Influence of extracellular matrix proteins in enhancing bacterial adhesion to titanium surfaces

Mahmoud, Hanan M.; Williams, David Wynne; Hannigan, Ailish; Lynch, Christopher D.

doi:10.1002/jbm.b.32698

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…On this note, the coating of titanium surfaces with laminin‐5 has been shown to promote epithelial cell adhesion . It has been previously shown that P. gingivalis interfered with oral keratinocyte cell adhesion and spreading on plastic surfaces coated with laminin‐5, and that coating of titanium disks with laminin promoted adhesion of P. gingivalis . Herein, we confirmed that P. gingivalis (the reference strain and the two included clinical isolates) clearly prevented epithelial cell adhesion on titanium–zirconia disks coated with laminin‐5.…”

Section: Discussionsupporting

confidence: 80%

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

et al. 2019

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The study investigated in vitro the effect of Porphyromonas gingivalis and its cysteine proteases (gingipains) on epithelial cell adhesion to titanium–zirconium alloy surfaces. Titanium–zirconium discs with a standard machined (M) or chemically modified hydrophilic surface (modM) were coated with lamin‐5 and incubated with telomerase‐inactivated gingival keratinocytes (TIGK). Three P. gingivalis strains or gingipains were either added simultaneously with TIGK or after TIGK cells were already attached to the disks. Adhered TIGK cells were counted at 24 h. All P. gingivalis strains clearly inhibited adhesion of TIGK cells to M and modM surfaces. Compared with bacteria/gingipain‐free TIGK cell cultures, the number of attached TIGK cells was reduced by about 80% and 60% when P. gingivalis was added simultaneously or after TIGK cells were already attached to the disks (each p < 0.01), respectively. Counts of attached cells were similarly reduced when only gingipains were used. Adhesion molecules of TIGK cells, in particular E‐cadherin, were cleaved by P. gingivalis. In conclusion, P. gingivalis and gingipains interfere with the adhesion of epithelial cells to titanium–zirconium alloy surfaces by cleaving adhesion molecules, while a chemically modified hydrophilic titanium–zirconium alloy surface did not yield any protection. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2549–2556, 2019.

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Section: Discussionsupporting

confidence: 80%

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

et al. 2019

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“…This nevertheless indicates that the presence of IgG layer on the films already alleviates the remarkable impact of the special functionalization of the surface (surface grafting). In fact, the enhanced bacteria adhesion as a result of the preadsorption of IgG is not surprising, since conditioning layer (IgG layer in this case) usually promotes formation of bacterial biofilm . Many other serum protein layers, such as collagen, fibronectin and vitronectin, were reported to have similar effect.…”

Section: Resultsmentioning

confidence: 92%

“…In fact, the enhanced bacteria adhesion as a result of the preadsorption of IgG is not surprising, since conditioning layer (IgG layer in this case) usually promotes formation of bacterial biofilm . Many other serum protein layers, such as collagen, fibronectin and vitronectin, were reported to have similar effect. It was found that S. epidermidis could specifically bind Fbe sites to fibrinogen, SdrG to collagen, autolysins AtlE and Aae to vitronectin, in turn accelerating the formation of bacterial biofilm …”

Section: Resultsmentioning

confidence: 92%

Adsorption‐associated orientational changes of immunoglobulin G and regulated phagocytosis ofStaphylococcus epidermidis

Hou

Liu

Zhang

et al. 2018

J Biomedical Materials Res

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Understanding the adsorption of immunoglobulin G (IgG) on biomaterials surfaces is crucial for design and modification of the surfaces to alleviate inflammatory responses after implantation. Here, we report direct visualization and two-dimensional (2D) image interpretation of the IgG molecule adsorbed on simplified surfaces by single particle electron microscopy and atomic force microscopy. Influence of the orientational changes in adsorbed IgG on phagocytosis of macrophages against Staphylococcus epidermidis is further examined. Untreated amorphous carbon film and -COOH and -NH grafted carbon films are employed as the model surfaces for the adsorption testing. Results show that IgG displays flat orientation lying on the untreated surface, while forms vertical orientations standing on the functionalized surfaces. These specific spatial alignments are associated with altered unfolding extent and structure rearrangement of IgG domains, which are influenced synergistically by surface charge and wettability of the substrata. The changes in interdomain distance of IgG molecules subsequently regulate immune behaviors of macrophages and phagocytosis of S. epidermidis. The results would give insight into appropriate design of biomaterial surfaces in nanoscales for desired inflammatory responses. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2838-2849, 2018.

