Semen collected by CSCK showed a longer collection period and higher volume, gross activity, sperm motility and percentage of live spermatozoa and a lower rate of visible contamination compared to AV technique. The advantages and disadvantages of the three techniques were compared and discussed. In conclusion, CSCK represents a practical and easy method to reliably collect high‐quality semen from any untrained male dromedary camel and may facilitate the widespread application of assisted reproductive technologies (ARTs) on a large scale in this species.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel, patented method for semen collection in dromedary camel (Camelus dromedarius)

Mansour

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…A monoclonal antibody specific for SAA has been coated onto the wells of the microliter strips provided. Concentration of the second inflammation biomarker haptoglobin (Hp) in sera was determined using a colorimetric assay (Haptoglobin kit, second generation, Tridelta Ltd., Ireland), as described previously Tharwat and Al-Sobayil 2015a,b;Tharwat and Al-Sobayil 2018a;Tharwat 2020a).…”

Section: Inflammatory Biomarkers Assaymentioning

confidence: 99%

“…Infection or inflammation biomarkers or acute phase proteins (APPs) are serum proteins, and blood concentrations of these proteins can vary after infection, inflammation, or trauma (Murata et al 2004;Tharwat and Al-Sobayil 2015a,b;Tharwat and Al-Sobayil 2018a;Tharwat 2020a). This phenomenon is usually called the acute phase response or reaction (APR) that mostly occurs secondary to various stimuli of tissue damage; however, it may also be physiological during inflammatory episodes (Yazwinsk et al 2013).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Acute Blood Loss on Inflammatory and Bone Biomarkers, Acid-base Balance, Blood Gases and Hemato-biochemical Profiles in Sedated Donkeys (Equus asinus)

2022

Int J Vet Sci

View full text Add to dashboard Cite

The current experiment was designed to investigate the possible effects of acute blood loss on the inflammatory and bone biomarkers status, acid-base balance, blood gases, and hemato-biochemical profiles in sedated donkeys (Equus asinus). For the induction of acute blood loss, collected 900mL of blood from the carotid artery of 10 male donkeys sedated with xylazine HCl (1mg/kg). For analysis, five blood samples were collected; collected the 1st (T0) before induction of blood loss and the other 4 (T1, T2, T3 and T4) collected at 30, 60, 120 and 240min post-blood loss. The serum concentrations of amyloid A (SAA) and haptoglobin (Hp) decreased at all-time points after blood loss compared to T0, but the differences were non-significant. The differences in levels of serum biomarkers osteocalcin (OC), β-alkaline phosphatase (β-ALP) and pyridinoline cross-links (PYD) at all-time points post blood loss compared to T0 values were also non-significant. Except for PCO2, the acid-base status and blood gases, including pH, PO2, HCO3, TCO2, sO2, base excess, anion gap and lactate, increased significantly after blood withdrawal. On the contrary, most of the measured hemato-biochemical parameters decreased significantly after blood loss, except for glucose. In conclusion, acute blood loss in sedated donkeys did not influence serum concentrations of the inflammatory biomarkers SAA and Hp and the markers of bone metabolism OC, β-ALP, and PYD. On the contrary, most acid-base, blood gas parameters, and hemato-biochemical parameters differed significantly after blood withdrawal compared to their values before blood loss.

show abstract

“…e bone biomarkers OC, b-ALP, and PYD serum concentrations were determined in the serum using commercial immunoassay kits (Quidel Corp., CA, USA) as our group described recently [17][18][19][20]. e limit of quantification of OC ranged from 2 to 32 ng/mL, and precision CVs within runs and between runs were 5-10%.…”

Section: Bone Metabolism Biomarker Assaysmentioning

confidence: 99%

Serum Concentration of Bone Metabolism Biomarkers in Goats during the Transition Period

Tadjine

2020

Veterinary Medicine International

Self Cite

View full text Add to dashboard Cite

Objective. During the transition period, the animal experiences a series of nutritional, physiological, and social changes. e objective of the present study was to evaluate the influence of the periparturient period in goats on the serum concentrations of the bone biomarkers osteocalcin (OC), bone-specific alkaline phosphatase (b-ALP), and pyridinoline cross-links (PYD). Method. Blood samples were collected from fifteen female goats during the periparturient period 3 wk before expected parturition (T − 3), within 12 h of parturition (T 0), and 3 wk after parturition (T +3). Results. Compared to a value of 77.67 ± 47.6 ng/mL at T − 3, the serum concentrations of OC measured 51.91 ± 22.09 ng/mL at T 0 and 72.61 ± 35.21 ng/mL at T +3. A comparison of OC values at T − 3, T 0, and T +3 did not reveal any significant difference (P > 0.05). Compared to a value of 42.00 ± 19.50 U/L at T − 3, the serum concentration of b-ALP measured 32.49 ± 15.41 U/L at T 0 and 34.31 ± 18.89 U/L at T +3. A comparison of b-ALP values at T − 3, T 0, and T +3 did not reveal any significant difference (P > 0.05). Compared to a value of 17.86 ± 9.15 nmol/L at T − 3, the serum concentration of PYD decreased significantly at T 0 to a value of 7.48 ± 4.50 nmol/L (P < 0.0001). At T +3, the PYD serum concentration measured 7.72 ± 2.91 nmol/L, which differed significantly from T − 3 values (P < 0.05). Conclusion. e results of this study showed that the bone formation biomarkers (OC and b-ALP) did not change significantly during the periparturient period, while the bone resorption biomarker decreased significantly at parturition compared to 3 wk before and 3 wk after parturition. e significantly increased serum estrogen around parturition may have had a role in the increased bone resorption at this time.

show abstract

Influence of electro-ejaculator on serum concentrations of acute phase proteins and bone metabolism biomarkers in male dromedary camels (Camelus dromedarius)

Cited by 8 publications

References 44 publications

A novel, patented method for semen collection in dromedary camel (Camelus dromedarius)

A novel, patented method for semen collection in dromedary camel (Camelus dromedarius)

The Effects of Acute Blood Loss on Inflammatory and Bone Biomarkers, Acid-base Balance, Blood Gases and Hemato-biochemical Profiles in Sedated Donkeys (Equus asinus)

Serum Concentration of Bone Metabolism Biomarkers in Goats during the Transition Period

Contact Info

Product

Resources

About