Influence of exogenous polyamines and plant growth regulators on high frequency in vitro mass propagation of Gloriosa superba L. and its colchicine content

Shanmugam, Sivakumar; Siva, G.; Sathish, Selvam; Kumar, G. Prem; Vigneswaran, M.; Vinoth, S.; Kumar, T. Senthil; Ramalingam, Sathishkumar; Jayabalan, N.

doi:10.1016/j.bcab.2019.101030

Cited by 17 publications

(4 citation statements)

References 50 publications

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“…Combining higher concentration of 6-BA and lower concentration of NAA favors bud elongation. This is in agreement with the results of Sivakumar S et al They reported that the bud growth was best in basic medium supplemented with 6-BA 1.5 mg/L and NAA 0.6 mg/L (Sivakumar et al, 2019). However adventitious bud grew slower and weak in the later stage.…”

Section: Shoot Inductionsupporting

confidence: 92%

Micropropagation of Ilex cassine ‘angustifolia’

Gui

Zou

Zhang

et al. 2023

Preprint

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For mass propagation of Ilex cassine ‘angustifolia’, tissue culture was used to shorten its propagation cycle. We sterilized its nodal segments as explants and then cultured them on MS basic medium supplemented with different concentrations of 6-BA and NAA, and placed them in the culture room for the induction and proliferation of adventitious shoots. The highest induction rate of MS medium with 6-BA 1.0 mg/L and NAA 1.0 mg/L was used for the induction of adventitious buds in Ilex cassine ‘angustifolia’ nodal segments, reaching 80%. Buds sprouted on the 10th day, while the average value of bud length reached 1.62 cm at 30 days. The adventitious buds grew fast and robust. The best proliferation coefficient was 5.7 when explants were cultured on MS basic medium containing 6-BA mg/Land NAA 0.5 mg/L. For adventitious root induction, the adventitious shoots were transferred to MS basic medium with different concentrations of IBA and NAA. The results showed that the highest rooting rate of 98.7% was achieved when explants were cultured on MS basic medium supplemented with NAA 0.5 mg/L and IBA 1.0 mg/L, and the adventitious roots induced were thick and robust. Rooted sterile seedlings had been planted in the substrate and transferred to a greenhouse with sprinkler irrigation for further cultivation. The survival rate was about 90%.

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Section: Shoot Inductionsupporting

confidence: 92%

Micropropagation of Ilex cassine ‘angustifolia’

Gui

Zou

Zhang

et al. 2023

Preprint

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“…Most shoots are obtained in the presence of 9.84 μM 2iP combined with 4.64 μM Kin after 21-day culturing. Sivakumar et al (2019) developed an efficient protocol for in vitro mass propagation of G. superba using callus derived from non-dormant corm buds [ 148 ]. Medium supplemented with a combination of plant growth regulators such as BAP (1.5 mg/L), NAA (0.6 mg/L), and polyamine putrescine (15 mg/L) as secondary messengers in signaling pathways, induced maximum shoot buds (87.5).…”

Section: Resultsmentioning

confidence: 99%

“…Using modern biotechnology, Sivakumar et al (2019) reached concentrations of colchicine (2.65 µg/mL, 3.56 µg/mL, and 5.69 µg/mL) within the methanolic extract of in vitro treated leaf samples with spermidine, spermine, and putrescine, resp. [ 148 ]. The obtained amount of colchicine is much higher when compared to the leaf samples from field-grown plants (2.41 µg/mL).…”

Section: Resultsmentioning

confidence: 99%

Anticancer Secondary Metabolites: From Ethnopharmacology and Identification in Native Complexes to Biotechnological Studies in Species of Genus Astragalus L. and Gloriosa L.

Йонкова

Shkondrov

Zarev

et al. 2022

CIMB

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Some of the most effective anticancer compounds are still derived from plants since the chemical synthesis of chiral molecules is not economically efficient. Rapid discovery of lead compounds with pronounced biological activity is essential for the successful development of novel drug candidates. This work aims to present the chemical diversity of antitumor bioactive compounds and biotechnological approaches as alternative production and sustainable plant biodiversity conservation. Astragalus spp., (Fabaceae) and Gloriosa spp. (Liliaceae) are selected as research objects within this review because they are known for their anticancer activity, because they represent two of the largest families respectively in dicots and monocots, and also because many of the medicinally important plants are rare and endangered. We summarized the ethnobotanical data concerning their anticancer application, highlighted the diversity of their secondary metabolites possessing anticancer properties such as saponins, flavonoids, and alkaloids, and revealed the potential of the in vitro cultures as an alternative way of their production. Since the natural supply is limited, it is important to explore the possibility of employing plant cell or organ in vitro cultures for the biotechnological production of these compounds as an alternative.

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“…The accumulation of PAs in plants exposed to environmental stresses and its vital role in stress tolerance have motivated researchers to apply PAs exogenously to cope with the adverse effects of various environmental stresses, such as drought 17 . Several reports have con rmed the role of PAs as a regulatory factor in improving the drought tolerance in medicinal plants, such as chamomile, thyme, fennel, and ame lily [17][18][19][20] . So far, there is no report on the effects of co-application of PAs and AMF on EO content and EO components in cumin plants under drought stress.…”

Section: Introductionmentioning

confidence: 99%

Co-application of arbuscular mycorrhizae fungi and putrescine improves essential oil production and drought tolerance cumin (Cuminum cyminum L.)

Anjam,

Abdipour,

Hosseinifarahi

2024

Preprint

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The effects of arbuscular mycorrhizae fungi (AMF), putrescine (Put), and their combination on essential oil (EO) content, chlorophyll (Chl), carotenoid and EO chemical compounds of cumin (Cuminum cyminum L.) were studied under optimal and drought stress conditions. The foliar application of Put or inoculation with AMF significantly (P ˂ 0.05) increased seed yield, EO content, EO yield, Chl a, Chl b, total Chl, and carotenoid. Moreover, AMF or Put application increased cuminaldehyde, limone, and β-Pinene and decreased γ-Terpinene, o-Cymene, and linalool concentration in cumin plants compared to non-treated plants in both optimal and drought stress conditions. However, the highest EO content, EO yield, Chl a, Chl b, total Chl, carotenoid, and major EO chemical components were obtained when AMF and Put were used together under optimal and drought stress conditions. This study showed that Put and AMF can be co-applied to the cumin plant to significantly improve drought tolerance in field conditions greatly.

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Influence of exogenous polyamines and plant growth regulators on high frequency in vitro mass propagation of Gloriosa superba L. and its colchicine content

Cited by 17 publications

References 50 publications

Micropropagation of Ilex cassine ‘angustifolia’

Micropropagation of Ilex cassine ‘angustifolia’

Anticancer Secondary Metabolites: From Ethnopharmacology and Identification in Native Complexes to Biotechnological Studies in Species of Genus Astragalus L. and Gloriosa L.

Co-application of arbuscular mycorrhizae fungi and putrescine improves essential oil production and drought tolerance cumin (Cuminum cyminum L.)

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