Influence of Genistein on the Structure of Nucleosomes and Formation of Complexes With PARP1

Abstract

The plant-derived polyphenol genistein has high biological activity, which stimulates the study of its applicability for the prevention and treatment of cancer, cardiovascular and neurodegenerative diseases. Given the ability of genistein to bind DNA, the present work examined the interaction of genistein with chromatosomes, nucleosomes, and nucleosome complexes with poly(ADP-ribose) polymerase 1 (PARP1). It has been established that over a wide range of concentrations, genistein does not affect the structure of nucleosomal DNA, but at high concentrations it causes a change in the structure of linker DNA, bringing DNA helices closer together. In chromatosomes, genistein does not cause dissociation of linker histone and changes in conformation in the region of linker DNA. At high concentrations, genistein hinders the formation of nucleosome complexes with PARP1.