Influence of extract derived cell cultures of broccoli against osteoporosis in ovariectomized rats

Abstract

This study was conducted to enhance broccoli secondary metabolites; using plant biotechnology techniques and tissue culture as well as to evaluate the therapeutic effect of broccoli extract against osteoporosis in ovariectomized (OVX) rats. Cell culture technique was implemented in order to achieve and produce polyphenols from leaf explants of broccoli. The highperformance liquid chromatography (HPLC) technique was used to evaluate the polyphenolic profiles of ethanolic extracts for cell cultures compared to the dried inflorescences of broccoli (cultivated plant). However, total phenol and flavonoid contents were determined by spectrophotometric technique. Induction of osteoporosis was performed via ovarectomy operation where rats were divided into 5 groups (10 rats each). Group 1: Sham operated control group and the remaining rats were ovariectomized for 12 weeks to produce osteoporosis model. Group 2: Ovariectomized osteoporotic positive control group. Groups 3-5: Ovariectomized rat daily received 17-β-estradiol (10 µg/kg), broccoli cell suspension culture ethanol extracts (300 & 600 mg/kg) orally. The obtained results showed that Murashige and Skoog basal nutrient medium (MS) fortified with 2.25 µM of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.55 µM of kinetin (kin.) showed the best medium for calli production. Addition of methionine enhanced polyphenolic production in cell suspension culture which was identified by HPLC. Administration of broccoli cell suspension extract elevated the uterine weight, high density lipoprotein-cholesterol and estradiol 2 (E2) serum levels and reduced serum levels of lipid profile, liver and kidney functions, osteocalcin and total procollagen type 1 N-terminal propeptide (TPINP). In conclusion, broccoli may be a promising source of secondary metabolites; polyphenol contents and flavonoids for treatment of bone loss and cell damage in osteoporosis.

Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition

“…The animals were observed for mortality 24 h later. Additionally, mice were observed for any changes in the skin, fur, respiratory, circulatory, or behavioral pattern [20,24]. 2.6.3.…”

“…The targets of the current work were established based upon the significant antioxidant and cytotoxic activity of the EO and extracts of different parts of A. bidiwillii [1], and the potent anti-inflammatory and antipyretic potentialities of EO of A. heterophylla [20,24]. Additionally, the previous documents indicated the potential role of the nanoforms of the different plant extracts in the modification of the biological potentialities of these extracts [20,24].…”

“…The targets of the current work were established based upon the significant antioxidant and cytotoxic activity of the EO and extracts of different parts of A. bidiwillii [1], and the potent anti-inflammatory and antipyretic potentialities of EO of A. heterophylla [20,24]. Additionally, the previous documents indicated the potential role of the nanoforms of the different plant extracts in the modification of the biological potentialities of these extracts [20,24]. Thus, the main outlines of current work were (i) identification of the chemical composition of the tree shoots, A. bidiwillii, (ABSEO) cultivated in Egypt, (ii) formulation of the extracted of EO in nanoemulsion form, (iii) evaluation of the oral and topical antiinflammatory as well as antipyretic potentialities of EO and its nanoemulsion depending upon biochemical, pathological and immunohistochemical analysis…”

Cytoprotective potentialities of carvacrol and its nanoemulsion against cisplatin-induced nephrotoxicity in rats: development of nano-encasulation form

“…The blood urea and serum creatinine were determined via kits (Spectrum chemical company, New Brunswick, USA) according to the kinetic method as mentioned by Salama et al. [ 39 ].…”

Hypolipidemic Effects Of Red Radish (Raphanus Sativus) Seed Oil In Rat Fed High-Fat Diet: Its Phytochemical Characterization

“…Blood samples were collected every two weeks in heparinized tubes withdrawn from the retro-orbital venous plexus under local anesthesia by diethyl ether and immediately mixed with ethylene diamine tetra acetic acid (EDTA) as anticoagulant. Blood samples were centrifuged at 4000 rpm for 20 min at 4 o C [45] to obtain plasma for the determination of cholesterol, triglycerides, total lipids, HDL and LDL using kits from Salucea Company, the Netherlands. Body weight of rats was recorded every two weeks.…”