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“…Given that bacterial adhesion is a complex process, many research efforts have been devoted to clarifying the phenomenon from two possible perspectives: on the one hand, it is believed that the effect of adsorbed proteins on bacterial adhesion and biofilm formation is determined by surface physicochemical properties including wettability, roughness, and charge state, − which could be altered by the protein adsorption process. On the other hand, some researchers believe that the specific molecular recognition between adsorbed proteins and bacterial cell wall receptors − plays the decisive role, thus the conformation of the adsorbed proteins was the key point. In this work, we analyzed the relationship between the altered surface physicochemical properties and bacterial adhesion and biofilm formation of S. epidermids and E. coli .…”

Section: Resultsmentioning

confidence: 99%

Preadsorption of Serum Proteins Regulates Bacterial Infections and Subsequent Macrophage Phagocytosis on Biomaterial Surfaces

Hou

Liu

et al. 2019

ACS Appl. Bio Mater.

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Bacterial infection has been one of the main obstacles for extensive biomedical applications of biomaterial films. Understanding the interactions among macromolecules, cells, and bacteria in the microenvironment located on the film surface at the molecular level is essential for developing antibacterial films. Here we report the distinct influence of several key serum proteins adsorbed on diamond-like carbon (DLC) and traditional Ti films on initial bacterial adhesion, biofilm formation, and corresponding immune responses. Type I collagen, Fn, and IgG were selected as the typical serum proteins. Gram-positive bacterium Staphylococcus epidermidis and Gram-negative bacterium Escherichia coli were used as the model bacteria. Macrophage phagocytosis tests were carried out to examine the impact of adsorbed proteins on the ability of macrophages to clear the adhered pathogens. Results show that it was the specific molecular recognition between adsorbed proteins and bacteria, not the surface physiochemical properties such as surface wettability, surface roughness, and surfaces charge, that decisively affected bacterial adhesion and following biofilm formation. Collagen resisted bacterial adhesion on both DLC and Ti films, even though the molecules exhibited distinct conformations on the two surfaces, whereas for Fn and IgG, the specific molecular recognition was closely related to protein conformations. Fn molecules formed globular aggregates on Ti surfaces that greatly enhanced bacterial adhesion but exhibited a fibril conformation on DLC surfaces that inhibited bacterial adhesion. IgG showed an end-on orientation with free F(ab) 2 domains on Ti surfaces, facilitating bacterial adhesion and biofilm formation, while the flattened orientation on DLC films showed little effect on bacterial behaviors. Furthermore, preadsorption of Fn and IgG significantly promoted the phagocytosis ability of macrophages against S. epidermidis and affected the corresponding secretion of inflammatory cytokine. These results would give insights into understanding protein−surface interactions for developing appropriate surface modification techniques for biomaterials with desired anti-inflammatory functions.

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Influence of extracellular matrix proteins in enhancing bacterial adhesion to titanium surfaces

Abstract: ECM matrix proteins promote adherence of potential periodontal pathogenic bacteria to titanium surfaces. Management of diseases such as peri-implantitis should focus on limitation of this bacterial adherence to reduce/eliminate biofilms.

Cited by 9 publications

References 30 publications

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

Gingipains impair attachment of epithelial cell to dental titanium abutment surfaces

Adsorption‐associated orientational changes of immunoglobulin G and regulated phagocytosis ofStaphylococcus epidermidis

Preadsorption of Serum Proteins Regulates Bacterial Infections and Subsequent Macrophage Phagocytosis on Biomaterial Surfaces

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