Influence of fish size and water temperature on the metabolic demand for oxygen by barramundi, Lates calcarifer (Bloch), in freshwater

Glencross, Brett; Felsing, M.

doi:10.1111/j.1365-2109.2006.01526.x

Cited by 22 publications

(22 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As poikilotherms, fishes are an ideal organism in which to study the effects of temperature and stress as their metabolic rate is strictly dependent on the temperature of their environment, however, the majority of studies focusing upon gene regulation do so in temperate fishes and until now gene expression studies on tropical fishes under stress have been few (Buentello et al , 2000; Mallekh & Lagardere et al , 2002; Glencross & Felsing et al , 2006). Barramundi Lates calcarifer (Bloch 1790) are an ideal species in which to study the gene expression response to an acute heat stress of a tropical estuarine species.…”

Section: Introductionmentioning

confidence: 99%

The gene expression response of the catadromous perciform barramundi Lates calcarifer to an acute heat stress

Newton

Santis

Jerry

2012

Journal of Fish Biology

View full text Add to dashboard Cite

The acute heat-shock response of the tropical estuarine fish species barramundi Lates calcarifer as indicated by the expression of genes within stress (hsp 90AA, hsp 90AB, hsp 70 and hsc 70), metabolic (cisy, cco II and ldh) and growth (igf1 and mstn 1) related pathways was examined following an increase in water temperature from 28 to 36° C over 30 min. Lates calcarifer were maintained at the acute stress temperature of 36° C for 1 h before being returned to 28° C and allowed to recover at this temperature for a further 2 weeks. Muscle tissue sampling over the experimental period allowed for the expression quantification of stress, metabolic and growth-related genes via quantitative real-time polymerase chain reaction (qrt-PCR) where a robust and reliable normalization approach identified both α-tub and Rpl8 as appropriate genes for the analysis of gene expression in response to an acute heat stress. hsp90AA and hsp70 of the inducible heat-shock response pathway showed a massive up-regulation of gene expression in response to heat stress, whilst the constitutive heat-shock genes hsp90AB and hsp70 showed no change over the course of the experiment and a small increase after 2 weeks of recovery, respectively. Of the three genes representing the metabolic pathway (cisy, cco II and ldh) only cco II changed significantly showing a decrease in gene expression, which may suggest a small suppression of aerobic metabolism. igf1 of the growth pathway showed no significant differences in response to an acute heat stress, whilst mstn1 increased at the beginning of the heat stress but returned to basal levels soon after. Overall, the results demonstrate that an acute heat stress in L. calcarifer caused a significant increase in the expression of genes from the stress response pathway and a possible decrease in aerobic metabolism with only relatively minor changes to the growth pathway highlighting the hardy nature of L. calcarifer and its resilience in coping with sudden temperature changes routinely encountered within its natural environment.

show abstract

Section: Introductionmentioning

confidence: 99%

The gene expression response of the catadromous perciform barramundi Lates calcarifer to an acute heat stress

Newton

Santis

Jerry

2012

Journal of Fish Biology

View full text Add to dashboard Cite

show abstract

“…At these parameters the standard metabolic rate of S. rivulatus estimated in the present work was 0.57 ± 0.02 mg O 2 g −1 h −1 (mean ± SE). However, it is noteworthy that changes in temperature (Glencross & Felsing ) and salinity affect standard metabolic rate (Varsamos et al . ) and a new SMR should be measured if environmental conditions change with season or location.…”

Section: Discussionmentioning

confidence: 99%

Effects of salinity on standard metabolic rate of juvenile marbled spinefoot ( Siganus rivulatus )

2016

View full text Add to dashboard Cite

Marbled spinefoot, Siganus rivulatus, is a herbivorous euryhaline teleost widely distributed in the Eastern Mediterranean. It is an economically valuable species and a suitable candidate for warm water aquaculture. Accordingly, understanding the effects of environmental factors on fish metabolism is important to optimize culture conditions. Two experiments were performed to establish standard metabolic rate and study the effect of salinity on metabolism of marbled spinefoot. In the first experiment, a series of flow-through respirometry experiments was performed at 27°C and 35 g L À1 .The standard metabolic rate of marbled spinefoot juveniles was calculated as 0.57 AE 0.02 mg O 2 g À1 h À1 (mean AE SE). In the second experiment, fish were maintained at salinities of 25, 30, 35 and 40 g L À1 for 2 weeks. Flow-through respirometry was performed to measure respiration rates at the various salinities. Respiration rates were similar among fish in salinities of 30, 35 and 40 g L À1 but increased significantly at 25 g L À1 . Results suggest that despite the euryhalinity of marbled spinefoot, farmers should maintain salinity within the optimal range of 30-40 g L À1 in order to improve productivity.

show abstract

“…Had we had the opportunity to study Moapa dace in its historic range in the Muddy River, where waters are cooler, the influence of temperature in our models would likely be greater because larger, older fishes frequently inhabit cooler water [45], [46]; a phenomena previously observed in Moapa dace [12]. A reduction in springflows on the Refuge or Muddy River could result in stream cooling [47], which may reduce the area currently suitable for rearing, foraging, and spawning (26°−32°C).…”

Section: Discussionmentioning

confidence: 99%

An Ecohydraulic Model to Identify and Monitor Moapa Dace Habitat

et al. 2013

View full text Add to dashboard Cite

Moapa dace (Moapa coriacea) is a critically endangered thermophilic minnow native to the Muddy River ecosystem in southeastern Nevada, USA. Restricted to temperatures between 26.0 and 32.0°C, these fish are constrained to the upper two km of the Muddy River and several small tributaries fed by warm springs. Habitat alterations, nonnative species invasion, and water withdrawals during the 20th century resulted in a drastic decline in the dace population and in 1979 the Moapa Valley National Wildlife Refuge (Refuge) was created to protect them. The goal of our study was to determine the potential effects of reduced surface flows that might result from groundwater pumping or water diversions on Moapa dace habitat inside the Refuge. We accomplished our goal in several steps. First, we conducted snorkel surveys to determine the locations of Moapa dace on three warm-spring tributaries of the Muddy River. Second, we conducted hydraulic simulations over a range of flows with a two-dimensional hydrodynamic model. Third, we developed a set of Moapa dace habitat models with logistic regression and a geographic information system. Fourth, we estimated Moapa dace habitat over a range of flows (plus or minus 30% of base flow). Our spatially explicit habitat models achieved classification accuracies between 85% and 91%, depending on the snorkel survey and creek. Water depth was the most significant covariate in our models, followed by substrate, Froude number, velocity, and water temperature. Hydraulic simulations showed 2–11% gains in dace habitat when flows were increased by 30%, and 8–32% losses when flows were reduced by 30%. To ensure the health and survival of Moapa dace and the Muddy River ecosystem, groundwater and surface-water withdrawals and diversions need to be carefully monitored, while fully implementing a proactive conservation strategy.

show abstract

Influence of fish size and water temperature on the metabolic demand for oxygen by barramundi, Lates calcarifer (Bloch), in freshwater

Cited by 22 publications

References 13 publications

The gene expression response of the catadromous perciform barramundi Lates calcarifer to an acute heat stress

The gene expression response of the catadromous perciform barramundi Lates calcarifer to an acute heat stress

Effects of salinity on standard metabolic rate of juvenile marbled spinefoot ( Siganus rivulatus )

An Ecohydraulic Model to Identify and Monitor Moapa Dace Habitat

Contact Info

Product

Resources

About