Influence of Freeze-Thawing and Storage on Human Serum Lipid Analytes

Ugwuezumba, P.C.; Nwankpa, Promise; Emengaha, F.C.; Ekweogu, C.N.; Etteh, C.C.; Chukwuemeka, O.G.

doi:10.20546/ijcmas.2018.710.381

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Finally, to avoid the in vitro degradation of blood components all samples were frozen at −80 ℃. This temperature has been shown to preserve most of biochemical magnitudes for at least 12 months, including lipid profile parameters (23), however, this information is not available for all markers studied. Serum lipid profile observed in patients cohort were in agreement with studies published on dyslipidemia in ESRD (4,24).…”

Section: Discussionmentioning

confidence: 99%

Biological variation of metabolic cardiovascular risk factors in haemodialysis patients and healthy individuals

Corte¹,

Venta²

2020

Ann Transl Med

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Background: Maintenance haemodialysis (HD) is associated with a very high cardiovascular risk and the assessment of metabolic cardiovascular risk factors is essential for an adequate diagnosis and management.The aim of the study has been to estimate the biological variation (BV) of serum lipids, calcium, inorganic phosphorus, 25-OH vitamin D, C-reactive protein and plasma intact parathyroid hormone (PTHi) and total homocysteine, to evaluate whether HD alter the homeostatic set-point of these magnitudes.Methods: Blood samples were collected from 18 HD patients in steady-state conditions, one per month during 6 months, and from 11 healthy volunteers at weekly intervals over 5 weeks. BV data were derived using CV-ANOVA.Results: Within-subject coefficients of variation (CVI) and derived reference change values (RCV) estimated in HD patients were significantly higher than in healthy individuals for calcium (CVI =3.8% vs.2.3%) (RCV =12.6% vs. 7.6%), inorganic phosphorus (13.1% vs. 8.0%) (RCV =38.7% vs. 30.1%) and PTHi (20.3% vs. 11.3%) (RCV =57.5% vs. 34.0%).Conclusions: For calcium, inorganic phosphorus and PTHi, RCV derived from advanced disease-specific BV data is recommended for the management of bone-mineral disturbances in HD patients. For the rest of cardiovascular risk factors, the use of RCV derived from healthy individuals could be appropriate.

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Section: Discussionmentioning

confidence: 99%

Biological variation of metabolic cardiovascular risk factors in haemodialysis patients and healthy individuals

Corte¹,

Venta²

2020

Ann Transl Med

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The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples

et al. 2021

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Background The stability of biological samples is vital for reliable measurements of biomarkers in large-scale survey settings, which may be affected by freeze-thaw procedures. We examined the effect of a single freeze-thaw cycle on 13 nutritional, noncommunicable diseases (NCD), and inflammatory bioanalytes in serum samples. Method Blood samples were collected from 70 subjects centrifuged after 30 minutes and aliquoted immediately. After a baseline analysis of the analytes, the samples were stored at − 70°C for 1 month and reanalyzed for all the parameters. Mean percentage differences between baseline (fresh blood) and freeze-thaw concentrations were calculated using paired sample t-tests and evaluated according to total allowable error (TEa) limits (desirable bias). Results Freeze-thaw concentrations differed significantly (p < 0.05) from baseline concentrations for soluble transferrin receptor (sTfR) (− 5.49%), vitamin D (− 12.51%), vitamin B12 (− 3.74%), plasma glucose (1.93%), C-reactive protein (CRP) (3.45%), high-density lipoprotein (HDL) (7.98%), and cholesterol (9.76%), but they were within respective TEa limits. Low-density lipoprotein (LDL) (− 0.67%), creatinine (0.94%), albumin (0.87%), total protein (1.00%), ferritin (− 0.58%), and triglycerides (TAG) (2.82%) concentrations remained stable following the freeze-thaw cycle. In conclusion, single freeze-thaw cycle of the biomarkers in serum/plasma samples after storage at − 70°C for 1 month had minimal effect on stability of the studied analytes, and the changes in concentration were within acceptable limit for all analytes.

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Influence of Freeze-Thawing and Storage on Human Serum Lipid Analytes

Cited by 2 publications

References 28 publications

Biological variation of metabolic cardiovascular risk factors in haemodialysis patients and healthy individuals

Biological variation of metabolic cardiovascular risk factors in haemodialysis patients and healthy individuals

The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples

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