Influence of electrode surface charge on current production by Geobacter sulfurreducens microbial anodes

Frühauf, Hanna Marianne; Holtmann, Dirk; Stöckl, Markus

doi:10.1016/j.bioelechem.2022.108213

Cited by 7 publications

(3 citation statements)

References 55 publications

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“…The ATR-FTIR spectra of G. sulfurreducens biofilms cultivated on Fe(III) hydroxide revealed details in the molecular interactions between bacterial cells and the Fe(III) hydroxide surface. It should be noted that, generally, at pH around 7 bacteria are negatively charged, including G. sulfurreducens [ 65 ]. Iron oxide surface has a positive charge in this pH range and therefore, bacterial adhesion is electrostatically favored [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate

Yunda

Björn

et al. 2023

Biofilm

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Section: Discussionmentioning

confidence: 99%

Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate

Yunda

Björn

et al. 2023

Biofilm

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“…All methods are described in detail in (Frühauf et al, 2022; Frühauf et al, 2022) and are only briefly described here. G. sulfurreducens strain PCA (DSM 12127) was obtained from DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).…”

Section: Methodsmentioning

confidence: 99%

Geobacter sulfurreducens metabolism at different donor/acceptor ratios

Frühauf-Wyllie

Holtmann

2022

MicrobiologyOpen

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Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target-oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of Geobacter sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized.central metabolism, donor/acceptor ratio, Geobacter sulfurreducens, organic acid production | INTRODUCTIONGeobacter strains are deltaproteobacteria and can be found in different habitats, for example, aquatic systems, deep aquifer sediments, subsurface sediments, as well as several contaminated sites. Geobacter cells are Gram-negative, nonspore-forming, and obligate heterotrophs (Straub, 2011;Zhang et al., 2020). In general, Geobacter species grow only under strictly anoxic conditions and are unable to grow by fermentation (Straub, 2011). Geobacter species predominate under iron-reducing conditions (Straub, 2011). Acetate is a common electron and carbon donor among Geobacter species (Straub, 2011) and the majority of species could also metabolize alternative electron donors (e.g., ethanol, pyruvate, lactate, hydrogen, and formate). Geobacter species are using different electron acceptors, for example, Fe(III), nitrate, elemental sulfur (S 0 ), fumarate, malate, Mn(IV), U(VI), Co(III), and humic substances.Geobacter species appear to be the primary agents for coupling the oxidation of organic compounds to the reduction of insoluble Fe(III) and Mn(IV) oxides in many soils and sediments (Lovley et al., 2011).Different Geobacter species can oxidize aromatic hydrocarbons under anaerobic conditions. In addition, Geobacter species are important for aromatic hydrocarbon removal in contaminated

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“…145 Electrostatic self-assembly of charged polymers was used to systematically modify the surface of indium-tin-coated electrodes, wherein the thickest biofilms, highest current density and shortest start-up time were achieved for negatively charged electrode surfaces or polystyrol sulfonate-coated electrodes, while positively charged chitosan, negatively charged alginate and positively charged polyethylene imine produced thinner biofilms with less current and a longer start-up time. 146 Surface functional groups also affect the attachment of microorganisms, the formation of biofilm and electron transfer. C-, N-, O- and S-containing functional groups were reported to enhance microbial attachment at the interface.…”

Section: The Strategies For Constructing Efficient Material–microorga...mentioning

confidence: 99%

The material–microorganism interface in microbial hybrid electrocatalysis systems

et al. 2023

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Influence of electrode surface charge on current production by Geobacter sulfurreducens microbial anodes

Cited by 7 publications

References 55 publications

Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate

Biochemical characterization and mercury methylation capacity of Geobacter sulfurreducens biofilms grown in media containing iron hydroxide or fumarate

Geobacter sulfurreducens metabolism at different donor/acceptor ratios

The material–microorganism interface in microbial hybrid electrocatalysis systems

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