Influence of extractants and filter materials in the extraction of dissolved organic matter (DOM) from subtropical agricultural soil

Zheng, Xiangbi Chen Xiaodong; Liang, Yajun Hu Chengming; Wu, Yirong Su Jinshui

doi:10.9755/ejfa.2018.v30.i3.1635

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…Given that soil C accessibility is largely associated with mineral protection, we used three types of proxies (DOC, unprotected C and mineral‐protected C) to explore the changes in soil C accessibility. To be specific, DOC of fresh soil was extracted with deionized water at a soil: water ratio of 1:5 to represent the accessible C in field pore water (Zheng et al, 2018). The soil extract was shaken at room temperature for 24 h, centrifuged and filtered through a 0.45‐μm polyethersulphone membrane.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen input enhances microbial carbon use efficiency by altering plant–microbe–mineral interactions

Qin

Zhang

Chen

et al. 2022

Global Change Biology

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Microbial growth and respiration are at the core of the soil carbon (C) cycle, as these microbial physiological performances ultimately determine the fate of soil C. Microbial C use efficiency (CUE), a critical metric to characterize the partitioning of C between microbial growth and respiration, thus controls the sign and magnitude of soil C-climate feedback. Despite its importance, the response of CUE to nitrogen (N)

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Section: Methodsmentioning

confidence: 99%

Nitrogen input enhances microbial carbon use efficiency by altering plant–microbe–mineral interactions

Qin

Zhang

Chen

et al. 2022

Global Change Biology

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“…We also collected a soil extract and a surface water sample that contained DOM. The soil extract was collected as the supernatant of a suspension prepared by adding buffer to a characterized silty clay loam soil (Table S4) as described in Section S3.1. , The surface water sample was collected from the Missouri River near St. Charles, Missouri, and filtered with a 0.22 μm poly(ether sulfone) membrane. Characterized soil extract and river water quality parameters are reported in Table S4 and Table S5, respectively.…”

Section: Methodsmentioning

confidence: 99%

Binding of Dissolved Organic Matter to RNA and Protection from Nuclease-Mediated Degradation

Chatterjee,

Zhang,

Parker

2023

Environ. Sci. Technol.

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The persistence of RNA in environmental systems is an important parameter for emerging applications, including ecological surveys, wastewater-based epidemiology, and RNA interference biopesticides. RNA persistence is controlled by its rate of biodegradation, particularly by extracellular enzymes, although the specific factors determining this rate have not been characterized. Due to prior work suggesting that nucleic acids−specifically DNA−interact with dissolved organic matter (DOM), we hypothesized that DOM may bind RNA and impede its biodegradation in natural systems. We first adapted a technique previously used to assess RNA-protein binding to differentiate RNA that is bound at all sites by DOM from RNA that is unbound or partially bound by DOM. Results from this technique suggested that humic acids bound RNA more extensively than fulvic acids. At concentrations of 8−10 mg C /L, humic acids were also found to be more effective than fulvic acids at suppressing enzymatic degradation of RNA. In surface water and soil extract containing DOM, RNA degradation was suppressed by 39−46% relative to pH-adjusted controls. Due to the ability of DOM to both bind and suppress the enzymatic degradation of RNA, RNA biodegradation may be slowed in environmental systems with high DOM concentrations, which may increase its persistence.

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Mineral and Microbial Properties Drive the Formation of Mineral‐Associated Organic Matter and Its Response to Increased Temperature

Zhao,

Feng,

et al. 2024

Global Change Biology

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A comprehensive understanding of the formation of mineral‐associated organic matter (MAOM) is a prerequisite for the sustainable management of soil carbon (C) and the development of effective long‐term strategies for C sequestration in soils. Nevertheless, the precise manner by which microbial and mineral properties drive MAOM formation efficiency and its subsequent response to elevated temperature at the regional scale remains unclear. Here, we employed isotopically labelled laboratory incubations (at 15°C and 25°C) with soil samples from a ~3000 km transect across the Tibetan Plateau to elucidate the mechanisms underlying MAOM formation and its temperature response. The results indicated that both mineral protection and microbial properties were critical predictors of MAOM formation across the geographic gradient. The efficiency of MAOM formation was found to increase with the content of iron (Fe) oxides and their reactivity [i.e., the ratio of poorly crystalline Fe oxides to total Fe oxides (Feo:Fed)] but to decrease with the relative abundance of Gammaproteobacteria and Actinobacteria across the plateau. Moreover, a notable decline in MAOM formation efficiency was observed under elevated temperatures, which was concomitant with a reduction in the content and reactivity of Fe oxides, as well as the microbial assimilation of the labelled substrate. The attenuation of mineral–organic associations was identified as the primary factor contributing to the warming‐induced reduction in MAOM formation. These findings highlight the necessity of incorporating organo–mineral associations and microbial properties into Earth System Models to accurately project soil C dynamics under changing climate.

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Influence of extractants and filter materials in the extraction of dissolved organic matter (DOM) from subtropical agricultural soil

Cited by 3 publications

References 40 publications

Nitrogen input enhances microbial carbon use efficiency by altering plant–microbe–mineral interactions

Nitrogen input enhances microbial carbon use efficiency by altering plant–microbe–mineral interactions

Binding of Dissolved Organic Matter to RNA and Protection from Nuclease-Mediated Degradation

Mineral and Microbial Properties Drive the Formation of Mineral‐Associated Organic Matter and Its Response to Increased Temperature

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