Influence of Experimental Liver Injury on Rat Blood and Alveolar Bone under Stress

Abstract

Experimental liver injury was induced to test rats with a daily injection of thioacetamide (ThAA). The doses used for intraperitoneal administrations were 50 mg/kg body weight. The loss of body weight in the 3-week test period was 15%. In the liver there was seen progressive changes which displayed cell necrosis and regeneration. The influence of ThAA on rat blood and serum was checked using standard biochemical assays consisting of the percentage of blood obtainable and the serum/blood ratio and analysis of serum alanine transferase, serum alkaline phosphatase, serum creatinine, and hydroxyproline in the acute, subacute, chronic, and necrotic stage of liver injury. With ThAA injections, the stimulated rate of glycosaminoglycan synthesis had its association to the serum calcium content. It decreased continuously as function of traumatization time. In the 3-week test period, histological findings show in the alveolar bone, around the teeth, when under occlusal stress and ThAA traumatization, the distinct decrease in osteoblastic activity and less osteoids indicating thus the decreased formation of new bone. Conspicuous osteoclastic resorption was also seen in the same area.

Alizarin Red S-Stained Bone and Cartilage in Calcium Deficiency Provoked by Experimental Liver Injury in Rats

“…and they were similar to those previ ously reported by Lassila and Virtanen for [1984], The kidney sections revealed increased glomerular atrophy with the duration of liver traumatization. An apparent reduction of kidney size was also seen, and the kidney parenchyma was heterogeneously stained by ARS.…”

“…The development of the rat body weight under ex perimental liver traumatization with ThAA was princi pally as expected in accordance with our previous study [Lassila and Virtanen, 1984]. A distinct correlation was noticed between sex and accelerated body weight loss.…”

“…Liver injury, experimentally produced with daily injected thioacetamide (ThAA) doses has several known symptoms [Gressner et al, 1977a[Gressner et al, , b, 1980[Gressner et al, , 1982Lassila and Virtanen, 1984). The main symptom is the marked increase in the synthesis rate of acid glycosaminoglycans (GAG) [Wohlgemut, 1969;Gressner et al, 1977aGressner et al, , b, 1980Gressner et al, , 1982.…”

“…The main symptom is the marked increase in the synthesis rate of acid glycosaminoglycans (GAG) [Wohlgemut, 1969;Gressner et al, 1977aGressner et al, , b, 1980Gressner et al, , 1982. The synthesis rate of derma tan sulfate is exceptionally diminished [Gressner et al, 1980], The increased synthesis rate of GAG produces a gradual calcium deficiency observed both in biochemical [Rasmussen and Bordier, 1974] and experimental studies with adult male rats [Lassila and Virtanen, 1984].…”

“…As a consequence of the sustained calcium deficiency caused by ThAA traumatization increased osteoclastic bone resorption in the alveolar process under occlusal stress was reported [ Lassila and Virtanen, 1984]. The experiment was carried out with adult male rats.…”