Influence of Flavonoids on the Cytotoxic Activity of Mononuclear Blood Cells in Model Tests

Abstract

BACKGROUND: The spread of phytocomplex application and justification of its selective effects on tumour cells (mainly due to the presence of flavonoids) require research of its cytotoxic and immunomodulatory activity.
 AIM: The goal was to study the direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of the donor's mononuclear blood cells in in vitro experiments.
 METHODS: The phytocomplex was a dry extract from marsh cinquefoil, creeping alfalfa and common hop; its main active ingredients were flavonoids. Transplantable monolayer cultures of lung adenocarcinoma, colorectal cancer, erythroblastic leukaemia, and fibroblasts were used as target cells. The cytotoxic activity was assessed using a cytotoxic test based on the selective ability to live cells to reduce MTT (3-[4, 5-dimethyltriazol-2-yl]-2, 5 diphenyltetrazolium bromide) to formazan in mitochondria. Quantitative determination of formazan was performed using spectrophotometry.
 RESULTS: A direct cytotoxic effect of the phytocomplex in concentrations of at least 2.5 mg/ml on tumour cells has been established. Its modulating effect on the cytotoxic activity of mononuclear blood cells at a concentration of 0.05 mg/ml was shown. The phytocomplex in doses of 0.25 and 0.5 mg/ml increased the killer activity of the mononuclear cells in a diseased person's blood, but did not affect these blood cells in a healthy donor. Incubation of lymphocytes with a phytocomplex for 24 hours increased the cytotoxic activity of mononuclear cells by 20-25%.
 CONCLUSION: The direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of mononuclear blood cells in model experiments in vitro have been established.

Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation

“…Therefore, AMH was further fractionated by column chromatography (CC) and purified by HPLC to afford six new flavonoid glycosides, named as mongoflavonosides A 1 -A 4 (1-4), B 1 (5), B 2 (6), four new phenolic acid glycosides, named as mongophenosides A 1 -A 3 (7)(8)(9), B (10) (Figure 1), along with known compounds, kaempferol-3,7,4 -tri-O-β-glucoside (18) [19], quercetin-3-O-β-d-rutinoside-7-O-β-d-glucuronide (25) [20], quercetin-3,7,4 -tri-O-glucoside (26) [21], isorhamnetin 3-O-β-d-glucopyranoside (27) [22], as well as three phenols, trans-p-hydroxycinnamate sophorose (28) [23], tuberonoid A (29) [24], trans-caffeic acid (30) [25], and benzyl-O-β-d-glucopyranoside (31) [26] (Figure 2). Among the known isolates, 28 and 30 were obtained from the Allium genus for the first time; 18, 25-27, 29, and 31 were firstly found from the plant.…”

“…Flavonoids and phenolic acids are secondary metabolites found in most Allium vegetables, such as onion (Allium cepa L.) [1], scallion (Allium fistulosum L.) [2], garlic (Allium sativum L.) [3], fruits, and traditional medicine. They also exert multiple biological properties, such as antitumor [4,5] antioxidant [6,7], anti-inflammation and gastrointestinal motility effect improvement [8][9][10][11], which make them show high correlations in the inhibition or management of many chronic diseases, such as cardiovascular and cerebrovascular diseases, diabetes, cancer, digestive system diseases, and so on.…”