Influence of fish skin gelatin–sodium alginate complex stabilized emulsion on benzyl isothiocyanate stability and digestibilityin vitroandin vivo

Abstract

BACKGROUND: An emulsion delivery system for benzyl isothiocyanate (BITC) was prepared using fish skin gelatin (FSG) and sodium alginate (Alg). The effects of the FSG-Alg complex on the emulsion stability and BITC release pattern from the emulsion were investigated in vitro and in vivo. RESULTS:The storage stability and embedding rate of the 10 g kg −1 FSG and 2.5 g kg −1 Alg (FSG-Alg) emulsion were the highest among all samples. The FSG-Alg complex provided BITC a better protection during in vitro digestion. The microstructure of the FSG-Alg emulsions was more stable during in vitro digestion, and the bioaccessibility and retention rate of BITC were much higher compared to those of the FSG emulsion. The results of the ex vivo everted gut sac of rat intestine study showed that the FSG-Alg emulsion significantly increased the BITC absorption rate in the duodenum.CONCLUSION: The FSG-Alg emulsion delivery system is a highly stable system for the delivery of BITC that improves the bioaccessibility of BITC and promotes its absorption in the duodenum.

Construction of benzyl isothiocyanate‐loaded fish skin gelatin‐luteolin compound emulsion delivery system, and its digestion and absorption characteristics

“…Each sac was placed into 7 mL emulsion/BITC oil solution after digestion at 37 °C and continuously aerated with 5% CO 2 and 95% O 2 for 120 min. Samples from the serosal side were collected separately and analyzed using HPLC 21 …”

“…The BITC embedding rates in both emulsions were determined using an LC-20 AD high-performance liquid chromatography (HPLC) system (Shimadzu Corp, Kyoto, Japan) and a kromasil 100-5 ODS column (250 mm × 4.6 mm, 5 μm; AkzoNobel Corp, Sweden) was used to identify the BITC. 21 In brief, unembedded BITC was removed by mixing 1 mL n-hexane with 1 mL emulsion. The emulsion was mixed with 1 mL methanol to precipitate protein, and an equal volume of n-hexane solution was added.…”

The stability and reactivity of isothiocyanates and the plausible behavior of their dithiocarbamate- and thiourea-conjugates

“…In addition, the addition of L-cysteine (CYS) effectively prevented BITC from decomposition during 4 °C storage, and the amounts of BITC were maintained during storage for 14 days [5]. In contrast, N- alginate complex, to increase its stability and bioaccessibility [8,9]. Since the poor water solubility, low bioaccessibility, and basic unsteadiness of BITC limit the application of BITC in the food industry, the fish protein-based emulsion system is one of the promising strategies to protect and deliver unstable ITCs due to their non-toxicity, low cost and good accessibility.…”