Influence of Extracellular Vesicles from the Follicular Fluid of Young Women and Women of Advanced Maternal Age with Different miRNA Profiles on Sperm Functional Properties

Sysoeva, A. P.; Nepsha, O. S.; Makarova, N. P.; Silachev, Denis N.; Lobanova, N. N.; Timofeeva, Timofeeva A.V.; Shevtsova, Yu. A.; Брагина, Е. Е.; Kalinina, Kalinina E.A.

doi:10.1007/s10517-022-05589-x

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…The results presented in this work indicate a trend according to which the level of vesicular progesterone from the FF EVs was notably higher (6.6-fold) in women under 35 years of age. Numerous studies suggested that progesterone in FF EVs plays a key role in attracting sperm to the oocyte and in their hyperactivation and acquisition of fertilizing ability [30][31][32], activating the Ca 2+ dependent channel CatSper; our previous work also confirms these results [1]. Progesterone is the main signal for the release of sperm from the isthmus of the oviduct, known as the functional sperm reservoir, and is the main chemoattractant in humans [33].…”

Section: Discussionsupporting

confidence: 82%

“…This work is part of a large-scale study on the influence and interaction of follicular fluid extracellular vesicles (FF EVs) with human sperm in the female reproductive tract during fertilization. In our previous work, we were the first to demonstrate the peculiarities of the binding of FF EVs from women of different ages to the sperm membrane and we also demonstrated a significant improvement in the characteristics of motility, hyperactivation and membrane state changes necessary for fertilization [1].…”

Section: Introductionmentioning

confidence: 69%

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Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Sysoeva,

Akhmedova,

Nepsha

et al. 2024

Life

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The aim of this study was to investigate the molecular composition of follicular fluid (FF) extracellular vesicles (EVs) in women of different reproductive ages and its possible relationship to sperm fertilizing ability. FF EVs were obtained by differential centrifugation. The concentration and size distribution of FF EVs were analyzed by nanoparticle tracking analysis. The lipidome and proteome were analyzed by liquid chromatography–mass spectrometry. The isolated FF EVs had a variety of shapes and sizes; their concentration and size distribution did not differ significantly between the age groups. In women younger than 35 years, the concentration of vesicular progesterone was 6.6 times higher than in women older than 35 years, and the total levels of the main lipid classes were increased in younger women. A proteomic analysis revealed that not only FF EV-specific proteins, but also proteins involved in sperm activation were present. New data were obtained on the composition of FF EVs, confirming their importance as molecular indicators of age-related changes in the female reproductive system. In addition, these results shed light on the possible interaction between the FF EVs of women in different age groups and male germ cells. Therefore, studying the transcriptomic and metabolomic profile of FF EVs may be a crucial approach to evaluate the efficacy of ART.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 69%

Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Sysoeva,

Akhmedova,

Nepsha

et al. 2024

Life

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“…A specific combination of these small ncRNAs in FF-sEVs could predict pregnancy in a more precise manner. The FF microenvironment is known to reflect the oocyte status and the relationships between FF-EVs and oocyte maturation in human and bovine 25 – 28 , molecular mechanisms of PCOS 29 and age 30 – 32 have been reported in previous studies. A more precise study revealed the peptide profile of biomarkers associated with fertilization outcome 33 .…”

Section: Discussionmentioning

confidence: 84%

Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology

Muraoka,

Yokoi,

Yoshida

et al. 2024

Commun Med

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Background Assisted reproductive technology accounts for an increasing proportion of infertility treatments, and assessments to predict clinical pregnancy outcomes are desired. Extracellular vesicles exist in follicular fluid, and small non coding RNAs in extracellular vesicles underline the possibility of reflecting pregnancy potential. Methods Follicular fluid samples are collected from 20 ovarian follicles of 15 infertile patients undergoing assisted reproductive technology. Extracellular vesicles are isolated by serial centrifugation and small RNA sequencing is performed to investigate the profiles of microRNAs and P-element-induced wimpy testis-interacting RNAs. Results Small extracellular vesicles with a size range of approximately 100 nm are successfully isolated, and the small non coding RNA profiles of pregnant samples (n = 8) are different from those of non-pregnant samples (n = 12). Fourteen dysregulated small non coding RNAs are selected to identify the independent candidates [mean read count >100, area under the curve >0.8]. Among them, we find that a specific combination of small non coding RNAs (miR-16-2-3p, miR-378a-3p, and miR-483-5p) can predict the pregnant samples more precisely using a receiver operating characteristics curves analysis (area under the curve: 0.96). Furthermore, even in the same patients, the three microRNAs are differentially expressed between pregnant and non-pregnant samples. Conclusions Our results demonstrate that small non coding RNAs derived from small extracellular vesicles in follicular fluid can be potential non-invasive biomarkers for predicting pregnancy, leading to their probable application in assisted reproductive technology. Further large-scale studies are required to validate the clinical usefulness of these small non coding RNAs.

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Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro

Nepsha,

Burmenskaya,

Akhmedova

et al. 2024

Bull Exp Biol Med

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Influence of Extracellular Vesicles from the Follicular Fluid of Young Women and Women of Advanced Maternal Age with Different miRNA Profiles on Sperm Functional Properties

Cited by 4 publications

References 28 publications

Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology

Changes in the Transcription of Proliferation- and Apoptosis-Related Genes in Embryos in Women of Different Ages under the Influence of Extracellular Vesicles from Donor Follicular Fluid In Vitro

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