Influence of Funneliformis mosseae enhanced with titanium dioxide nanoparticles (TiO2NPs) on Phaseolus vulgaris L. under salinity stress

Abstract

The Arbuscular mycorrhizal fungi (AMF) ( Funneliformis mosseae ), are the most widely distributed symbiont assisting plants to overcome counteractive environmental conditions. In order to improve the sustainability and the activity of AMF, the use of nanotechnology was important. The main objective of this study was to investigate the effect of titanium dioxide nanoparticles (TiO 2 NPs) on the activity of AMF in common bean roots as well as its activity under salinity stress using morphological and molecular methods. The activity of AMF colonization has increased in the presence of TiO 2 NPs especially for arbuscule activity (A%), which increased three times with the presence of TiO 2 NPs. The improvement rate of Funneliformis mosseae on plant growth increased from 180% to 224% of control at the lowest level of salinity and increased from 48% to 130% at higher salinity level, respectively. The AMF dependencies for plant dry biomass increased in the presence of TiO 2 NPs from 277% in the absence of salinity to 465 and 883% % at low and high salinity levels, respectively. The presence of AMF co-inoculated with TiO 2 NPs resulted in increasing the salinity tolerance of plants at all levels and reached 110% at salinity level of 100 mM NaCl. Quantitative colonization methods showed that the molecular intensity ratio and the relative density of paired inocula AMF Nest (NS) or chitin synthases gene (Chs) with TiO 2 NPs were higher significantly P.>0.05 than single inoculants of AMF gene in roots under the presence or the absence of salinity by about two folds and about 40%. Hence, the positive effect of TiO 2 NPs was confined to its effect on AMF not on bean plants itself.

Chemical Constitution and Antimicrobial Activity of Kombucha Fermented Beverage

“…The ion source temperature was set at 200 °C. Compounds were identified by comparison of their retention times and mass spectra with those of WILEY 09 and the National Institute of Standards and Technology 2011 mass spectral library (NIST 11) [ 59 , 60 ].…”

Nanotechnology in plants: recent advances and challenges

“…168 However, recent studies showed beneficial effects: Ag NPs (5 nm mean size) stimulated the formation of mycorrhizae in seedlings of pedunculate oak (Quercus robur L.), 169 and biologically synthesized TiO 2 NPs (68 nm mean size) enhanced root colonization of common bean (Phaseolus vulgaris L.) with the arbuscular mycorrhizal fungi (Funneliformis mosseae) and improved salinity tolerance compared to inoculation with arbuscular mycorrhizal fungi alone. 170 These seemingly contradictory results expose the complexity of the relations involved and highlight the necessity to consider every experimental parameterin particular, precise optimization of doses and modes of application. 169,170…”

“…170 These seemingly contradictory results expose the complexity of the relations involved and highlight the necessity to consider every experimental parameterin particular, precise optimization of doses and modes of application. 169,170…”

Assessment the using of silica nanoparticles (SiO2NPs) biosynthesized from rice husks by Trichoderma harzianum MF780864 as water lead adsorbent for immune status of Nile tilapia (Oreochromis niloticus)

“…In this framework, nanotechnology offers the ability to control matter at the nanoscale to have unique physical or chemical merits due to their small diameter, morphology or large surface area ( El-Gazzar and Enan, 2020 , El-Gazzar and Ismail, 2020 ). Nanotechnology is also used to prevent the formation of pollutants to treat and remediate contaminated water or contaminants by applying the material technology, industrial processes and others ( El-Gazzar et al, 2020 ). Biosyntheses applied by fungi might appear attractive for nanoparticles in wider rate because they have a potentiality with standing during different conditions of bioprocesses due to their filamentous nature ( Kar et al, 2014 ).…”