Influence of energy and wavelength on femtosecond laser-induced nucleation of protein

Murai, Ryosuke; Yoshikawa, Hiroshi; Hasenaka, Hitoshi; Takahashi, Yoshinori; Maruyama, Mihoko; Sugiyama, Shigeru; Adachi, Hiroaki; Takano, Kazufumi; Matsumura, Hideki; Murakami, Satoshi; Inoue, Tsuyoshi; Mori, Yusuke

doi:10.1016/j.cplett.2011.05.016

Cited by 16 publications

(19 citation statements)

References 27 publications

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“…According to Nakamura et al 53 the femto-second laser-induced shockwave and cavitation bubble generation processes are key factors for crystallization. Later, similar experimental works [54][55][56][57][58] confirmed the provided explanation for the nucleation mechanism in these experimental conditions.…”

Section: Proteins Moleculessupporting

confidence: 70%

Experimental Demonstration of the Carbamazepine Crystallization from Non-photochemical Laser-Induced Nucleation in Acetonitrile and Methanol

Ikni

Clair

Scouflaire

et al. 2014

Crystal Growth & Design

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International audienceThis paper reports for the first time the crystallization of the carbamazepine (CBZ) molecule in two solvents (methanol and acetonitrile) using the non-photochemical laser-induced nucleation (NPLIN) technique. The metastable zone of CBZ is first determined experimentally for different temperatures in both solvents. Then, the prepared solutions are irradiated by a 532 nm wavelength nanosecond pulsed laser and permitted to obtain CBZ crystals of phases I and III. The impact of laser power and polarization (circularly (CP) and linearly (LP)) on the CBZ crystallization efficiency in both solvents is determined through experiments. According to the results, the crystallization efficiency is significantly higher in methanol than in acetonitrile, and it is higher in solutions irradiated by CP laser than those by LP laser. Moreover, the irradiation of an acetonitrile solution by a LP laser results in CBZ phases I and III, whereas irradiation by the CP laser leads to CBZ phase III crystals. An ab initio determination of the interaction energy of different pairs of CBZ has been carried-out that enables the explanation of the nucleation in acetonitrile for both polarizations. In methanol, only CBZ phase III is obtained, which is in agreement with the ability of methanol to create noncovalent interactions preventing the CBZ phase I and II nucleation

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Section: Proteins Moleculessupporting

confidence: 70%

Experimental Demonstration of the Carbamazepine Crystallization from Non-photochemical Laser-Induced Nucleation in Acetonitrile and Methanol

Ikni

Clair

Scouflaire

et al. 2014

Crystal Growth & Design

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show abstract

“…The perturbation arising from these phenomena in the surrounding media near the focal point of the laser beam can trigger the nucleation. The present experimental observations are consistent with earlier studies in similar direction (Yoshikawa et al, 2006;Murai et al, 2011).…”

Section: Possible Mechanism Of Nucleation Under Laser Irradiationsupporting

confidence: 83%

Small and Macromolecules Crystallization Induced by Focused Ultrafast Laser

Shilpa¹,

Bhat²,

Rodrigues³

et al. 2015

Proceedings of the Indian National Science Academy

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The structure based drug design has been limited by various factors that include protein crystallization, which is one of the most challenging tasks in this area. It has been proved unequivocally that X-ray crystallography is highly trusted technique for three dimensional structure determination of small-and macro-molecules. It has provided the definite solution for several key areas such as structure based drug design, site directed mutagenesis and elucidation of enzyme mechanisms. In X-ray crystallography, growing good quality of crystals for structure determination is always, the rate limiting step. Various approaches including laser induced crystallization technique has been reported to tackle this issue. We report on the crystallization of small-and macro-molecules under focused optical radiation from a femtosecond laser (λ = 800 nm). The efficacy of this technique is proved by crystallizing different samples such as sodium chloride and urea, and by comparing the results to those crystals obtained by conventional methods. In addition, the unique capability of the developed technique is demonstrated by crystallizing the three chalcone compounds that are difficult to crystallize using conventional methods. Furthermore, the developed technique is extended to crystallize protein molecule (lysozyme) and the results indicate that present technique is a potential alternative tool to crystallize biomolecules.

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“…However, Murai et al reported that the dimer contribution to nucleation is rather small in the femtosecond laser case, because the enhancement of nucleation by laser irradiation at l = 260 nm (producing more dimers) is the same as that at l = 780 nm. 39 One of the reasons why the contribution is small in the femtosecond laser case may be because femtosecond laser ablation would cause rather protein denaturation than dimer formation in the absorption volume. Murai et al found that focused femtosecond laser irradiation produced not only dimers but also white, visibly large aggregates of denatured HEWL, which did not act as seeds for nucleation.…”

Section: Nucleation Mechanism Based On Femtosecond Laser Ablationmentioning

confidence: 99%

“…Murai et al found that focused femtosecond laser irradiation produced not only dimers but also white, visibly large aggregates of denatured HEWL, which did not act as seeds for nucleation. 39 Since a femtosecond laser has a higher peak intensity [BGW] than a Xe lamp [B100 W], proteins rather undergo the simultaneous and/or stepwise multiphoton absorption, which can produce the permanently damaged species. 37 Fig.…”

Section: Nucleation Mechanism Based On Femtosecond Laser Ablationmentioning

confidence: 99%

Laser ablation for protein crystal nucleation and seeding

et al. 2014

Self Cite

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With the recent development in pulsed lasers with ultrashort pulse widths or wavelengths, spatially precise, low-damage processing by femtosecond or deep-UV laser ablation has shown promise for the production of protein single crystals suitable for X-ray crystallography. Femtosecond laser processing of supersaturated solutions can shorten the protein nucleation period or can induce nucleation at low supersaturation, which improves the crystal quality of various proteins including membrane proteins and supra-complexes. In addition to nucleation, processing of protein crystals by femtosecond or deep-UV laser ablation can produce single crystalline micro- or macro-seeds without deterioration of crystal quality. This tutorial review gives an overview of the successful application of laser ablation techniques to nucleation and seeding for the production of protein single crystals, and also describes the advantages from a physico-chemical perspective.

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Influence of energy and wavelength on femtosecond laser-induced nucleation of protein

Cited by 16 publications

References 27 publications

Experimental Demonstration of the Carbamazepine Crystallization from Non-photochemical Laser-Induced Nucleation in Acetonitrile and Methanol

Experimental Demonstration of the Carbamazepine Crystallization from Non-photochemical Laser-Induced Nucleation in Acetonitrile and Methanol

Small and Macromolecules Crystallization Induced by Focused Ultrafast Laser

Laser ablation for protein crystal nucleation and seeding

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