Influence of endogenous and exogenous RNases on the variation of pollen cytosolic-free Ca2+ in Pyrus serotina Rehd

Xu, Guohua; Zhang, Shaoling; Yang, Yu-Hang; Zhao, Chao; Wolukau, Joseph N.

doi:10.1007/s11738-007-0112-8

Cited by 9 publications

(3 citation statements)

References 33 publications

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“…We detected three ABC transporters in which the expression profile of the ABCA gene was similar to MdABCF [ 54 ] and significantly associated with S-RNase transport. Calcium is the second messenger of cell signal transduction, and previous studies have confirmed that the Ca 2+ gradient is involved in the self-incompatibility of pears [ 31 , 40 ]. We have detected three genes related to the formation of calcium ion gradients and its signal transduction.…”

Section: Discussionmentioning

confidence: 99%

“…This involves a complex genetic mechanism, and the display of the complete trait of self-incompatibility not only depends on the S-site genes (pistil S gene and pollen S gene) but, also, several modifier genes associated with complex mechanisms that have been gradually explored [ 29 ]. These modifier genes are involved in multiple signal transduction systems [ 29 ], for example, calcium ion signaling [ 29 , 30 , 31 ], reactive oxygen signaling [ 32 ], hormone signaling [ 33 ], inositol phosphate signaling [ 34 , 35 ], biochemical metabolism processes [ 36 ], programmed cell death (PCD) [ 32 , 37 ], and cell wall and cytoskeletal construction [ 28 , 38 , 39 , 40 , 41 ]. These modifiers, distributed in a determined location of the chromosome, will affect the intensity of self-incompatibility to varying degrees, and be directly reflected in the fruit-set rate.…”

Section: Introductionmentioning

confidence: 99%

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Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by Resequencing Fragaria viridis

Xiong

et al. 2019

IJMS

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It is clear that the incompatibility system in Fragaria is gametophytic, however, the genetic mechanism behind this remains elusive. Eleven second-generation lines of Fragaria viridis with different compatibility were obtained by manual self-pollination, which can be displayed directly by the level of fruit-set rate. We sequenced two second-generation selfing lines with large differences in fruit-set rate: Ls-S2-53 as a self-incompatible sequencing sample, and Ls-S2-76 as a strong self-compatible sequencing sample. Fragaria vesca was used as a completely self-compatible reference sample, and the genome-wide variations were identified and subsequently annotated. The distribution of polymorphisms is similar on each chromosome between the two sequencing samples, however, the distribution regions and the number of homozygous variations are inconsistent. Expression pattern analysis showed that six candidate genes were significantly associated with self-incompatibility. Using F. vesca as a reference, we focused our attention on the gene FIP2-like (FH protein interacting protein), associated with actin cytoskeleton formation, as the resulting proteins in Ls-S2-53 and Ls-S2-76 have each lost a number of different amino acids. Suppression of FIP2-like to some extent inhibits germination of pollen grains and growth of pollen tubes by reducing F-actin of the pollen tube tips. Our results suggest that the differential distribution of homozygous variations affects F. viridis fruit-set rate and that the fully encoded FIP2-like can function normally to promote F-actin formation, while the new FIP2-like proteins with shortened amino acid sequences have influenced the (in)compatibility of two selfing lines of F. viridis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by Resequencing Fragaria viridis

Xiong

et al. 2019

IJMS

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“…We have developed a protocol for the successful isolation of spheroplasts from the pollen tube of the pear, identified a hyperpolarization‐activated cation channel using the patch‐clamp technique (Qu et al. , 2007) and discovered the relationship between the Ca 2+ ‐permeable channels of the pollen tube and self‐S‐RNase activity (Xu et al. , 2007).…”

Section: Introductionmentioning

confidence: 99%

S‐RNase triggers mitochondrial alteration and DNA degradation in the incompatible pollen tube of Pyrus pyrifolia in vitro

Wang

Jiang

et al. 2009

The Plant Journal

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SummaryPear (Pyrus pyrifolia L.) has a S-RNase-based gametophytic self-incompatibility (SI) mechanism, and S-RNase has also been implicated in the rejection of self-pollen and genetically identical pollen. No studies, however, have examined the extent of organelle alterations during the SI response in Pyrus pyrifolia. Consequently, this study focused on the alterations to mitochondria and nuclear DNA in incompatible pollen tubes of the pear. Methylthiazolyldiphenyl-tetrazolium bromide was used to evaluate the viability of pollen tubes under S-RNase challenge. The results showed that the viability of the control and compatible pollen tubes decreased slightly, but that of the incompatible pollen and pollen tubes began to decline at 30 min. The mitochondrial membrane potential (Dw mit ) was also tested with rhodamine 123 30 min after SI challenge, and was shown to have collapsed in the incompatible pollen tubes after exposure to S-RNase. Western blotting 2 h after SI challenge confirmed that the Dw mit collapse induced leakage of cytochrome c into the cytosol. Swollen mitochondria were detected by transmission electron microscopy as early as 1 h after SI challenge and the degradation of nuclear DNA was observed by both 4,6-diamidino-2-phenylindole and transferase-mediated dUTP nick-end labeling. These diagnostic features of programmed cell death (PCD) suggested that PCD may specifically occur in incompatible pollen tubes.

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The activity of plasma membrane hyperpolarization-activated Ca2+ channels during pollen development of Pyrus pyrifolia

Jin

et al. 2011

Acta Physiol Plant

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Influence of endogenous and exogenous RNases on the variation of pollen cytosolic-free Ca2+ in Pyrus serotina Rehd

Cited by 9 publications

References 33 publications

Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by Resequencing Fragaria viridis

Identifying Genome-Wide Sequence Variations and Candidate Genes Implicated in Self-Incompatibility by Resequencing Fragaria viridis

S‐RNase triggers mitochondrial alteration and DNA degradation in the incompatible pollen tube of Pyrus pyrifolia in vitro

The activity of plasma membrane hyperpolarization-activated Ca2+ channels during pollen development of Pyrus pyrifolia

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