Mushroom (Flammulina velutipes) stem residue (MSR) is rich with bioactive ingredients. This study aimed to assess the role of mushroom stem residue (MSR) on growth performance, meat quality, antioxidant status, and lipid metabolism of broilers. A total 168 Arbour Acres male broilers were assigned into 3 treatments (7 replications, 8 chicks each) and fed a basal diet as control (CON); 1% mushroom stem residue containing diet (1% MSR); and 2% MSR containing diet. Superoxide dismutase, glutathione peroxidase, and catalase value in serum, thigh muscle, breast muscle and liver sample, were higher (linear and quadratic, p < .05) in MSR groups than that of CON. Malondialdehyde content in serum, thigh muscle, breast muscle and liver tissue sample, were lower (linear and quadratic, p < .05) in MSR diets than CON. Compared with control, total cholesterol value of liver sample was lower (linear and quadratic, p < .05) in 2% MSR diet on day 21 and day 42. Triglyceride value was also lower (linear and quadratic, p < .05) in both levels of MSR diets than control on day 21 and day 42. MSR at 2% level can be applied on improving antioxidant status and lipid metabolism of broilers. HIGHLIGHTS Flammulina velutipes mushroom stem residue (MSR) contains different bio-active ingredients which can be used in broiler diets. MSR had no any adverse effects on growth performance and normal meat quality of broilers. MSR could improve different antioxidant enzyme activities in breast muscle, thigh muscle, liver tissue and serum of broilers. MSR could reduce cholesterol levels in liver tissue of broilers. MSR at 2% level can be applied on improving antioxidant status and lipid metabolism in broilers.